Difference between revisions of "Team:SUSTech Shenzhen"

 
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                <h2 class="margin-top-0 text-primary">Discovering the Secret of Sound</h2>
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                    <p>We use synthetic biology methods to explore how cells sense mechanical stress, such as sound wave. Traditional gene expression regulation techniques, for example, optogenetics and chemical genetics, are commonly used in biological science but with certain limitations. Here we design a new way, audiogenetics, by which we can not only regulate gene expression in cells precisely and nontoxically, but also have an in-depth and comprehensive understanding of our feelings.</p>
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Audiogenetics is a useful tool for high-efficiency cell regulation. Compared to chemical genetics, it stimulates cells with better precision and less toxicity. Furthermore, signals are conveyed to target cells with little delay, leading to a shorter response time. To achieve our goal, membrane mechanosensitive channels (TRPC5 and Piezo) are chosen as receptors. Fluorescent calcium indicator (R-GECO) is employed to indicate cytoplasmic calcium level. Nuclear factor of activated T cells (NFAT) and YFP are used as downstream indicators to quantify the regulatory abilities. Microfluidic channels are also utilized in the pre-study in which shear stress, similar to sound waves, is applied on the cell surface as signal input to explore the basic parameters. A sound generator is constructed to test whether sound can trigger the channels as expected. Additionally, we use directed evolution to improve channels' selectivity for specific sound frequency and their sensitivity to sound of lower intensity.
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                    <h3>One Target</h3>
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                    <p class="text-muted">to make the world audible again</p>
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                    <h3>Two Channels</h3>
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                    <p class="text-muted">quantified to the core</p>
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                    <h3>Three Systems</h3>
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                    <p class="text-muted">made by scientists for scientists</p>
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Latest revision as of 02:56, 20 October 2016

Team SUSTC-Shenzhen

GLANCE
EXPLORE

In a Glance

One Target

to make the world audible again

Two Channels

quantified to the core

Three Systems

made by scientists for scientists

EXPLORE

Made by from the iGEM team SUSTech_Shenzhen.

Licensed under CC BY 4.0.

  • ©2016 SUSTech_Shenzhen