Difference between revisions of "Team:SUSTech Shenzhen/Parts"

 
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This year, we totally submitted 27 BioBricks, including 15 basic Parts and 12 composite Parts. We always want to contribute all of the components used in our programs and we welcome all researchers to use, measure and modify our Parts for their projects.
 +
We spared no effort do diversify our BioBricks and we successfully constructed BioBricks of several categories as listed below:
  
 +
= 1. Basic Parts:  =
  
 +
=== TRPC5 family: ===
 +
Sound sensitive channel TRPC5 gene coding sequence with or without mutagenesis on ankyrin repeats region through direct evolution.
 +
This set is also our Part Collection.
  
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<html><a href="/ Team:SUSTech_Shenzhen/Part_Collection" class="btn btn-default"><i class="ion-arrow-right-c"></i> Parts Collection</a></html>
  
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=== R-GECO coding sequence: ===
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Intracellular calcium indicator gene coding sequence with site-directed mutation on 2 PstI and 1 EcoRI recognition sites in original sequence.
 +
 +
 +
=== TetOn regulation system: ===
 +
Tetracycline-responsive promoter whose downstream gene expression can be induced by doxycycline.
 +
 +
 +
=== Antibiotics resistance gene: ===
 +
Coding sequence of blasticidin S HCl nucleoside antibiotic/ phleomycin D1/puromycin resistance genes with or without 2A sequence that can be used for eukaryotic cell selection.
 +
 +
 +
=== Mutated sfGFP coding sequence: ===
 +
Green fluorescence gene coding sequence with site-directed mutation on KpnI restriction site.
 +
 +
<html><a href="/ Team:SUSTech_Shenzhen/Basic_Part" class="btn btn-default"><i class="ion-arrow-right-c"></i> Basic Part</a></html>
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 +
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= 2. Composite Parts:  =
 +
 +
=== Chromoproteins: ===
 +
Yellow/Red/Pink/Purple chromoprotein reporter system driven by Strong Promoter-Strong RBS or Strong Promoter-Strong RBS.
 +
 +
 +
=== sfGFP and mutated sfGFP: ===
 +
Green fluorescence reporter system driven by Strong Promoter+ Strong RBS with or without site-directed mutation on KpnI restriction site in sfGFP coding sequence.
 +
 +
<html><a href="/ Team:SUSTech_Shenzhen/Composite_Part" class="btn btn-default"><i class="ion-arrow-right-c"></i> Composite Part</a></html>
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 +
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= Part List=
 +
 +
{|class="table table-striped"
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! '''Type'''
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! '''Name'''
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! '''Description'''
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! '''Length'''
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! '''Characteristics'''
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|-
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| Basic Parts
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| <partinfo>BBa_K1943012</partinfo>
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| msfGFP CDS
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| 717bp
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| Mutated sfGFP coding sequence without KpnI recognition site
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|-
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|
 +
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| <partinfo>BBa_K1943013</partinfo>
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| Puro CDS
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| 597bp
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| Puromycin resistance gene
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|-
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|
 +
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| <partinfo>BBa_K1943014</partinfo>
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| Bleo CDS
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| 369bp
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| Resistance gene to antibiotic phleomycin D1
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|-
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|
 +
 +
| <partinfo>BBa_K1943015</partinfo>
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| Bla CDS
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| 402bp
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| Coding sequence of Blasticidin S deaminase, resistant to blasticidin S HCl nucleoside antibiotic
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|-
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|
 +
 +
| <partinfo>BBa_K1943016</partinfo>
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| Bla+T2A
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| 456bp
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| Anti-antibiotics genes CDS with 2A sequence for selection when ligated with core sequence
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|-
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|
 +
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| <partinfo>BBa_K1943017</partinfo>
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| Puro+T2A
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| 651bp
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| Anti-antibiotics genes CDS with 2A sequence for selection when ligated with core sequence
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|-
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|
 +
 +
| <partinfo>BBa_K1943018</partinfo>
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| T2A+Bleo
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| 429bp
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| Anti-antibiotics genes CDS with 2A sequence for selection when ligated with core sequence
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|-
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|
 +
 +
| <partinfo>BBa_K1943019</partinfo>
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| SV40 promoter + puro anti-antibiotics gene+ 2A + TetOn 3G promoter
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| 1774bp
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| Tetracycline-responsive promoter, expression induced by doxycycline
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|-
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|
 +
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| <partinfo>BBa_K1943020</partinfo>
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| mR-GECO CDS
