Difference between revisions of "Team:Toulouse France/Collaborations"

Protocol videos

Our team decided to create protocol videos in order to help young scientists to manipulate. When people are working on Genetics engineering, there are several basic experiments that have to be controlled. During our experiments, we filmed 4 steps of cloning: PCR, digestion, ligation and transformation.
Watching it, people could see how to manipulate safely. We asked other igem teams to translate protocols in their mother tongue. We obtained protocol videos in English with Imperial College team, in Spanish thanks to the TEC-Monterrey team, in Dutch with Leiden team, in Chinese with BIT team, in Portuguese thanks to EEL team, in German with Hamburg team and in French with the collaboration of the Bordeaux team. We wrote the entire script and we added some guidance in red (minutes for each paragraphs for instance). Teams had to translate and record the script. Now, we possess a lot of protocols explained in six different languages, accessible to everybody on Youtube and on our wiki.

Growth study of B. subtilis and P. fluorescens

In exchange for recording the script in English, we studied the optimal growth of Bacillus subtilis and Pseudomonas fluorescens for the Imperial London College team. First, we did an experimental plan to observe if pH is in interaction with temperature. Finally, the conclusion was that pH and temperature were two independent parameters. We ran the experiments again, changing only one parameter.

Results

	Optimum Temperature (°C)	Optimum pH	Doubling time (minutes)
Bacillus subtilis (WT 168)	34-37	8	41
Pseudomonas fluorescens (SBW25)	<29	7,5	<80

Optimum temperatures and pH and the doubling times are summarized in the table for our two strains.

3D conception

We did a 3D Conception of the iGEM Bordeaux Team, on Blender.



We also did a 3D conception of their laboratory benching.

Model of the plasmid loss

We want to thank Aix-Marseille team for the great help they give to us. The aim of the model they conceived for us is to assess the evolution of plasmids throughout the culture, to determine which parameters can matter in the loss of those plasmids, and to precise what are the probabilities for a bacteria to loose its plasmids during cell division. As a plasmids can be a disadvantage for growth (energy spent into replicating processes) or a advantage (protection against a toxin) this question is hard to answer. But in this situation, where one type of plasmid can influence on the presence of the other type of plasmid in (and reciprocally) in the same bacteria, the question become too tough to answer and only a mathematical model can resolve such a interrogation!

Click here to see the model they developped

Receipt of plasmids

We want to thank the 2012 Munich team for sending us 4 different shuttle plasmids. One of these, pBs0k-p, was very useful because we used it for cloning in E.coli and Bacillus subtilis.

Mailing of kit

2016 Evry team asked us to send a Ludox solution that was in our iGEM Kit. They stored their own solution at -20°C so it was not usable anymore. But it was important for them to participate to the InterLab project so we helped them.

Protocol translation

2016 METU HS Ankara team wanted to create a database, which consists of different language protocols. They asked us to translate their protocol in French.

Surveys

We filled in different surveys all along the year. Among them, surveys for:

• Bordeaux
• IONIS
• Evry
• Valencia
• TecCEM Collegiate
• Purdue
• UBGM