Difference between revisions of "Team:Toulouse France/Collaborations"

 
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<p class="sec_title_trans">Collaborations</p>
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<center><p class="sec_title_trans">Collaborations and Meet Up</p></center>
 
 
 
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<br><br><b><u>Protocol videos</u></b>
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<h2>Our Collaborations</h2>
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<br>Our team decided to create protocol videos in order to help young scientists in manipulation. During our experiments, we filmed 4 steps of cloning: PCR, digestion, ligation and transformation. We asked other teams to translate protocols in their mother tongue. We obtained protocol videos in English with the Imperial College London team, in Spanish thanks to the TEC-Monterrey team, in Dutch with Leiden team, in Chinese with BIT-China team, in Portuguese thanks to EEL-USP team and in French with the collaboration of the Bordeaux team.  
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<br>We wrote the entire script and we added some guidance in red (minutes for each paragraphs for instance). Teams had to translate and record the script. Now, we possess a lot of protocols explained in six different languages, accessible to everybody on Youtube and on our wiki.
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<u><p class="title1" id="select1" style="text-align:center">Protocol videos</p></u>
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Our team decided to create protocol videos in order to help young scientists to manipulate.  
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When people are working on <b>Genetics engineering</b>, there are several <b>basic experiments</b> that have to be controlled.
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During our experiments, we filmed 4 steps of cloning: <b>PCR</b>, <b>digestion</b>, <b>ligation</b> and <b>transformation</b>.  
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<br>Watching it, people could see how to manipulate safely.
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We asked other igem teams to translate protocols in their mother tongue.  
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We obtained protocol videos in <b>English</b> with <b><a href="https://2016.igem.org/Team:Imperial_College">Imperial College</a></b> team,  
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in <b>Spanish</b> thanks to the <b><a href="https://2016.igem.org/Team:Tec-Monterrey">TEC-Monterrey</a></b> team,  
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in <b>Dutch</b> with <b><a href="https://2016.igem.org/Team:Leiden">Leiden</a></b> team,  
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in <b>Chinese</b> with <b><a href="https://2016.igem.org/Team:BIT">BIT</a></b> team,  
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in <b>Portuguese</b> thanks to <b><a href="https://2016.igem.org/Team:USP-EEL-Brazil">EEL</a></b> team,
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in <b>German</b> with <b><a href="https://2016.igem.org/Team:Hamburg">Hamburg</a></b> team and  
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in <b>French</b> with the collaboration of the <b><a href="https://2016.igem.org/Team:Bordeaux">Bordeaux</a></b> team.  
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We wrote the entire script and we added some <b>guidance</b> in red (minutes for each paragraphs for instance). Teams had to translate and record the script.
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Now, we possess a lot of protocols explained in <b>six</b> different languages, accessible to everybody on <b>Youtube</b> and on our wiki.  
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<br><br><b><u>Growth study of <i>B. subtilis</i> and <i>P. fluorescens</i></u></b>
 
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<br>In exchange for recording the script in English, we studied the optimal growth of Bacillus subtilis and Pseudomonas fluorescens for the Imperial London College team. First, we did an experimental plan to observe if pH is in interaction with temperature. Finally, the conclusion was that pH and temperature were two independent parameters. We ran the experiments again, changing only one parameter.
 
  
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<br><br><b>Results</b>
 
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<td style=" background-color:#f8f8f8;"><p class ="texte" style="text-align:center; margin: 0 10px;">Optimum Temperature (°C)</td>
 
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<td style=" background-color:#f8f8f8;"><p class ="texte" style="text-align:center; margin: 0 10px;">Optimum pH</td>
 
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<td style=" background-color:#f8f8f8;"><p class ="texte" style="text-align:center; padding-top: 42px; font-size: 17px; ">Doubling time (minutes)</td>
 
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<td style=" background-color:#f8f8f8"><p class ="texte" style="padding-top: 10px; text-align:center;"><i>Bacillus subtilis</i> (WT 168)</p></td>
 
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<td><p class ="texte" style="text-align:center">34-37</p></td>
 
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<td><p class ="texte" style="text-align:center">8</p>
 
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<td><p class ="texte" style="text-align:center">41</p></td>
 
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<td style=" background-color:#f8f8f8"><p class ="texte" style="padding-top: 10px; text-align:center;"><i>Pseudomonas fluorescens</i> (SBW25)</p></td>
 
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<td><p class ="texte" style="text-align:center"><29</p></td>
 
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<td><p class ="texte" style="text-align:center">7,5</p>
 
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<td><p class ="texte" style="text-align:center"><80</p>
 
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<br><br>Optimum temperatures and pH and the doubling times are summarized in the table for our two strains.
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<br><br><b><u>3D conception</u></b>
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<br>Bordeaux
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<h2>Digestion</h2>
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<p>This video presents the protocol to do enzymatic digestion of a DNA sample. This technique is useful for usual clonings. </p>
  
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<br><br><b><u>Receipt of plasmids</u></b>
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<br>We want to thank the 2012 Munich team for sending us 4 different shuttle plasmids. One of these, pBs0k-p, was very useful because we used it for cloning in E.coli and Bacillus subtilis.  
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<br><br><b><u>Mailing of kit</u></b>
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<br>2016 Evry team asked us to send a Ludox solution that was in our iGEM Kit. They stored their own solution at -20°C so it was not usable anymore. But it was important for them to participate to the InterLab project so we helped them.
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<a href="https://2016.igem.org/Team:Toulouse_France/Collaborations#Chine1">
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<br><br><b><u>Protocol translation</u></b>
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<br>2016 METU HS Ankara team wanted to create a database, which consists of different language protocols. They asked us to translate their protocol in French.
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<a href="https://2016.igem.org/Team:Toulouse_France/Collaborations#German1">
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<br><br><b><u>Surveys</u></b>
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<br>We filled in different surveys all along the year. Among them, surveys for:
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<ul>Bdx</ul>
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<ul>IONIS</ul>
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<ul>Evry</ul>
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<ul>Valencia</ul>
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<ul>TecCEM Collegiate</ul>
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<ul>Purdue</ul>
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<ul>UBGM</ul>
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<center><h3><a href="https://2016.igem.org/Team:Imperial_College">Imperial College of London team</a></h3></center>
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<h2>Tutorials</h2>
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<center><img src="https://static.igem.org/mediawiki/2016/7/7d/Toulouse_France_logoimperial.jpg" style="width:30%"></center>
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<h2>Digestion</h2>
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<center><h3><a href="https://2016.igem.org/Team:Leiden">Leiden Team</h3></a></center>
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<center><h3><a href="https://2016.igem.org/Team:USP-EEL-Brazil">EEL Brazil Team</h3></a></center>
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<center><img src="https://static.igem.org/mediawiki/2016/a/a5/Toulouse_France_logoeel.jpg" style="width:30%"></center>
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<center><h3><a href="https://2016.igem.org/Team:Tec-Monterrey">Tec-Monterrey Team</h3></a></center>
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<center><h3><a href="https://2016.igem.org/Team:Bordeaux">Bordeaux Team</h3></a></center>
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<center><img src="https://static.igem.org/mediawiki/2016/d/d0/Toulouse_France_logobdx.jpg" style="width:30%"></center>
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<center><h3><a href="https://2016.igem.org/Team:BIT">BIT Team</h3></a></center>
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<center><h3><a href="https://2016.igem.org/Team:Hamburg">Hamburg Team</h3></a></center>
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<h2>Ligation</h2>
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<div id=video style="margin-left:80px">  
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<p>When a DNA fragment is digested with digestion enzymes, it is possible to ligate its extremities with another digested fragment, if the extremities are cohesives. This method can be used to add an insert in a vector or a backbone. </p>
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<h3>Imperial College of London team</h3>
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<p> Tuto digestion
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</div>
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<div style="clear: both"></div>
 
