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+ | <div class="topic"><p class="text_color">Survey Collaboration</p></div> | ||
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<p class="title">Characterization of Biobricks</p> | <p class="title">Characterization of Biobricks</p> | ||
<p class="content-1">NCTU_Formosa & Mingdao Senior High School Collaboration</p> | <p class="content-1">NCTU_Formosa & Mingdao Senior High School Collaboration</p> | ||
− | <p class="content">We | + | <p class="content">We helped Mingdao iGEM team to confirm their BioBrick. We cultivated the <i>E.coli</i> according to their protocol. This year Mingdao iGEM team constructs a biobrick that can express enzyme, alcohol oxidase (AOX), on the surface of <i>E.coli</i>. The long anchor of the <i>E.coli</i> is from the concept of 2015 NCTU_FORMOSA.</p> |
− | <p class="content">Method: We | + | <p class="content">Method: We cultivated <i>E.coli</i> BL21 3mL containing the biobrick selecting with Chloromycetin, and the other one is the control which doesn’t have the plasmid. Then we centrifuged 1mL bacteria solution and added PBS to adjust the OD values are almost the same. Then we added TMB, a substrate of oxidase and react in the dark. The product can be analysis by OD650. Ultimately, we proved their biobrick is valid.</p> |
− | <p class="content">On the other hand, Mingdao iGEM team also | + | <p class="content">On the other hand, Mingdao iGEM team also characterized our Biobrick is valid to express the target protein. They run an SDS-PAGE of our Pantide and no plasmid bacteria. The result shows that our Pantide is successfully expressed.</p> |
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− | <p class="title"> | + | <p class="title">Survey Collaboration</p> |
<p class="content">International Survey aims to investigate the background knowledge of synthetic biology and the experience about genetic engineering across the world. There are 11 iGEM teams from different continents in the collaboration. The idea of the survey was first proposed by iGEM Aachen 2016, and fortunately, we had had online meetups in August. So we gladly accepted the offer as a principal team in Asia. We surveyed people on the street. Finally, we collected more than 500 samples and got some great result. Here are the teams that participate in the survey.</p> | <p class="content">International Survey aims to investigate the background knowledge of synthetic biology and the experience about genetic engineering across the world. There are 11 iGEM teams from different continents in the collaboration. The idea of the survey was first proposed by iGEM Aachen 2016, and fortunately, we had had online meetups in August. So we gladly accepted the offer as a principal team in Asia. We surveyed people on the street. Finally, we collected more than 500 samples and got some great result. Here are the teams that participate in the survey.</p> | ||
Latest revision as of 01:33, 20 October 2016