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| 1257bp
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| Mutated R-GECO CDS without 2 PstI in original sequence
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|-
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|
 +
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| <partinfo>BBa_K1943021</partinfo>
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| TRPC5
 +
 +
| 2928bp
 +
 +
| original TRPC5 CDS
 +
 +
|-
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|
 +
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| <partinfo>BBa_K1943022</partinfo>
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| TRPC5
 +
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| 2928bp
 +
 +
| mutated TRPC5 CDS -7
 +
 +
|-
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|
 +
 +
| <partinfo>BBa_K1943023</partinfo>
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| TRPC5
 +
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| 2928bp
 +
 +
| mutated TRPC5 CDS -12
 +
 +
|-
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|
 +
 +
| <partinfo>BBa_K1943024</partinfo>
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| TRPC5
 +
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| 2928bp
 +
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| mutated TRPC5 CDS -15
 +
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|-
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|
 +
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| <partinfo>BBa_K1943025</partinfo>
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| TRPC5
 +
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| 2928bp
 +
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| mutated TRPC5 CDS -18
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|-
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|
 +
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| <partinfo>BBa_K1943026</partinfo>
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| TRPC5
 +
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| 2928bp
 +
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| mutated TRPC5 CDS -20
 +
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|-
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| Composite Parts
 +
| <partinfo>BBa_K1943000</partinfo>
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| J23100+ B0034+ fwYellow+ B0010+ B0012
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| 912bp
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| Yellow chromoprotein reporter system (Strong Promoter, Strong RBS)
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|-
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|
 +
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| <partinfo>BBa_K1943001</partinfo>
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| J23110+ B0031+ fwYellow+ B0010+ B0012
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| 914bp
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| Yellow chromoprotein reporter system (medium Promoter , weak RBS)
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|-
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|
 +
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| <partinfo>BBa_K1943002</partinfo>
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| J23100+ B0034+ gfasPurple + B0010+ B0012
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| 867bp
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| Purple chromoprotein reporter system (Strong Promoter, Strong RBS)
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|-
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|
 +
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| <partinfo>BBa_K1943003</partinfo>
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| J23110+ B0031+ gfasPurplee + B0010+ B0012
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| 869bp
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| Purple chromoprotein reporter system (medium Promoter , weak RBS)
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|-
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|
 +
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| <partinfo>BBa_K1943004</partinfo>
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| J23100+ B0034+ eforRed + B0010+ B0012
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| 879bp
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| Red chromoprotein reporter system (Strong Promoter, Strong RBS)
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|-
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|
 +
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| <partinfo>BBa_K1943005</partinfo>
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| J23110+ B0031+ eforRed + B0010+ B0012
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| 881bp
 +
| Red chromoprotein reporter system (medium Promoter , weak RBS)
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|-
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|
 +
 +
| <partinfo>BBa_K1943006</partinfo>
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| J23100+ B0034+ spisPink + B0010+ B0012
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| 876bp
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| Pink chromoprotein reporter system (Strong Promoter, Strong RBS)
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|-
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|
 +
 +
| <partinfo>BBa_K1943007</partinfo>
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| J23110+ B0031+ spisPink + B0010+ B0012
 +
| 878bp
 +
| Pink chromoprotein reporter system (medium Promoter , weak RBS)
 +
|-
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|
 +
 +
| <partinfo>BBa_K1943008</partinfo>
 +
| J23116+B0034+sfGFP
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| 778bp
 +
| Strong green fluorescence
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|-
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|
 +
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| <partinfo>BBa_K1943009</partinfo>
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| J23116+B0034+sfGFP+ B0010+B0012
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| 918bp
 +
| Strong green fluorescence
 +
|-
 +
|
 +
 +
| <partinfo>BBa_K1943010</partinfo>
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| J23116+B0034+msfGFP
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| 781bp
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| Strong green fluorescence without KpnI recognition site in CDS
 +
|-
 +
|
 +
 +
| <partinfo>BBa_K1943011</partinfo>
 +
| J23116+B0034+msfGFP+ B0010+B0012
 +
| 915bp
 +
| Strong green fluorescence without KpnI recognition site in CDS
 +
|}
 