</div>
 
</div>
 
 
 +
<div class="column-right">
  
−
<div class="descriptionArea2" id="Portugal1">
+
<div class="descriptionArea2" id="empty"></div>
−
+
 
−
<div id=video style="margin-left:80px">  
+
 
−
<video width="800" height="450" controls>
+
<div class="descriptionArea2" id="ImperialCollege2">
−
  <source src="https://static.igem.org/mediawiki/2016/7/75/Toulouse_France_TutoDigestion_portu.mp4" type="video/mp4">
+
<br><br>
−
Your browser does not support the video tag.
+
<div id=video style="margin-left:80px">  
−
</video>
+
<video width="490" height="275" controls>
 +
  <source src="https://static.igem.org/mediawiki/2016/4/47/Toulouse_France_TutoLigation_english.mp4" type="video/mp4">
 +
Your browser does not support the video tag.
 +
</video>
 +
<center><h3><a href="https://2016.igem.org/Team:Imperial_College">Imperial College of London team</a></h3></center>
 +
 
 +
<center><img src="https://static.igem.org/mediawiki/2016/7/7d/Toulouse_France_logoimperial.jpg" style="width:30%"></center>
 +
</div>
 
</div>
 
</div>
  
−
<h3>Imperial College of London team</h3>
+
<div class="descriptionArea2" id="Leiden2">
 +
<br><br>
 +
<div id=video style="margin-left:80px">
 +
<video width="490" height="275" controls>
 +
  <source src="https://static.igem.org/mediawiki/2016/d/d6/Toulouse_France_TutoLigation_dutch.mp4" type="video/mp4">
 +
Your browser does not support the video tag.
 +
</video>
 +
<center><h3><a href="https://2016.igem.org/Team:Leiden">Leiden Team</h3></a></center>
 +
 +
<center><img src="https://static.igem.org/mediawiki/2016/3/3f/Toulouse_France_logoleiden.jpg" style="width:30%"></center>
 +
</div>
 +
</div>
 +
 
 +
<div class="descriptionArea2" id="Brazil2">
 +
<br><br>
 +
<div id=video style="margin-left:80px">
 +
<video width="490" height="275" controls>
 +
  <source src="https://static.igem.org/mediawiki/2016/7/71/Toulouse_France_TutoLigation_portu.mp4" type="video/mp4">
 +
Your browser does not support the video tag.
 +
</video>
 +
<center><h3><a href="https://2016.igem.org/Team:USP-EEL-Brazil">EEL Brazil Team</h3></a></center>
 +
 +
<center><img src="https://static.igem.org/mediawiki/2016/a/a5/Toulouse_France_logoeel.jpg" style="width:30%"></center>
 +
</div>
 +
</div>
 
 
−
<p> Tuto digestion
+
<div class="descriptionArea2" id="Mexique2">
−
</p>
+
<br><br>
−
</div>
+
<div id=video style="margin-left:80px">  
−
+
<video width="490" height="275" controls>
−
<div class="descriptionArea2" id="Mexique1">
+
  <source src="https://static.igem.org/mediawiki/2016/a/a0/Toulouse_France_TutoLigation_span.mp4" type="video/mp4">
−
<div id=video style="margin-left:80px">  
+
Your browser does not support the video tag.
−
<video width="800" height="450" controls>
+
</video>
−
  <source src="https://static.igem.org/mediawiki/2016/d/d1/Toulouse_France_TutoDigestion_span.mp4" type="video/mp4">
+
<center><h3><a href="https://2016.igem.org/Team:Tec-Monterrey">Tec-Monterrey Team</h3></a></center>
−
Your browser does not support the video tag.
+
−
</video>
+
<center><img src="https://static.igem.org/mediawiki/2016/0/0e/Toulouse_France_logomonterey.jpg" style="width:30%"></center>
 +
</div>
 
</div>
 
</div>
  
−
<h3></h3>
+
<div class="descriptionArea2" id="France2">
−
<p>
+
<br><br>
 +
<div id=video style="margin-left:80px">
 +
<video width="490" height="275" controls>
 +
  <source src="https://static.igem.org/mediawiki/2016/7/7d/Toulouse_France_TutoLigation_french.mp4" type="video/mp4">
 +
Your browser does not support the video tag.
 +
</video>
 +
<center><h3><a href="https://2016.igem.org/Team:Bordeaux">Bordeaux Team</h3></a></center>
 +
 +
<center><img src="https://static.igem.org/mediawiki/2016/d/d0/Toulouse_France_logobdx.jpg" style="width:30%"></center>
 +
</div>
 +
</div>
 +
 +
<div class="descriptionArea2" id="Chine2">
 +
<br><br>
 +
<div id=video style="margin-left:80px">
 +
<video width="490" height="275" controls>
 +
  <source src="https://static.igem.org/mediawiki/2016/8/83/Toulouse_France_TutoLigation_chin.mp4" type="video/mp4">
 +
Your browser does not support the video tag.
 +
</video>
 +
<center><h3><a href="https://2016.igem.org/Team:BIT">BIT Team</h3></a></center>
 +
 +
<center><img src="https://static.igem.org/mediawiki/2016/9/96/Toulouse_France_logobit.jpg" style="width:30%"></center>
 +
 +
</div>
 +
</div>
  
−
</p>
+
<div class="descriptionArea2" id="German2">
−
</div>
+
<br><br>
−
+
<div id=video style="margin-left:80px">
−
</div>
+
<video width="490" height="275" controls>
 +
<source src="https://static.igem.org/mediawiki/2016/1/1c/Toulouse_France_TutoLigation_spanish.mp4" type="video/mp4">
 +
Your browser does not support the video tag.
 +
</video>
 +
<center><h3><a href="https://2016.igem.org/Team:Hamburg">Hamburg Team</h3></a></center>
 +
 +
<center><img src="https://static.igem.org/mediawiki/2016/d/d4/Toulouse_France_logomunich.jpg" style="width:30%"></center>
 +
 +
</div>
 +
</div>
 +
<div style="clear: both;"></div>
 +
</div>
 +
</div>
 
