Difference between revisions of "Team:SDSZ China/Demonstrate"

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<p class="title">The preprocessing of milk:</p>
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<p>Here you can describe the results of your project and your future plans. </p>
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                    <br/>
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                    <img src="https://static.igem.org/mediawiki/2016/3/31/T--SDSZ_China--preprocess.jpg" style="height: auto; width: 15vw; left:0;">
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<h5>What should this page contain?</h5>
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                    <br/><br/><br/>
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                    <p>We add milk sample into the centrifuge tube and add the equal amount of tris-saturated phenol into the milk. Then we add four times volume of chloroform into the mixture, and centrifuge the tube at 10000rpm for 5 min. (<a href="https://static.igem.org/mediawiki/2016/a/a1/T--SDSZ_China--protocol3.pdf">See Protocol</a>) The result of the preprocessing was showed in the picture above.
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<li> Clearly and objectively describe the results of your work.</li>
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                    </p><br/>
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<li> Future plans for the project </li>
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                    <p class="title">Demonstration:</p><br/>
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<li> Considerations for replicating the experiments </li>
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                    <img src="https://static.igem.org/mediawiki/2016/5/53/T--SDSZ_China--ptable5.png" style="height: auto; width: 50vw; left:0;">
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                    <img src="https://static.igem.org/mediawiki/2016/6/66/T--SDSZ_China--pfigure3.png" style="height: auto; width: 50vw; left:0;">
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                    <img src="https://static.igem.org/mediawiki/2016/0/0a/T--SDSZ_China--pfigure4.png" style="height: auto; width: 50vw; left:0;">
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                    <p class="title">Interpretation:</p>
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                    <br/><p>The last competitive binding experiment is conducted under the condition of 25 times diluted PBP5-GFP solution and preprocessed milk (containing PBP-GFP) and 405nm excitation wavelength (<a href="https://static.igem.org/mediawiki/2016/4/47/T--SDSZ_China--protocol2.pdf">See more in protocol</a>). The results indicate that the fluorescence intensity measured and penicillin concentration has a correlation factor of 0.8212211, which means the two factors is strong correlation, proving that our method doesn’t just work under the most simplified conditions and has the ability to detect penicillin in milk samples.
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<h5> Project Achievements </h5>
 
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<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
 
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<li>A list of linked bullet points of the successful results during your project</li>
 
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<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
 
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<h5>Inspiration</h5>
 
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<p>See how other teams presented their results.</p>
 
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<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
 
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<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
 
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<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
 
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Latest revision as of 21:08, 19 October 2016

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The preprocessing of milk:





We add milk sample into the centrifuge tube and add the equal amount of tris-saturated phenol into the milk. Then we add four times volume of chloroform into the mixture, and centrifuge the tube at 10000rpm for 5 min. (See Protocol) The result of the preprocessing was showed in the picture above.


Demonstration:











Interpretation:


The last competitive binding experiment is conducted under the condition of 25 times diluted PBP5-GFP solution and preprocessed milk (containing PBP-GFP) and 405nm excitation wavelength (See more in protocol). The results indicate that the fluorescence intensity measured and penicillin concentration has a correlation factor of 0.8212211, which means the two factors is strong correlation, proving that our method doesn’t just work under the most simplified conditions and has the ability to detect penicillin in milk samples.