Difference between revisions of "Team:Tuebingen/Parts"

 
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<html>
 
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<script type='text/javascript'>
 
<script type='text/javascript'>
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$('#sectionProcedures > a').addClass('linkActive');
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$('#sectionLab > a').addClass('linkActive');
 
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</script>
  
 
<div class="contentArea">
 
<div class="contentArea">
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    <div class='contentBothCols'>
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<img id="Scribble" src="https://static.igem.org/mediawiki/2016/f/f3/T--Tuebingen--R2D2.png">
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        <p>
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<div class="contentRow">
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         <a href="https://2016.igem.org/Team:Tuebingen/Basic_Part">  
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        <div class="contentCell contentNarrowCell">
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          Here</a>
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            <p>Phosphofructokinase 1 </p>
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         <a href="https://2016.igem.org/Team:Tuebingen/Composite_Part">  
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         </div>
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           is </a>
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      <div class="contentCell contentMirijamsRaumZumAtmen"></div>
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         <a href="https://2016.igem.org/Team:Tuebingen/Part_Collection">
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<div class="contentCell">
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           a </a>
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<p> This part contains a 6-phosphofructoskinase from <em>Lactobacillus johnsonii</em> ATCC33200 that metabolizes fructose-6-phosphate to fructose-1,6-bisphosphate. This part is RFC 10 compatible. The part's codon usage is optimized for expression in lactobacilli. The codon usage of this part was optimized to balance DNA synthesis and expression in <em>L. Johnsonii</em>. Therefore, longterm and shortterm repeats had to be modified and biobrick restriction sites were removed. The part was modified for RFC10 at the prefix and suffix of the part.
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         <a href="https://2016.igem.org/Team:Tuebingen/Description">
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<p>(<a href='http://parts.igem.org/Part:BBa_K1882005'>BBa_K1882005</a>)</p>
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          small </a>
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</div>
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        demonstration for a block text.
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</div>
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<div class="contentRow">
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        <div class="contentCell contentNarrowCell">
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            <p> LDH-Promotor Phosphofructokinase </p>
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         </div>
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         amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut
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      <div class="contentCell contentMirijamsRaumZumAtmen"></div>
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<div class="contentCell">
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<p> This part encodes BBa_K1882002 and BBa_K1882000 resulting in a <em>L. johnsonii</em> ATCC33200 optimized phosphofructokinase under the control of a constitutive LDH-promotor. This leads to overexpression of the phosphofructokinase in <em>L. johnsonii</em> ATCC33200 when transferring this part into a lactobacillus expression vector.
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<p>(<a href='http://parts.igem.org/Part:BBa_K1882001'>BBa_K1882001</a>)</p>
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</div>
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         magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo
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</div>
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<div class="contentRow">
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           <div class="contentCell contentNarrowCell">
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            <p> LDH-Promotor </p>
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         </p>
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         </div>
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         <figure>
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      <div class="contentCell contentMirijamsRaumZumAtmen"></div>
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            <img src='../wiki/images/9/9b/Harry.jpg'' />
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<div class="contentCell">
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            <figcaption>
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<p> This part contains a the promoter of the <em>L. johnsonii</em> ATCC33200 lactate dehydrogenase, a constitutively expressed protein. This part is RFC10 compatible
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                Take a look at this awesome illustration!
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<p>(<a href='http://parts.igem.org/Part:BBa_K1882002'>BBa_K1882002</a>)</p>
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             </figcaption>
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</div>
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         </figure>
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</div>
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        <p>
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<div class="contentRow">
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            And it still keeps going!
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           <div class="contentCell contentNarrowCell">
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        </p>
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            <p> Fructose Transporter </p>
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    </div>
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         </div>
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      <div class="contentCell contentMirijamsRaumZumAtmen"></div>
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    <div class='contentRow'>
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<div class="contentCell">
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         <div class='contentCell contentSmallCell'>
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<p> This part encodes a carbohydrate transporter from <em>L. johnsonii</em> ATCC 33200 that imports fructose into the target cell. In this process, fructose is phosphorylated by an N-phosphohistidine residue to yield fructose-6-phosphate and histidine. The protein is part of the ABC transporter superfamily. This part ist RFC 10 compatible. The part's codon usage is optimized for expression in lactobacilli. The part was optimized to balance DNA synthesis by IDT and expression in <em>L. johnsonii</em>. Therefore longterm and shortterm repeats had to modified and biobrick restriction sites were removed.The part was modified for RFC10 at the prefix and suffix of the part.
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             <figure>
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<p>(<a href='http://parts.igem.org/Part:BBa_K1882006'>BBa_K1882006</a>)</p>
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                <img src='../wiki/images/9/9b/Harry.jpg' />
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</div>