</div>
 
</div>
  
 +
<div class="column full_size" style="padding:20px 10%;">
 +
 +
<div class="textContent" style="width:90%;">
 +
<div class="column-left">
 +
 +
<div id="teamGallery2">
 +
<h2>PCR</h2>
 +
<p>PCR, or Polymerase Chain Reaction, is a technique that amplify a DNA sequence by using primers and DNA polymerase. Throughout this video, you will see how to perform a PCR. A theoretical explanation of the way how PCR works ends the video. </p>
 +
 +
 +
<div class="box">
 +
<div class="innerBox">
 +
<a href="https://2016.igem.org/Team:Toulouse_France/Collaborations#ImperialCollege3">
 +
<img src="https://static.igem.org/mediawiki/2016/9/98/Toulouse_France_Collaboration_GBflag.png" title="Imperial College3">
 +
</a>
 +
</div>
 +
</div>
  
−
+
<div class="box">
−
<div class="textContent" style="width:90%;">
+
<div class="innerBox">
−
<div class="column-left">
+
<a href="https://2016.igem.org/Team:Toulouse_France/Collaborations#Leiden3">
 +
<img src="https://static.igem.org/mediawiki/2016/a/ac/Toulouse_France_Collaboration_NDflag.png" title="Leiden3">
 +
</a>
 +
</div>
 +
</div>
 +
 +
<div class="box">
 +
<div class="innerBox">
 +
<a href="https://2016.igem.org/Team:Toulouse_France/Collaborations#Brazil3">
 +
<img src="https://static.igem.org/mediawiki/2016/5/58/Toulouse_France_brazilflag.jpg" title="Brazil3">
 +
</a>
 +
</div>
 +
</div>
  
−
<div id="teamGallery2">
+
<div class="box">
−
<h2>Ligation</h2>
+
<div class="innerBox">
−
+
<a href="https://2016.igem.org/Team:Toulouse_France/Collaborations#Mexique3">
−
<div class="box">
+
<img src="https://static.igem.org/mediawiki/2016/f/f3/Toulouse_France_Collaboration_Mexiqueflag.png" title="Mexique3">
−
<div class="innerBox">
+
</a>
−
<a href="https://2016.igem.org/Team:Toulouse_France/Collaborations#ImperialCollege2">
+
</div>
−
<img src="https://static.igem.org/mediawiki/2016/9/98/Toulouse_France_Collaboration_GBflag.png" title="Imperial College2">
+
−
</a>
+
 
</div>
 
</div>
−
</div>
+
 
 +
<div class="box">
 +
<div class="innerBox">
 +
<a href="https://2016.igem.org/Team:Toulouse_France/Collaborations#France3">
 +
<img src="https://static.igem.org/mediawiki/2016/f/f2/Toulouse_France_Collaboration_Franceflag.png" title="France3">
 +
</a>
 +
</div>
 +
</div>
 +
 
 +
<div class="box">
 +
<div class="innerBox">
 +
<a href="https://2016.igem.org/Team:Toulouse_France/Collaborations#Chine3">
 +
<img src="https://static.igem.org/mediawiki/2016/f/f4/Toulouse_France_Collaboration_Chinaflag.png" title="Chine3">
 +
</a>
 +
</div>
 +
</div>
 +
 
 +
<div class="box">
 +
<div class="innerBox">
 +
<a href="https://2016.igem.org/Team:Toulouse_France/Collaborations#German3">
 +
<img src="https://static.igem.org/mediawiki/2016/6/69/Toulouse_France_Collaboration_Allemagneflag.png" title="German3">
 +
</a>
 +
</div>
 +
</div>
 +
 +
</div>
 +
<div style="clear: both"></div>
 +
</div>
 
 
−
<div class="box">
+
<div class="column-right">
−
<div class="innerBox">
+
 
−
<a href="https://2016.igem.org/Team:Toulouse_France/Collaborations#Leiden2">
+
<div class="descriptionArea2" id="empty"></div>
−
<img src="https://static.igem.org/mediawiki/2016/a/ac/Toulouse_France_Collaboration_NDflag.png" title="Leiden2">
+
 
−
</a>
+
<div class="descriptionArea2" id="ImperialCollege3">
 +
<br><br>
 +
<div id=video style="margin-left:80px">  
 +
<video width="490" height="275" controls>
 +
  <source src="https://static.igem.org/mediawiki/2016/e/e9/Toulouse_France_TutoPCR_english.mp4" type="video/mp4">
 +
Your browser does not support the video tag.
 +
</video>
 +
<center><h3><a href="https://2016.igem.org/Team:Imperial_College">Imperial College of London team</a></h3></center>
 +
 
 +
<center><img src="https://static.igem.org/mediawiki/2016/7/7d/Toulouse_France_logoimperial.jpg" style="width:30%"></center>
 +
 +
</div>
 +
</div>
 +
 +
<div class="descriptionArea2" id="Leiden3">
 +
<br><br>
 +
<div id=video style="margin-left:80px">  
 +
<video width="490" height="275" controls>
 +
  <source src="https://static.igem.org/mediawiki/2016/7/70/Toulouse_France_TutoPCR_dutch.mp4" type="video/mp4">
 +
Your browser does not support the video tag.
 +
</video>
 +
<center><h3><a href="https://2016.igem.org/Team:Leiden">Leiden Team</h3></a></center>
 +
 +
<center><img src="https://static.igem.org/mediawiki/2016/3/3f/Toulouse_France_logoleiden.jpg" style="width:30%"></center>
 +
</div>
 +
</div>
 +
 +
<div class="descriptionArea2" id="Brazil3">
 +
<br><br>
 +
<div id=video style="margin-left:80px">
 +
<video width="490" height="275" controls>
 +
  <source src="https://static.igem.org/mediawiki/2016/6/6b/Toulouse_France_TutoPCR_portu.mp4" type="video/mp4">
 +
Your browser does not support the video tag.
 +
</video>
 +
<center><h3><a href="https://2016.igem.org/Team:USP-EEL-Brazil">EEL Brazil Team</h3></a></center>
 +
 +
<center><img src="https://static.igem.org/mediawiki/2016/a/a5/Toulouse_France_logoeel.jpg" style="width:30%"></center>
 +
 
</div>
 
</div>
 
</div>
 
</div>
 
 
−
<div class="box">
+
<div class="descriptionArea2" id="Mexique3">
−
<div class="innerBox">
+
<br><br>
−
<a href="https://2016.igem.org/Team:Toulouse_France/Collaborations#Portugal2">
+
<div id=video style="margin-left:80px">  
−
<img src="https://static.igem.org/mediawiki/2016/2/2a/Toulouse_France_Collaboration_Portugalflag.png" title="Portugal2">
+
−
</a>
+
<p>
 +
Will come soon
 +
<br><br>
 +
</p>
 +
 +
<center><h3><a href="https://2016.igem.org/Team:Tec-Monterrey">Tec-Monterrey Team</h3></a></center>
 +
 +
<center><img src="https://static.igem.org/mediawiki/2016/0/0e/Toulouse_France_logomonterey.jpg" style="width:30%"></center>
 
</div>
 
</div>
 +
</div>
 +
 +
<div class="descriptionArea2" id="France3">
 +
<br><br>
 +
<div id=video style="margin-left:80px">
 +
<video width="490" height="275" controls>
 +
  <source src="https://static.igem.org/mediawiki/2016/a/a9/Toulouse_France_TutoPCR_french.mp4" type="video/mp4">
 +
Your browser does not support the video tag.
 +
</video>
 +
<center><h3><a href="https://2016.igem.org/Team:Bordeaux">Bordeaux Team</h3></a></center>
 +
 +
<center><img src="https://static.igem.org/mediawiki/2016/d/d0/Toulouse_France_logobdx.jpg" style="width:30%"></center>
 +
</div>
 +
 
