Difference between revisions of "Team:SUSTech Shenzhen/Parts"

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This year, we totally submitted 27 BioBricks, including 15 basic Parts and 12 composite Parts. We always want to contribute all of the components used in our programs and we welcome all researchers to use, measure and modify our Parts for their projects.
 +
We spared no effort do diversify our BioBricks and we successfully constructed BioBricks of several categories as listed below:
  
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= 1. Basic Parts:  =
  
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=== TRPC5 family: ===
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Sound sensitive channel TRPC5 gene coding sequence with or without mutagenesis on ankyrin repeats region through direct evolution.
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This set is also our Part Collection.
  
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<html><a href="/ Team:SUSTech_Shenzhen/Part_Collection" class="btn btn-default"><i class="ion-arrow-right-c"></i> Parts Collection</a></html>
  
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=== R-GECO coding sequence: ===
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Intracellular calcium indicator gene coding sequence with site-directed mutation on 2 PstI and 1 EcoRI recognition sites in original sequence.
  
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<div class="column full_size">
 
  
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=== TetOn regulation system: ===
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Tetracycline-responsive promoter whose downstream gene expression can be induced by doxycycline.
  
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
 
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
 
  
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=== Antibiotics resistance gene: ===
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Coding sequence of blasticidin S HCl nucleoside antibiotic/ phleomycin D1/puromycin resistance genes with or without 2A sequence that can be used for eukaryotic cell selection.
  
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</div>
 
  
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=== Mutated sfGFP coding sequence: ===
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Green fluorescence gene coding sequence with site-directed mutation on KpnI restriction site.
  
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<html><a href="/ Team:SUSTech_Shenzhen/Basic_Part" class="btn btn-default"><i class="ion-arrow-right-c"></i> Basic Part</a></html>
  
  
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= 2. Composite Parts:  =
  
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<div class="column half_size">
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=== Chromoproteins: ===
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<div class="highlight">
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Yellow/Red/Pink/Purple chromoprotein reporter system driven by Strong Promoter-Strong RBS or Strong Promoter-Strong RBS.
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<h5>Note</h5>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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</div>
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</div>
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=== sfGFP and mutated sfGFP: ===
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Green fluorescence reporter system driven by Strong Promoter+ Strong RBS with or without site-directed mutation on KpnI restriction site in sfGFP coding sequence.
  
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<html><a href="/ Team:SUSTech_Shenzhen/Composite_Part" class="btn btn-default"><i class="ion-arrow-right-c"></i> Composite Part</a></html>
  
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<div class="column half_size">
 
  
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<h5>Adding parts to the registry</h5>
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= Part List=
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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</div>
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{|class="table table-striped"
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! '''Type'''
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! '''Name'''
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! '''Description'''
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! '''Length'''
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! '''Characteristics'''
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|-
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| Basic Parts
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| <partinfo>BBa_K1943012</partinfo>
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| msfGFP CDS
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| 717bp
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| Mutated sfGFP coding sequence without KpnI recognition site
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|-
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|
  
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| <partinfo>BBa_K1943013</partinfo>
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| Puro CDS
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| 597bp
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| Puromycin resistance gene
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|-
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|
  
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| <partinfo>BBa_K1943014</partinfo>
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| Bleo CDS
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| 369bp
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| Resistance gene to antibiotic phleomycin D1
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|-
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|
  
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| <partinfo>BBa_K1943015</partinfo>
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| Bla CDS
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| 402bp
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| Coding sequence of Blasticidin S deaminase, resistant to blasticidin S HCl nucleoside antibiotic
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|-
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|
  
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<div class="column half_size">
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| <partinfo>BBa_K1943016</partinfo>
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| Bla+T2A
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| 456bp
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| Anti-antibiotics genes CDS with 2A sequence for selection when ligated with core sequence
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|-
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|
  