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Latest revision as of 01:15, 20 October 2016

Team SUSTC-Shenzhen

Parts

Contribution

This year, we totally submitted 27 BioBricks, including 15 basic Parts and 12 composite Parts. We always want to contribute all of the components used in our programs and we welcome all researchers to use, measure and modify our Parts for their projects. We spared no effort do diversify our BioBricks and we successfully constructed BioBricks of several categories as listed below:

1. Basic Parts:

TRPC5 family:

Sound sensitive channel TRPC5 gene coding sequence with or without mutagenesis on ankyrin repeats region through direct evolution. This set is also our Part Collection.

Parts Collection

R-GECO coding sequence:

Intracellular calcium indicator gene coding sequence with site-directed mutation on 2 PstI and 1 EcoRI recognition sites in original sequence.


TetOn regulation system:

Tetracycline-responsive promoter whose downstream gene expression can be induced by doxycycline.


Antibiotics resistance gene:

Coding sequence of blasticidin S HCl nucleoside antibiotic/ phleomycin D1/puromycin resistance genes with or without 2A sequence that can be used for eukaryotic cell selection.


Mutated sfGFP coding sequence:

Green fluorescence gene coding sequence with site-directed mutation on KpnI restriction site.

Basic Part


2. Composite Parts:

Chromoproteins:

Yellow/Red/Pink/Purple chromoprotein reporter system driven by Strong Promoter-Strong RBS or Strong Promoter-Strong RBS.


sfGFP and mutated sfGFP:

Green fluorescence reporter system driven by Strong Promoter+ Strong RBS with or without site-directed mutation on KpnI restriction site in sfGFP coding sequence.

Composite Part


Part List

Type Name Description Length Characteristics
Basic Parts <partinfo>BBa_K1943012</partinfo> msfGFP CDS 717bp Mutated sfGFP coding sequence without KpnI recognition site
<partinfo>BBa_K1943013</partinfo> Puro CDS 597bp Puromycin resistance gene
<partinfo>BBa_K1943014</partinfo> Bleo CDS 369bp Resistance gene to antibiotic phleomycin D1
<partinfo>BBa_K1943015</partinfo> Bla CDS 402bp Coding sequence of Blasticidin S deaminase, resistant to blasticidin S HCl nucleoside antibiotic
<partinfo>BBa_K1943016</partinfo> Bla+T2A 456bp Anti-antibiotics genes CDS with 2A sequence for selection when ligated with core sequence
<partinfo>BBa_K1943017</partinfo> Puro+T2A 651bp Anti-antibiotics genes CDS with 2A sequence for selection when ligated with core sequence
<partinfo>BBa_K1943018</partinfo> T2A+Bleo 429bp Anti-antibiotics genes CDS with 2A sequence for selection when ligated with core sequence
<partinfo>BBa_K1943019</partinfo> SV40 promoter + puro anti-antibiotics gene+ 2A + TetOn 3G promoter 1774bp Tetracycline-responsive promoter, expression induced by doxycycline
<partinfo>BBa_K1943020</partinfo> mR-GECO CDS 1257bp Mutated R-GECO CDS without 2 PstI in original sequence
<partinfo>BBa_K1943021</partinfo> TRPC5 2928bp original TRPC5 CDS
<partinfo>BBa_K1943022</partinfo> TRPC5 2928bp mutated TRPC5 CDS -7
<partinfo>BBa_K1943023</partinfo> TRPC5 2928bp mutated TRPC5 CDS -12
<partinfo>BBa_K1943024</partinfo> TRPC5 2928bp mutated TRPC5 CDS -15
<partinfo>BBa_K1943025</partinfo> TRPC5 2928bp mutated TRPC5 CDS -18
<partinfo>BBa_K1943026</partinfo> TRPC5 2928bp mutated TRPC5 CDS -20
Composite Parts <partinfo>BBa_K1943000</partinfo> J23100+ B0034+ fwYellow+ B0010+ B0012 912bp Yellow chromoprotein reporter system (Strong Promoter, Strong RBS)
<partinfo>BBa_K1943001</partinfo> J23110+ B0031+ fwYellow+ B0010+ B0012 914bp Yellow chromoprotein reporter system (medium Promoter , weak RBS)
<partinfo>BBa_K1943002</partinfo> J23100+ B0034+ gfasPurple + B0010+ B0012 867bp Purple chromoprotein reporter system (Strong Promoter, Strong RBS)
<partinfo>BBa_K1943003</partinfo> J23110+ B0031+ gfasPurplee + B0010+ B0012 869bp Purple chromoprotein reporter system (medium Promoter , weak RBS)
<partinfo>BBa_K1943004</partinfo> J23100+ B0034+ eforRed + B0010+ B0012 879bp Red chromoprotein reporter system (Strong Promoter, Strong RBS)
<partinfo>BBa_K1943005</partinfo> J23110+ B0031+ eforRed + B0010+ B0012 881bp Red chromoprotein reporter system (medium Promoter , weak RBS)
<partinfo>BBa_K1943006</partinfo> J23100+ B0034+ spisPink + B0010+ B0012 876bp Pink chromoprotein reporter system (Strong Promoter, Strong RBS)
<partinfo>BBa_K1943007</partinfo> J23110+ B0031+ spisPink + B0010+ B0012 878bp Pink chromoprotein reporter system (medium Promoter , weak RBS)
<partinfo>BBa_K1943008</partinfo> J23116+B0034+sfGFP 778bp Strong green fluorescence
<partinfo>BBa_K1943009</partinfo> J23116+B0034+sfGFP+ B0010+B0012 918bp Strong green fluorescence
<partinfo>BBa_K1943010</partinfo> J23116+B0034+msfGFP 781bp Strong green fluorescence without KpnI recognition site in CDS
<partinfo>BBa_K1943011</partinfo> J23116+B0034+msfGFP+ B0010+B0012 915bp Strong green fluorescence without KpnI recognition site in CDS

Made by from the iGEM team SUSTech_Shenzhen.

Licensed under CC BY 4.0.

  • ©2016 SUSTech_Shenzhen