</div>
 
</div>
 
 
−
<div class="box">
+
<div class="descriptionArea2" id="Chine3">
−
<div class="innerBox">
+
<br><br>
−
<a href="https://2016.igem.org/Team:Toulouse_France/Collaborations#Mexique2">
+
<div id=video style="margin-left:80px">  
−
<img src="https://static.igem.org/mediawiki/2016/f/f3/Toulouse_France_Collaboration_Mexiqueflag.png" title="Mexique2">
+
<video width="490" height="275" controls>
−
</a>
+
  <source src="https://static.igem.org/mediawiki/2016/4/49/Toulouse_France_TutoPCR_chin.mp4" type="video/mp4">
 +
Your browser does not support the video tag.
 +
</video>
 +
<center><h3><a href="https://2016.igem.org/Team:BIT">BIT Team</h3></a></center>
 +
 +
<center><img src="https://static.igem.org/mediawiki/2016/9/96/Toulouse_France_logobit.jpg" style="width:30%"></center>
 
</div>
 
</div>
−
</div>
+
</div>
−
+
 
−
</div>
+
<div class="descriptionArea2" id="German3">
−
<div style="clear: both"></div>
+
<br><br>
 +
<div id=video style="margin-left:80px">
 +
 +
 +
<p>
 +
Will come soon
 +
<br><br>
 +
</p>
 +
 +
<center><h3><a href="https://2016.igem.org/Team:Hamburg">Hamburg Team</h3></a></center>
 +
 +
<center><img src="https://static.igem.org/mediawiki/2016/d/d4/Toulouse_France_logomunich.jpg" style="width:30%"></center>
 +
 +
</div>
 +
</div>
 +
 +
<div style="clear: both;"></div>
 +
 +
</div>
 
</div>
 
</div>
−
+
</div>
−
<div class="column-right">
+
  
−
<div class="descriptionArea2" id="empty"></div>
+
<div class="column full_size" style="padding:20px 10%;">
 +
 +
<div class="textContent" style="width:90%;">
 +
<div class="column-left">
 +
 +
<div id="teamGallery2">
 +
<h2>Transformation</h2>
 +
<p>A transformation is a method that allows to insert a plasmid in a <i> E. coli</i> bacteria. Several method to transform a bacteria exist, but we chose to use a thermal shock method for bacteria to become permeable. </p>
  
 +
<div class="box">
 +
<div class="innerBox">
 +
<a href="https://2016.igem.org/Team:Toulouse_France/Collaborations#ImperialCollege4">
 +
<img src="https://static.igem.org/mediawiki/2016/9/98/Toulouse_France_Collaboration_GBflag.png" title="Imperial College4">
 +
</a>
 +
</div>
 +
</div>
  
−
<div class="descriptionArea2" id="ImperialCollege2">
+
−
<div id=video style="margin-left:80px">  
+
<div class="box">
−
<video width="800" height="450" controls>
+
<div class="innerBox">
−
  <source src="https://static.igem.org/mediawiki/2016/4/47/Toulouse_France_TutoLigation_english.mp4" type="video/mp4">
+
<a href="https://2016.igem.org/Team:Toulouse_France/Collaborations#Leiden4">
−
Your browser does not support the video tag.
+
<img src="https://static.igem.org/mediawiki/2016/a/ac/Toulouse_France_Collaboration_NDflag.png" title="Leiden4">
−
</video>
+
</a>
−
</div>
+
</div>
−
+
</div>
−
<h3>tuto ligation</h3>
+
−
<p>
+
  
−
</p>
+
<div class="box">
−
</div>
+
<div class="innerBox">
 +
<a href="https://2016.igem.org/Team:Toulouse_France/Collaborations#Brazil4">
 +
<img src="https://static.igem.org/mediawiki/2016/5/58/Toulouse_France_brazilflag.jpg" title="Brazil4">
 +
</a>
 +
</div>
 +
</div>
  
−
<div class="descriptionArea2" id="Leiden2">
+
−
<div id=video style="margin-left:80px">  
+
<div class="box">
−
<video width="800" height="450" controls>
+
<div class="innerBox">
−
  <source src="https://static.igem.org/mediawiki/2016/d/d6/Toulouse_France_TutoLigation_dutch.mp4" type="video/mp4">
+
<a href="https://2016.igem.org/Team:Toulouse_France/Collaborations#Mexique4">
−
Your browser does not support the video tag.
+
<img src="https://static.igem.org/mediawiki/2016/f/f3/Toulouse_France_Collaboration_Mexiqueflag.png" title="Mexique4">
−
</video>
+
</a>
−
</div>
+
</div>
−
+
</div>
−
<h3></h3>
+
−
<p>
+
<div class="box">
−
</p>
+
<div class="innerBox">
−
</div>
+
<a href="https://2016.igem.org/Team:Toulouse_France/Collaborations#France4">
 +
<img src="https://static.igem.org/mediawiki/2016/f/f2/Toulouse_France_Collaboration_Franceflag.png" title="France4">
 +
</a>
 +
</div>
 +
</div>
  
−
<div class="descriptionArea2" id="Portugal2">
+
<div class="box">
−
<div id=video style="margin-left:80px">  
+
<div class="innerBox">
−
<video width="800" height="450" controls>
+
<a href="https://2016.igem.org/Team:Toulouse_France/Collaborations#Chine4">
−
  <source src="https://static.igem.org/mediawiki/2016/7/71/Toulouse_France_TutoLigation_portu.mp4" type="video/mp4">
+
<img src="https://static.igem.org/mediawiki/2016/f/f4/Toulouse_France_Collaboration_Chinaflag.png" title="Chine4">
−
Your browser does not support the video tag.
+
</a>
−
</video>
+
</div>
 +
</div>
 +
 +
<div class="box">
 +
<div class="innerBox">
 +
<a href="https://2016.igem.org/Team:Toulouse_France/Collaborations#German4">
 +
<img src="https://static.igem.org/mediawiki/2016/6/69/Toulouse_France_Collaboration_Allemagneflag.png" title="German4">
 +
</a>
 +
</div>
 +
</div>
 +
 
</div>
 
</div>
−
+
<div style="clear: both"></div>
−
<h3></h3>
+
−
<p>
+
  
−
</p>
 
 
</div>
 
</div>
 
 
−
<div class="descriptionArea2" id="Mexique2">
+
<div class="column-right">
−
<div id=video style="margin-left:80px">  
+
 
−
<video width="800" height="450" controls>
+
<div class="descriptionArea2" id="empty"></div>
−
  <source src="https://static.igem.org/mediawiki/2016/a/a0/Toulouse_France_TutoLigation_span.mp4" type="video/mp4">
+
−
Your browser does not support the video tag.
+
<div class="descriptionArea2" id="ImperialCollege4">
−
</video>
+
<br><br>
 +
<div id=video style="margin-left:80px">  
 +
<video width="490" height="275" controls>
 +
  <source src="https://static.igem.org/mediawiki/2016/4/49/Toulouse_France_TutoTransformation_english.mp4" type="video/mp4">
 +
Your browser does not support the video tag.
 +
</video>
 +
<center><h3><a href="https://2016.igem.org/Team:Imperial_College">Imperial College of London team</a></h3></center>
 +
 