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<h5>What information do I need to start putting my parts on the Registry?</h5>
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| <partinfo>BBa_K1943017</partinfo>
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<p>The information needed to initially create a part on the Registry is:</p>
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| Puro+T2A
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<ul>
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| 651bp
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<li>Part Name</li>
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| Anti-antibiotics genes CDS with 2A sequence for selection when ligated with core sequence
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<li>Part type</li>
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|-
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<li>Creator</li>
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|
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<li>Sequence</li>
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<li>Short Description (60 characters on what the DNA does)</li>
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<li>Long Description (Longer description of what the DNA does)</li>
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<li>Design considerations</li>
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</ul>
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<p>
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| <partinfo>BBa_K1943018</partinfo>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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| T2A+Bleo
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| 429bp
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| Anti-antibiotics genes CDS with 2A sequence for selection when ligated with core sequence
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|-
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|
  
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</div>
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| <partinfo>BBa_K1943019</partinfo>
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| SV40 promoter + puro anti-antibiotics gene+ 2A + TetOn 3G promoter
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| 1774bp
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| Tetracycline-responsive promoter, expression induced by doxycycline
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|-
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|
  
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| <partinfo>BBa_K1943020</partinfo>
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| mR-GECO CDS
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| 1257bp
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| Mutated R-GECO CDS without 2 PstI in original sequence
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|-
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|
  
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<div class="column half_size">
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| <partinfo>BBa_K1943021</partinfo>
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| TRPC5
  
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<h5>Inspiration</h5>
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| 2928bp
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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| original TRPC5 CDS
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<ul>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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</ul>
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</div>
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<div class="column full_size">
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|-
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<h5>Part Table </h5>
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|
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<div class="highlight">
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 +
| <partinfo>BBa_K1943022</partinfo>
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| TRPC5
  
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</html>
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| 2928bp
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<groupparts>iGEM2016 Example</groupparts>
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<html>
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</div>
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</div>
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 +
| mutated TRPC5 CDS -7
  
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|-
 +
|
  
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| <partinfo>BBa_K1943023</partinfo>
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| TRPC5
  
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</html>
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| 2928bp
 +
 
 +
| mutated TRPC5 CDS -12
 +
 
 +
|-
 +
|
 +
 
 +
| <partinfo>BBa_K1943024</partinfo>
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| TRPC5
 +
 
 +
| 2928bp
 +
 
 +
| mutated TRPC5 CDS -15
 +
 
 +
|-
 +
|
 +
 
 +
| <partinfo>BBa_K1943025</partinfo>
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| TRPC5
 +
 
 +
| 2928bp
 +
 
 +
| mutated TRPC5 CDS -18
 +
 
 +
|-
 +
|
 +
 
 +
| <partinfo>BBa_K1943026</partinfo>
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| TRPC5
 +
 
 +
| 2928bp
 +
 
 +
| mutated TRPC5 CDS -20
 +
 
 +
|-
 +
| Composite Parts
 +
| <partinfo>BBa_K1943000</partinfo>
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| J23100+ B0034+ fwYellow+ B0010+ B0012
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| 912bp
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| Yellow chromoprotein reporter system (Strong Promoter, Strong RBS)
 +
|-
 +
|
 +
 
 +
| <partinfo>BBa_K1943001</partinfo>
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| J23110+ B0031+ fwYellow+ B0010+ B0012
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| 914bp
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| Yellow chromoprotein reporter system (medium Promoter , weak RBS)
 +
|-
 +
|
 +
 
 +
| <partinfo>BBa_K1943002</partinfo>
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| J23100+ B0034+ gfasPurple + B0010+ B0012
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| 867bp
 +
| Purple chromoprotein reporter system (Strong Promoter, Strong RBS)
 +
|-
 +
|
 +
 
 +
| <partinfo>BBa_K1943003</partinfo>
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| J23110+ B0031+ gfasPurplee + B0010+ B0012
 +
| 869bp
 +
| Purple chromoprotein reporter system (medium Promoter , weak RBS)
 +
|-
 +
|
 +
 
 +
| <partinfo>BBa_K1943004</partinfo>
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| J23100+ B0034+ eforRed + B0010+ B0012
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| 879bp
 +
| Red chromoprotein reporter system (Strong Promoter, Strong RBS)
 +
|-
 +
|
 +
 
