Difference between revisions of "Team:ShanghaitechChina/Description"
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This year, we have done the two improvement work:<p></p> | This year, we have done the two improvement work:<p></p> | ||
| − | 1. We | + | 1. We improved the characterization of biobrick: <a href="http://parts.igem.org/Part:BBa_K1583003">BBa_K1583003</a> which was originally characterized by iGEM15_TU_Delft.<p></p> |
| − | 2. | + | 2. We optimized the codons for a major functional part of Hydrogenase, HydA. This biobrick:<a href="http://parts.igem.org/Part:BBa_K535002">BBa_K535002</a> was originally designed by: iGEM11_UNAM-Genomics_ Mexico. <p></p> |
| − | + | In the following content, we introduce our work in detail to illustrate why we think we met the criteria.<p></p><p></p> | |
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<div id="p1" class="content"> | <div id="p1" class="content"> | ||
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<li> Group: ShanghaitechChina</li> | <li> Group: ShanghaitechChina</li> | ||
<li> Author: Lechen Qian, Shijie Gu</li> | <li> Author: Lechen Qian, Shijie Gu</li> | ||
| − | <li> Summary: We created new way to characterize this biobrick by | + | <li> Summary: We created new way to characterize this biobrick which was originally designed and characterized by iGEM15_TU_Delft. We utilize NTA-Metal-Histag coordination chemistry and fluorescence emission traits of Quantum Dots (QDs) in our project to improve the characterization. We demonstrated the validity of the approach for measurement of biofilm composed by CsgA-His density of <i>E. coli</i> curli system and think highly of this characterization for its general application in other biofilm systems. Also, we utilized TEM to help us scrutinize the binding effect in microscopic world.</li> |
</ul></h4> | </ul></h4> | ||
<h3>>Improvement:</h3> | <h3>>Improvement:</h3> | ||
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<li> Group: ShanghaitechChina</li> | <li> Group: ShanghaitechChina</li> | ||
<li> Author: Yifan Chen</li> | <li> Author: Yifan Chen</li> | ||
| − | <li> Summary: We optimized [FeFe] Hydrogenases originally from the bacterium Clostridium acetobutylicum (Original coding sequence: hydA, <a href="http://parts.igem.org/Part:BBa_K535002">BBa_K535002</a>, designed by: iGEM11_UNAM-Genomics_ Mexico. Optimized coding sequence: hydA with SpyTag and Histag <a href="http://parts.igem.org/Part:BBa_K2132005">BBa_K2132005</a>) to accept electrons and therefor enable catalytic production of hydrogen in our project. The optimized coding sequence would produce more protein, theoretically. And optimization also improved the activity of [FeFe] Hydrogenases according to the experiment that we did.</li> | + | <li> Summary: We optimized [FeFe] Hydrogenases originally from the bacterium <i>Clostridium acetobutylicum</i> (Original coding sequence: hydA, <a href="http://parts.igem.org/Part:BBa_K535002">BBa_K535002</a>, designed by: iGEM11_UNAM-Genomics_ Mexico. Optimized coding sequence: hydA with SpyTag and Histag <a href="http://parts.igem.org/Part:BBa_K2132005">BBa_K2132005</a>) to accept electrons and therefor enable catalytic production of hydrogen in our project. The optimized coding sequence would produce more protein, theoretically. And optimization also improved the activity of [FeFe] Hydrogenases according to the experiment that we did.</li> |
</ul></h4> | </ul></h4> | ||
<h3>>Improvement:</h3> | <h3>>Improvement:</h3> | ||
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<table align="center" border="0" cellpadding="0" cellspacing="0" class="table table-hover"> | <table align="center" border="0" cellpadding="0" cellspacing="0" class="table table-hover"> | ||
| − | + | <thead> | |
| − | + | <tr> | |
| − | + | <th><strong> </strong></th> | |
| − | + | <th><strong>Max Direct Repeat</strong></th> | |
| − | + | <th><strong>Max Inverted Repeat</strong></th> | |
| − | + | <th><strong>Max Dyad Repeat</strong></th> | |
| − | + | </tr> | |
| − | + | </thead> | |
<tbody> | <tbody> | ||
| − | + | <tr> | |
| − | + | <td>After Optimization</a></td> | |
| − | + | <td>Size:15 Distance:3 Frequency:2</td> | |
| − | + | <td>None</td> | |
| − | + | <td>None</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>Before Optimization</a></td> | |
| − | + | <td>Size:16 Distance:231 Frequency:2</td> | |
| − | + | <td>None</td> | |
| − | + | <td>Size: 13 Tm: 34.6 Start Positions: 680, 1357</td> | |
| − | + | </tr> | |
<tr> | <tr> | ||
| − | + | <td colspan="12" align="center"><strong>Table 1: Removed repeat sequences information</strong></td> | |
| − | + | </tr> | |
| − | + | </tbody> | |
</table> | </table> | ||
Latest revision as of 22:56, 19 October 2016