 +
<center><img src="https://static.igem.org/mediawiki/2016/7/7d/Toulouse_France_logoimperial.jpg" style="width:30%"></center>
 +
 +
</div>
 +
 
</div>
 
</div>
 +
 +
<div class="descriptionArea2" id="Leiden4">
 +
<br><br>
 +
<div id=video style="margin-left:80px">
 +
<video width="490" height="275" controls>
 +
  <source src="https://static.igem.org/mediawiki/2016/f/f2/Toulouse_France_TutoTransformation_dutch.mp4" type="video/mp4">
 +
Your browser does not support the video tag.
 +
</video>
 +
<center><h3><a href="https://2016.igem.org/Team:Leiden">Leiden Team</h3></a></center>
 +
 +
<center><img src="https://static.igem.org/mediawiki/2016/3/3f/Toulouse_France_logoleiden.jpg" style="width:30%"></center>
 +
 +
</div>
  
−
<h3></h3>
+
</div>
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<p>
+
−
</p>
+
−
</div>
+
−
</div>
+
−
</div>
+
−
+
 
 
−
<div class="textContent" style="width:90%;">
+
<div class="descriptionArea2" id="Brazil4">
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<div class="column-left">
+
<br><br>
−
+
<div id=video style="margin-left:80px">  
−
<div id="teamGallery2">
+
<video width="490" height="275" controls>
−
<h2>PCR</h2>
+
<source src="https://static.igem.org/mediawiki/2016/3/37/Toulouse_France_TutoTransformation_portu.mp4" type="video/mp4">
−
+
Your browser does not support the video tag.
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<div class="box">
+
</video>
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<div class="innerBox">
+
<center><h3><a href="https://2016.igem.org/Team:USP-EEL-Brazil">EEL Brazil Team</h3></a></center>
−
<a href="https://2016.igem.org/Team:Toulouse_France/Collaborations#ImperialCollege3">
+
−
<img src="https://static.igem.org/mediawiki/2016/9/98/Toulouse_France_Collaboration_GBflag.png" title="Imperial College3">
+
<center><img src="https://static.igem.org/mediawiki/2016/a/a5/Toulouse_France_logoeel.jpg" style="width:30%"></center>
−
</a>
+
 
</div>
 
</div>
 
</div>
 
</div>
−
 
+
−
<div class="box">
+
<div class="descriptionArea2" id="Mexique4">
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<div class="innerBox">
+
<br><br>
−
<a href="https://2016.igem.org/Team:Toulouse_France/Collaborations#Leiden3">
+
<div id=video style="margin-left:80px">  
−
<img src="https://static.igem.org/mediawiki/2016/a/ac/Toulouse_France_Collaboration_NDflag.png" title="Leiden3">
+
<video width="490" height="275" controls>
−
</a>
+
  <source src="https://static.igem.org/mediawiki/2016/d/d1/Toulouse_France_TutoTransformation_span.mp4" type="video/mp4">
 +
Your browser does not support the video tag.
 +
</video>
 +
<center><h3><a href="https://2016.igem.org/Team:Tec-Monterrey">Tec-Monterrey Team</h3></a></center>
 +
 +
<center><img src="https://static.igem.org/mediawiki/2016/0/0e/Toulouse_France_logomonterey.jpg" style="width:30%"></center>
 +
 
</div>
 
</div>
−
</div>
+
</div>
 
 
−
<div class="box">
+
<div class="descriptionArea2" id="Chine4">
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<div class="innerBox">
+
<br><br>
−
<a href="https://2016.igem.org/Team:Toulouse_France/Collaborations#Portugal3">
+
<div id=video style="margin-left:80px">  
−
<img src="https://static.igem.org/mediawiki/2016/2/2a/Toulouse_France_Collaboration_Portugalflag.png" title="Portugal3">
+
<video width="490" height="275" controls>
−
</a>
+
<source src="https://static.igem.org/mediawiki/2016/d/d5/Toulouse_France_TutoTransformation_chin.mp4" type="video/mp4">
 +
Your browser does not support the video tag.
 +
</video>
 +
<center><h3><a href="https://2016.igem.org/Team:BIT">BIT Team</h3></a></center>
 +
 +
<center><img src="https://static.igem.org/mediawiki/2016/9/96/Toulouse_France_logobit.jpg" style="width:30%"></center>
 
</div>
 
</div>
−
</div>
+
</div>
  
−
<div class="box">
+
<div class="descriptionArea2" id="France4">
−
<div class="innerBox">
+
<br><br>
−
<a href="https://2016.igem.org/Team:Toulouse_France/Collaborations#Mexique3">
+
<div id=video style="margin-left:80px">  
−
<img src="https://static.igem.org/mediawiki/2016/f/f3/Toulouse_France_Collaboration_Mexiqueflag.png" title="Mexique3">
+
<video width="490" height="275" controls>
−
</a>
+
<source src="https://static.igem.org/mediawiki/2016/3/33/Toulouse_France_TutoTransformation_french.mp4" type="video/mp4">
 +
Your browser does not support the video tag.
 +
</video>
 +
<center><h3><a href="https://2016.igem.org/Team:Bordeaux">Bordeaux Team</h3></a></center>
 +
 +
<center><img src="https://static.igem.org/mediawiki/2016/d/d0/Toulouse_France_logobdx.jpg" style="width:30%"></center>
 
</div>
 
</div>
−
</div>
+
 
 +
</div>
 
 
 +
<div class="descriptionArea2" id="German4">
 +
<br><br>
 +
<div id=video style="margin-left:80px">
 +
<video width="490" height="275" controls>
 +
<source src="" type="video/mp4">
 +
Your browser does not support the video tag.
 +
</video>
 +
<center><h3><a href="https://2016.igem.org/Team:Hamburg">Hamburg Team</h3></a></center>
 +
 +
<center><img src="https://static.igem.org/mediawiki/2016/d/d4/Toulouse_France_logomunich.jpg" style="width:30%"></center>
 +
 +
</div>
 +
</div>
 +
 +
<div style="clear: both;"></div>
 +
 
</div>
 
</div>
−
<div style="clear: both"></div>
 
 
</div>
 
</div>
−
+
</div>
−
<div class="column-right">
+
  
−
<div class="descriptionArea2" id="empty"></div>
 
  
−
<div class="descriptionArea2" id="ImperialCollege3">
+
<br><br>
−
<div id=video style="margin-left:80px">  
+
 
−
<video width="800" height="450" controls>
+
 
−
  <source src="https://static.igem.org/mediawiki/2016/e/e9/Toulouse_France_TutoPCR_english.mp4" type="video/mp4">
+
 
−
Your browser does not support the video tag.
+
 
−
</video>
+
<div class="column full_size" style="text-align:justify; font-size:14px; color:black;">
−
</div>
+
<div style="padding-top:30px; padding-left: 10%; padding-right: 10%;">
−
+
<div class="hoc container clear">
−
<h3>c</h3>
+
<br><br>
−
<p>
+
<u><p class="title1" id="select1" style="text-align:center">Growth study of <i>B. subtilis</i> and <i>P. fluorescens</i></p></u>
 +
<p class="texteb">
 +
<center><img src="https://static.igem.org/mediawiki/2016/3/3e/Toulouse_France_erlenmeyers.jpg" style="margin-top:20px;"></center>
 +
<br><br>In exchange for recording the script in English, we studied the optimal growth of Bacillus subtilis and Pseudomonas fluorescens for the <a href="https://2016.igem.org/Team:Imperial_College">Imperial London College team</a>. First, we did an experimental plan to observe if pH is in interaction with temperature. Finally, the conclusion was that pH and temperature were two independent parameters. We ran the experiments again, changing only one parameter.
 