 +
| <partinfo>BBa_K1943005</partinfo>
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| J23110+ B0031+ eforRed + B0010+ B0012
 +
| 881bp
 +
| Red chromoprotein reporter system (medium Promoter , weak RBS)
 +
|-
 +
|
 +
 
 +
| <partinfo>BBa_K1943006</partinfo>
 +
| J23100+ B0034+ spisPink + B0010+ B0012
 +
| 876bp
 +
| Pink chromoprotein reporter system (Strong Promoter, Strong RBS)
 +
|-
 +
|
 +
 
 +
| <partinfo>BBa_K1943007</partinfo>
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| J23110+ B0031+ spisPink + B0010+ B0012
 +
| 878bp
 +
| Pink chromoprotein reporter system (medium Promoter , weak RBS)
 +
|-
 +
|
 +
 
 +
| <partinfo>BBa_K1943008</partinfo>
 +
| J23116+B0034+sfGFP
 +
| 778bp
 +
| Strong green fluorescence
 +
|-
 +
|
 +
 
 +
| <partinfo>BBa_K1943009</partinfo>
 +
| J23116+B0034+sfGFP+ B0010+B0012
 +
| 918bp
 +
| Strong green fluorescence
 +
|-
 +
|
 +
 
 +
| <partinfo>BBa_K1943010</partinfo>
 +
| J23116+B0034+msfGFP
 +
| 781bp
 +
| Strong green fluorescence without KpnI recognition site in CDS
 +
|-
 +
|
 +
 
 +
| <partinfo>BBa_K1943011</partinfo>
 +
| J23116+B0034+msfGFP+ B0010+B0012
 +
| 915bp
 +
| Strong green fluorescence without KpnI recognition site in CDS
 +
|}
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{{:Team:SUSTech_Shenzhen/main-content-end}}
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{{:Team:SUSTech_Shenzhen/wiki-footer}}
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{{:Team:SUSTech_Shenzhen/themeJs}}

Latest revision as of 01:15, 20 October 2016

Team SUSTC-Shenzhen

Parts

Contribution

This year, we totally submitted 27 BioBricks, including 15 basic Parts and 12 composite Parts. We always want to contribute all of the components used in our programs and we welcome all researchers to use, measure and modify our Parts for their projects. We spared no effort do diversify our BioBricks and we successfully constructed BioBricks of several categories as listed below:

1. Basic Parts:

TRPC5 family:

Sound sensitive channel TRPC5 gene coding sequence with or without mutagenesis on ankyrin repeats region through direct evolution. This set is also our Part Collection.

Parts Collection

R-GECO coding sequence:

Intracellular calcium indicator gene coding sequence with site-directed mutation on 2 PstI and 1 EcoRI recognition sites in original sequence.


TetOn regulation system:

Tetracycline-responsive promoter whose downstream gene expression can be induced by doxycycline.


Antibiotics resistance gene:

Coding sequence of blasticidin S HCl nucleoside antibiotic/ phleomycin D1/puromycin resistance genes with or without 2A sequence that can be used for eukaryotic cell selection.


Mutated sfGFP coding sequence:

Green fluorescence gene coding sequence with site-directed mutation on KpnI restriction site.

Basic Part


2. Composite Parts:

Chromoproteins:

Yellow/Red/Pink/Purple chromoprotein reporter system driven by Strong Promoter-Strong RBS or Strong Promoter-Strong RBS.


sfGFP and mutated sfGFP:

Green fluorescence reporter system driven by Strong Promoter+ Strong RBS with or without site-directed mutation on KpnI restriction site in sfGFP coding sequence.