</p>
 
</p>
 +
<br><br>
 +
<p class="texteb">
 +
 +
<b style="font-size:16px;">Results</b><br><br>
 +
 +
<center><table style="border-width:1px; border-color:black; width:80%; border-spacing: 2px; background-color:#ccc; border-collapse: separate;">
 +
<tbody style="background-color:white;">
 +
 +
<!--1ere ligne-->
 +
<tr>
 +
<td style="background-color:#f8f8f8;"><p class ="texte" style="text-align:center; margin: 0 30px;"></td>
 +
<td style=" background-color:#f8f8f8;"><p class ="texte" style="text-align:center; margin: 0 10px;">Optimum Temperature (°C)</td>
 +
<td style=" background-color:#f8f8f8;"><p class ="texte" style="text-align:center; margin: 0 10px;">Optimum pH</td>
 +
<td style=" background-color:#f8f8f8;"><p class ="texte" style="text-align:center; margin: 0 10px;">Doubling time (minutes)</td>
 +
</tr>
 +
<!--2eme ligne-->
 +
<tr>
 +
<td style=" background-color:#f8f8f8"><p class ="texte" style="padding-top: 10px; text-align:center;"><i>Bacillus subtilis</i> (WT 168)</p></td>
 +
<td><p class ="texte" style="text-align:center">34-37</p></td>
 +
<td><p class ="texte" style="text-align:center">8</p>
 +
<td><p class ="texte" style="text-align:center">41</p></td>
 +
</tr>
 +
 +
<!--3eme ligne-->
 +
<tr>
 +
<td style=" background-color:#f8f8f8"><p class ="texte" style="padding-top: 10px; text-align:center;"><i>Pseudomonas fluorescens</i> (SBW25)</p></td>
 +
<td><p class ="texte" style="text-align:center"><29</p></td>
 +
<td><p class ="texte" style="text-align:center">7,5</p>
 +
<td><p class ="texte" style="text-align:center"><80</p>
 +
</tr>
 +
 +
</tbody>
 +
</table> </center>
 +
<br><br>Optimum temperatures and pH and the doubling times are summarized in the table for our two strains.
 +
</p>
 +
<center><img src="https://static.igem.org/mediawiki/2016/7/7d/Toulouse_France_logoimperial.jpg" style="width:15%"></center>
 
</div>
 
</div>
 +
</div>
 +
</div>
 +
 +
 +
<div class="column full_size"  style="text-align:justify; font-size:14px; color:black;">
 +
<div style="padding-top:30px; padding-left: 10%; padding-right: 10%;">
 +
<div class="hoc container clear">
 
 
−
<div class="descriptionArea2" id="Leiden3">
+
<u><p class="title1" id="select1" style="text-align:center">3D conception</p></u>
−
<div id=video style="margin-left:80px">  
+
<p class="texteb">
−
<video width="800" height="450" controls>
+
−
  <source src="https://static.igem.org/mediawiki/2016/7/70/Toulouse_France_TutoPCR_dutch.mp4" type="video/mp4">
+
We did a 3D Conception of the iGEM Bordeaux Team, on Blender.
−
Your browser does not support the video tag.
+
<br><br>
−
</video>
+
<video width="490" height="275" controls>
−
</div>
+
<source src="https://static.igem.org/mediawiki/2016/f/fa/Toulouse_France_logobordeau.mp4" type="video/mp4">
−
 
+
Your browser does not support the video tag.
−
<h3></h3>
+
</video>
−
<p>
+
 +
<br><br>We also did a 3D conception of their laboratory benching.
 +
 
 
 
</p>
 
</p>
−
</div>
+
</div>
 +
</div>
 +
</div>
 +
 
 +
 
 +
<div class="column full_size"  style="text-align:justify; font-size:14px; color:black;">
 +
<div style="padding-top:30px; padding-left: 10%; padding-right: 10%;">
 +
<div class="hoc container clear">
 
 
−
<div class="descriptionArea2" id="Portugal3">
+
<u><p class="title1" id="select1" style="text-align:center">Model of the plasmid loss</p></u>
−
<div id=video style="margin-left:80px">  
+
<p class="texteb">
−
<video width="800" height="450" controls>
+
−
  <source src="https://static.igem.org/mediawiki/2016/6/6b/Toulouse_France_TutoPCR_portu.mp4" type="video/mp4">
+
−
Your browser does not support the video tag.
+
−
</video>
+
−
</div>
+
  
−
<h3></h3>
+
We want to thank <a href="https://2016.igem.org/Team:Aix-Marseille">Aix-Marseille team</a> for the great help they give to us.
−
<p>
+
The aim of the model they conceived for us is to assess the evolution of plasmids throughout the culture,
 +
to determine which parameters can matter in the loss of those plasmids,
 +
and to precise what are the probabilities for a bacteria to loose its plasmids during cell division.
 +
As a plasmids can be a disadvantage for growth (energy spent into replicating processes) or a advantage
 +
(protection against a toxin) this question is hard to answer.
 +
But in this situation, where one type of plasmid can influence on the presence of the other type of plasmid in (and reciprocally)
 +
in the same bacteria, the question become too tough to answer and only a mathematical model can resolve such a interrogation!
 +
 
 +
<center>
 +
<a class="button-home" href="https://2016.igem.org/Team:Aix-Marseille/Model" style="border: 1px solid #282828;-webkit-border-radius: 5px;-moz-border-radius: 5px;border-radius: 5px;
 +
padding: 15px 15px; color: black; text-decoration: none; font-size: 18px; background: none; display: block; width: 500px; background-color: #8B0000">Click here to see the model they developped</a>
 +
</center>
 
</p>
 
</p>
 +
<center><img src="https://static.igem.org/mediawiki/2016/7/74/T--Aix-Marseille--logo-igem-amu-opti.svg" style="width:30%"></center>
 
</div>
 
</div>
−
+
</div>
−
<div class="descriptionArea2" id="Mexique3">
+
</div>
−
<div id=video style="margin-left:80px">
+
−
<video width="800" height="450" controls>
+
−
  <source src="" type="video/mp4">
+
−
Your browser does not support the video tag.
+
−
</video>
+
−
</div>
+
  
−
<h3></h3>
+
−
<p>
+
−
+
 +
<div class="column full_size"  style="text-align:justify; font-size:14px; color:black;">
 +
<div style="padding-top:30px; padding-left: 10%; padding-right: 10%;">
 +
<div class="hoc container clear">
 +
 +
<u><p class="title1" id="select1" style="text-align:center">Receipt of plasmids</p></u>
 +
<p class="texteb">
 +
We want to thank the 2012 <a href="https://2012.igem.org/Team:LMU-Munich">Munich team</a> for sending us 4 different shuttle plasmids. One of these, pBs0k-p, was very useful because we used it for cloning in E.coli and Bacillus subtilis.
 
</p>
 
</p>
−
</div>
+
<center><img src="" style="width:15%"></center>
 +
</div>
 +
</div>
 +
</div>
 +
 +
 +
<div class="column full_size"  style="text-align:justify; font-size:14px; color:black;">
 +
<div style="padding-top:30px; padding-left: 10%; padding-right: 10%;">
 +
<div class="hoc container clear">
 
 
−
</div>
+
<u><p class="title1" id="select1" style="text-align:center">Mailing of kit</p></u>
 +
<p class="texteb">
 +
2016 <a href="https://2016.igem.org/Team:Evry">Evry team</a> asked us to send a Ludox solution that was in our iGEM Kit. They stored their own solution at -20°C so it was not usable anymore. But it was important for them to participate to the InterLab project so we helped them.
 +
</p>
 +
<center><img src="https://static.igem.org/mediawiki/2016/8/88/Toulouse_France_logoevry.jpg" style="width:15%"></center>
 +
</div>
 +
</div>
 
</div>
 
</div>
 
 
−
<div class="textContent" style="width:90%;">
+
−
<div class="column-left">
+
<div class="column full_size" style="text-align:justify; font-size:14px; color:black;">
 +
<div style="padding-top:30px; padding-left: 10%; padding-right: 10%;">
 +
<div class="hoc container clear">
 
 
−
<div id="teamGallery2">
+
<u><p class="title1" id="select1" style="text-align:center">Protocol translation</p></u>
−
<h2>Transformation</h2>
+
<p class="texteb">
 +
2016 <a href="https://2016.igem.org/Team:METU_HS_Ankara">METU HS Ankara</a> team wanted to create a database, which consists of different language protocols. They asked us to translate their protocol in French.
 +
</p>
 
 
−
<div class="box">
+
<center><img src="https://static.igem.org/mediawiki/2016/7/70/Toulouse_France_logoankara.jpg" style="width:15%"></center>
−
<div class="innerBox">
+
−
<a href="https://2016.igem.org/Team:Toulouse_France/Collaborations#ImperialCollege4">
+
−
<img src="https://static.igem.org/mediawiki/2016/9/98/Toulouse_France_Collaboration_GBflag.png" title="Imperial College4">
+
−
</a>
+
−
</div>
+
−
</div>
+
−
 
+
−
+
−
<div class="box">
+
−
<div class="innerBox">
+
−
<a href="https://2016.igem.org/Team:Toulouse_France/Collaborations#Leiden4">
+
−
<img src="https://static.igem.org/mediawiki/2016/a/ac/Toulouse_France_Collaboration_NDflag.png" title="Leiden4">
+
−
</a>
+
−
</div>
+
−
</div>
+
−
 
+
−
<div class="box">
+
−
<div class="innerBox">
+
−
<a href="https://2016.igem.org/Team:Toulouse_France/Collaborations#Portugal44">
+
−
<img src="https://static.igem.org/mediawiki/2016/2/2a/Toulouse_France_Collaboration_Portugalflag.png" title="Portugal4">
+
−
</a>
+
−
</div>
+
−
</div>
+
−
 
+
−
+
−
<div class="box">
+
−
<div class="innerBox">
+
−
<a href="https://2016.igem.org/Team:Toulouse_France/Collaborations#Mexique4">
+
−
<img src="https://static.igem.org/mediawiki/2016/f/f3/Toulouse_France_Collaboration_Mexiqueflag.png" title="Mexique4">
+
−
</a>
+
−
</div>
+
−
</div>
+
 
</div>
 
</div>
−
<div style="clear: both"></div>
 
−
 
 
</div>
 
</div>
−
<div class="column-right">
+
</div>
−
 
+
−
<div class="descriptionArea2" id="empty"></div>
+
 
 
−
<div class="descriptionArea2" id="ImperialCollege4">
+
−
<div id=video style="margin-left:80px">  
+
<div class="column full_size" style="text-align:justify; font-size:14px; color:black;">
−
<video width="800" height="450" controls>
+
<div style="padding-top:30px; padding-left: 10%; padding-right: 10%;">
−
  <source src="https://static.igem.org/mediawiki/2016/4/49/Toulouse_France_TutoTransformation_english.mp4" type="video/mp4">
+
<div class="hoc container clear">
−
Your browser does not support the video tag.
+
−
</video>
+
<u><p class="title1" id="select1" style="text-align:center">Surveys</p></u>
−
</div>
+
<p class="texteb">
−
+
We filled in different surveys all along the year. Among them, surveys for:
−
<h3></h3>
+
<ul>
−
<p>
+
<li><a href="https://2016.igem.org/Team:Bordeaux">Bordeaux</a></li>
 +
<li><a href="https://2016.igem.org/Team:Ionis_Paris">IONIS</a></li>
 +
<li><a href="https://2016.igem.org/Team:Evry">Evry</a></li>
 +
<li><a href="https://2016.igem.org/Team:Valencia_UPV">Valencia</a></li>
 +
<li><a href="https://2016.igem.org/Team:TecCEM">TecCEM Collegiate</a></li>
 +
<li><a href="https://2016.igem.org/Team:Purdue">Purdue</a></li>
 +
<li><a href="">UBGM</a></li>
 +
</ul>
 
</p>
 
</p>
 
</div>
 
</div>
 +
</div>
 +
</div>
 +
 +
 +
<div>
 +
 +
<p><br><br><br><br></p>
 +
 +
 +
</div>
 +
 +
 +
<div class="column full_size" style="padding:20px 10%;">
 +
<div class="textContent">
 +
<div class="column-left">
 +
 +
<div id="teamGallery">
 +
 +
<h2>Meet Ups</h2>
 
 
−
<div class="descriptionArea2" id="Leiden4">
 
−
<div id=video style="margin-left:80px">
 
−
<video width="800" height="450" controls>
 
−
  <source src="https://static.igem.org/mediawiki/2016/f/f2/Toulouse_France_TutoTransformation_dutch.mp4" type="video/mp4">
 
−
Your browser does not support the video tag.
 