Composite Part


Part List

Type Name Description Length Characteristics
Basic Parts <partinfo>BBa_K1943012</partinfo> msfGFP CDS 717bp Mutated sfGFP coding sequence without KpnI recognition site
<partinfo>BBa_K1943013</partinfo> Puro CDS 597bp Puromycin resistance gene
<partinfo>BBa_K1943014</partinfo> Bleo CDS 369bp Resistance gene to antibiotic phleomycin D1
<partinfo>BBa_K1943015</partinfo> Bla CDS 402bp Coding sequence of Blasticidin S deaminase, resistant to blasticidin S HCl nucleoside antibiotic
<partinfo>BBa_K1943016</partinfo> Bla+T2A 456bp Anti-antibiotics genes CDS with 2A sequence for selection when ligated with core sequence
<partinfo>BBa_K1943017</partinfo> Puro+T2A 651bp Anti-antibiotics genes CDS with 2A sequence for selection when ligated with core sequence
<partinfo>BBa_K1943018</partinfo> T2A+Bleo 429bp Anti-antibiotics genes CDS with 2A sequence for selection when ligated with core sequence
<partinfo>BBa_K1943019</partinfo> SV40 promoter + puro anti-antibiotics gene+ 2A + TetOn 3G promoter 1774bp Tetracycline-responsive promoter, expression induced by doxycycline
<partinfo>BBa_K1943020</partinfo> mR-GECO CDS 1257bp Mutated R-GECO CDS without 2 PstI in original sequence
<partinfo>BBa_K1943021</partinfo> TRPC5 2928bp original TRPC5 CDS
<partinfo>BBa_K1943022</partinfo> TRPC5 2928bp mutated TRPC5 CDS -7
<partinfo>BBa_K1943023</partinfo> TRPC5 2928bp mutated TRPC5 CDS -12
<partinfo>BBa_K1943024</partinfo> TRPC5 2928bp mutated TRPC5 CDS -15
<partinfo>BBa_K1943025</partinfo> TRPC5 2928bp mutated TRPC5 CDS -18
<partinfo>BBa_K1943026</partinfo> TRPC5 2928bp mutated TRPC5 CDS -20
Composite Parts <partinfo>BBa_K1943000</partinfo> J23100+ B0034+ fwYellow+ B0010+ B0012 912bp Yellow chromoprotein reporter system (Strong Promoter, Strong RBS)
<partinfo>BBa_K1943001</partinfo> J23110+ B0031+ fwYellow+ B0010+ B0012 914bp Yellow chromoprotein reporter system (medium Promoter , weak RBS)
<partinfo>BBa_K1943002</partinfo> J23100+ B0034+ gfasPurple + B0010+ B0012 867bp Purple chromoprotein reporter system (Strong Promoter, Strong RBS)
<partinfo>BBa_K1943003</partinfo> J23110+ B0031+ gfasPurplee + B0010+ B0012 869bp Purple chromoprotein reporter system (medium Promoter , weak RBS)
<partinfo>BBa_K1943004</partinfo> J23100+ B0034+ eforRed + B0010+ B0012 879bp Red chromoprotein reporter system (Strong Promoter, Strong RBS)
<partinfo>BBa_K1943005</partinfo> J23110+ B0031+ eforRed + B0010+ B0012 881bp Red chromoprotein reporter system (medium Promoter , weak RBS)
<partinfo>BBa_K1943006</partinfo> J23100+ B0034+ spisPink + B0010+ B0012 876bp Pink chromoprotein reporter system (Strong Promoter, Strong RBS)
<partinfo>BBa_K1943007</partinfo> J23110+ B0031+ spisPink + B0010+ B0012 878bp Pink chromoprotein reporter system (medium Promoter , weak RBS)
<partinfo>BBa_K1943008</partinfo> J23116+B0034+sfGFP 778bp Strong green fluorescence
<partinfo>BBa_K1943009</partinfo> J23116+B0034+sfGFP+ B0010+B0012 918bp Strong green fluorescence
<partinfo>BBa_K1943010</partinfo> J23116+B0034+msfGFP 781bp Strong green fluorescence without KpnI recognition site in CDS
<partinfo>BBa_K1943011</partinfo> J23116+B0034+msfGFP+ B0010+B0012 915bp Strong green fluorescence without KpnI recognition site in CDS

Made by from the iGEM team SUSTech_Shenzhen.

Licensed under CC BY 4.0.

  • ©2016 SUSTech_Shenzhen