−
</video>
 
 
</div>
 
</div>
 +
</div>
 +
</div>
 +
 +
<br><br>
 +
  
−
<h3></h3>
+
<p style="font-size:15px;">
−
<p>
+
<br>
 +
<br>
 +
<b style="font-size:18px">The European Experience</b>
 +
<br>
 +
<br>
 +
The Evry and Ionis teams organized on the 1st and 2nd of July, a <b>huge meeting that gathered
  
−
</p>
+
more than 11 European teams</b>. In different rooms, each team had a space to hang its poster
−
</div>
+
−
+
−
<div class="descriptionArea2" id="Portugal4">
+
−
<div id=video style="margin-left:80px">
+
−
<video width="800" height="450" controls>
+
−
  <source src="https://static.igem.org/mediawiki/2016/3/37/Toulouse_France_TutoTransformation_portu.mp4" type="video/mp4">
+
−
Your browser does not support the video tag.
+
−
</video>
+
−
</div>
+
  
−
<h3></h3>
+
and set up flyers and communication supports. We explained our project and discussed with
−
<p>
+
−
</p>
+
−
</div>
+
−
+
−
<div class="descriptionArea2" id="Mexique4">
+
−
<div id=video style="margin-left:80px">
+
−
<video width="800" height="450" controls>
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other iGEM members. Some helped us on different aspects like modeling and confinement
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more about other brilliant projects. We met a lot of nice people from different teams and
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We thank very much the organizing teams for these amazing days in Paris! It was for us an
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One day, on our Facebook page, we received a message from Itzel Guzman, iGEM TEC
  
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the iGEM Toulouse team. We discussed a long time on our project over a drink and we
  
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&#127968;
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INSA Toulouse<br>
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135, Avenue de Rangueil<br>
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31077 TOULOUSE Cedex 4<br>
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France<br>         
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Latest revision as of 02:53, 20 October 2016

iGEM Toulouse 2016

Collaborations and Meet Up


Our Collaborations

Protocol videos

Our team decided to create protocol videos in order to help young scientists to manipulate. When people are working on Genetics engineering, there are several basic experiments that have to be controlled. During our experiments, we filmed 4 steps of cloning: PCR, digestion, ligation and transformation.
Watching it, people could see how to manipulate safely. We asked other igem teams to translate protocols in their mother tongue. We obtained protocol videos in English with Imperial College team, in Spanish thanks to the TEC-Monterrey team, in Dutch with Leiden team, in Chinese with BIT team, in Portuguese thanks to EEL team, in German with Hamburg team and in French with the collaboration of the Bordeaux team. We wrote the entire script and we added some guidance in red (minutes for each paragraphs for instance). Teams had to translate and record the script. Now, we possess a lot of protocols explained in six different languages, accessible to everybody on Youtube and on our wiki.

Digestion

This video presents the protocol to do enzymatic digestion of a DNA sample. This technique is useful for usual clonings.



Imperial College of London team



Leiden Team



EEL Brazil Team



Tec-Monterrey Team



Bordeaux Team



BIT Team



Hamburg Team

Ligation

When a DNA fragment is digested with digestion enzymes, it is possible to ligate its extremities with another digested fragment, if the extremities are cohesives. This method can be used to add an insert in a vector or a backbone.



Imperial College of London team



Leiden Team



EEL Brazil Team



Tec-Monterrey Team



Bordeaux Team



BIT Team



Hamburg Team

PCR

PCR, or Polymerase Chain Reaction, is a technique that amplify a DNA sequence by using primers and DNA polymerase. Throughout this video, you will see how to perform a PCR. A theoretical explanation of the way how PCR works ends the video.



Imperial College of London team



Leiden Team



EEL Brazil Team



Will come soon

Tec-Monterrey Team



Bordeaux Team



BIT Team



Will come soon

Hamburg Team

Transformation

A transformation is a method that allows to insert a plasmid in a E. coli bacteria. Several method to transform a bacteria exist, but we chose to use a thermal shock method for bacteria to become permeable.



Imperial College of London team



Leiden Team



EEL Brazil Team



Tec-Monterrey Team



BIT Team



Bordeaux Team



Hamburg Team





Growth study of B. subtilis and P. fluorescens



In exchange for recording the script in English, we studied the optimal growth of Bacillus subtilis and Pseudomonas fluorescens for the Imperial London College team. First, we did an experimental plan to observe if pH is in interaction with temperature. Finally, the conclusion was that pH and temperature were two independent parameters. We ran the experiments again, changing only one parameter.



Results

Optimum Temperature (°C)

Optimum pH

Doubling time (minutes)

Bacillus subtilis (WT 168)

34-37

8

41

Pseudomonas fluorescens (SBW25)

<29

7,5

<80


Optimum temperatures and pH and the doubling times are summarized in the table for our two strains.

3D conception

We did a 3D Conception of the iGEM Bordeaux Team, on Blender.



We also did a 3D conception of their laboratory benching.

Model of the plasmid loss

We want to thank Aix-Marseille team for the great help they give to us. The aim of the model they conceived for us is to assess the evolution of plasmids throughout the culture, to determine which parameters can matter in the loss of those plasmids, and to precise what are the probabilities for a bacteria to loose its plasmids during cell division. As a plasmids can be a disadvantage for growth (energy spent into replicating processes) or a advantage (protection against a toxin) this question is hard to answer. But in this situation, where one type of plasmid can influence on the presence of the other type of plasmid in (and reciprocally) in the same bacteria, the question become too tough to answer and only a mathematical model can resolve such a interrogation!

Click here to see the model they developped

Receipt of plasmids

We want to thank the 2012 Munich team for sending us 4 different shuttle plasmids. One of these, pBs0k-p, was very useful because we used it for cloning in E.coli and Bacillus subtilis.

Mailing of kit

2016 Evry team asked us to send a Ludox solution that was in our iGEM Kit. They stored their own solution at -20°C so it was not usable anymore. But it was important for them to participate to the InterLab project so we helped them.

Protocol translation

2016 METU HS Ankara team wanted to create a database, which consists of different language protocols. They asked us to translate their protocol in French.

Surveys

We filled in different surveys all along the year. Among them, surveys for:





Meet Ups





The European Experience

The Evry and Ionis teams organized on the 1st and 2nd of July, a huge meeting that gathered more than 11 European teams. In different rooms, each team had a space to hang its poster and set up flyers and communication supports. We explained our project and discussed with other iGEM members. Some helped us on different aspects like modeling and confinement of our bacteria. When we were not on our stand, we walked around the rooms and learnt more about other brilliant projects. We met a lot of nice people from different teams and we sympathized a lot with every French team.

The organizers arranged two lectures during the weekend. First it dealt with the challenges and the risks of synthetic biology and the second talked about a new economic world. Different people, like CEOs of company, trainees, young workers, or research scientists animated these round tables. One of our best memories was to meet the founder of iGEM: Randy Rettberg. It was an honor for us to speak with this person and to explain our project.

We thank very much the organizing teams for these amazing days in Paris! It was for us an enjoyable experience of what the Giant Jamboree will be in October.


Meeting with Itzel from TEC Monterrey team

One day, on our Facebook page, we received a message from Itzel Guzman, iGEM TEC Monterrey team member. She was in Toulouse for a semester and she asked us to meet us, the iGEM Toulouse team. We discussed a long time on our project over a drink and we sympathized a lot! We decided to collaborate together on the protocol videos (view more).





Contacts
    🏠 INSA Toulouse
    135, Avenue de Rangueil
    31077 TOULOUSE Cedex 4
    France
  • ☎ +00 (33) 6 13 64 89 40
  • ✉ igemtoulouse2016@gmail.com