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                <figcaption>
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</div>
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                    -- Some wizzard dude.
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<div class="contentRow">
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                </figcaption>
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         <div class="contentCell contentNarrowCell">
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            </figure>
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            <p> LDH-Promotor </p>
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            <p>
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         </div>
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      <div class="contentCell contentMirijamsRaumZumAtmen"></div>
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<div class="contentCell">
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<p> This part contains a the promoter of the <em>L. johnsonii</em> ATCC33200 lactate dehydrogenase, a constitutively expressed protein. This part is RFC10 compatible
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<p>(<a href='http://parts.igem.org/Part:BBa_K1882007'>BBa_K1882007</a>)</p>
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             </p>
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</div>
 +
</div>
 +
<div class="contentRow">
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         <div class="contentCell contentNarrowCell">
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            <p> Ketohexokinase </p>
 +
         </div>
 +
      <div class="contentCell contentMirijamsRaumZumAtmen"></div>
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<div class="contentCell">
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<p> This part encodes a ketohexokinase (ATP:D-fructose 1-phosphotransferase) from <em>Rattus norvegicus</em> optimized for expression in <em>Saccharomyces cerevisiae</em>.  The ketohexokinase converts fructose to fructose-1-phosphate using ATP to perform the phosphorylation. This protein is not common in yeast and  functions as toxin of the cell by trapping phosphate and therefore ATP in fructose-1-phosphate, not being able to be further metabolized. This result stop of growth of transformed yeast in fructose containing media (Donaldson et al., 1993). This part is RFC25 and RFC10 compatible.
 +
<p>(<a href='http://parts.igem.org/Part:BBa_K1882000'>BBa_K1882000</a>)</p>
 +
</div>
 +
</div>
 +
<div class="contentRow">
 +
         <div class="contentCell contentNarrowCell">
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            <p> ApaI-constPromotor </p>
 +
         </div>
 +
      <div class="contentCell contentMirijamsRaumZumAtmen"></div>
 +
<div class="contentCell">
 +
<p> This part encodes for BBa_J23102 with an ApaI restriction site upstream of it. Combination with BBa_K1882010 enables this part to clone a construct in a RFC compatible plasmid.
 +
<p>(<a href='http://parts.igem.org/Part:BBa_K1882009'>BBa_K1882009</a>)</p>
 +
</div>
 +
</div>
 +
<div class="contentRow">
 +
         <div class="contentCell contentNarrowCell">
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            <p> Terminator-Bam-Hind </p>
 +
         </div>
 +
      <div class="contentCell contentMirijamsRaumZumAtmen"></div>
 +
<div class="contentCell">
 +
<p> This part encodes for the rrnB T1 Terminator (BBa_B0010)  followed by a T7 Terminator (BBa_B0012) and BamHI and HindIII restriction sites. Combination with BBa_K1882009 enables this part to clone a construct in an RFC compatible plasmid.  
 +
<p>(<a href='http://parts.igem.org/Part:BBa_K1882010'>BBa_K1882010</a>)</p>
 +
</div>
 +
</div>
 +
<div class="contentRow">
 +
         <div class="contentCell contentNarrowCell">
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            <p> RBS-PezT </p>
 +
         </div>
 +
      <div class="contentCell contentMirijamsRaumZumAtmen"></div>
 +
<div class="contentCell">
 +
<p> This part encodes for a  codon optimized version of UDP-N-acetylglucosamine kinase (PezT toxin). Upon expression the toxin produces UDP-N-acetyl-alpha-D-glucosamine 3'-phosphate which inhibits MurA and therefore cell wall synthesis in <em>E. coli</em> as well as <em>L. johnsonii</em>. Besides the sequence for the PezT toxin this part contains an upstream located  ribosome binding site and could therefore be cloned direct behind any promoter of choice.
 +
<p>(<a href='http://parts.igem.org/Part:BBa_K1882011'>BBa_K1882011</a>)</p>
 +
</div>
 +
</div>
 +
<div class="contentRow">
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        <div class="contentCell contentNarrowCell">
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             <p> ZF ZFN-L </p>
 +
         </div>
 +
      <div class="contentCell contentMirijamsRaumZumAtmen"></div>
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<div class="contentCell">
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<p> This part contains the zinc finger ZF ZFN-L, see also Lombardo et al., 2007. The part is compatible with RFC10.
 +
<p>(<a href='http://parts.igem.org/Part:BBa_K1882012'>BBa_K1882012</a>)</p>
 +
</div>
 +
</div>
 +
<div class="contentRow">
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         <div class="contentCell contentNarrowCell">
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             <p> ZF K230R </p>
 +
        </div>
 +
      <div class="contentCell contentMirijamsRaumZumAtmen"></div>
 +
<div class="contentCell">
 +
<p> This part contains the zinc finger ZFK230R, see also Lee et al., 2010. The part is compatible with RFC10.
 +
<p>(<a href='http://parts.igem.org/Part:BBa_K1882013'>BBa_K1882013</a>)</p>
 +
</div>
 +
</div>
 +
<div class="contentRow">
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        <div class="contentCell contentNarrowCell">
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            <p> ZF-C7C7 </p>
 +
        </div>
 +
      <div class="contentCell contentMirijamsRaumZumAtmen"></div>  
 +
<div class="contentCell">
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<p> This part contains the zinc finger ZF-C7C7, see also Liu et al., 1997. The part is compatible with RFC10.
 +
<p>(<a href='http://parts.igem.org/Part:BBa_K1882014'>BBa_K1882014</a>)</p>
 +
</div>
 +
</div>
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<div class="contentRow">
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        <div class="contentCell contentNarrowCell">
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             <p> Kill 1 </p>
 
         </div>
 
         </div>
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        <div class='contentCell'>
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<div class="contentCell">
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<p> This part is a composite part of the following components: Apa-pRha + RBS-PezT + Terminator-Bam-Hind. It is RFC10 compatible, and can be used for negative selection.  
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<p>(<a href='http://parts.igem.org/Part:BBa_K1882015'>BBa_K1882015</a>)</p>
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</div>
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</div>
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<div class="contentRow">
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        <div class="contentCell contentNarrowCell">
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             <p> Apa-Bam-Promotor-ZFNLbind-RBS </p>
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            </p>
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             </p>
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         </div>
 
         </div>
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    </div>
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      <div class="contentCell contentMirijamsRaumZumAtmen"></div>  
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    <div class='contentRow'>
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<p>composite part of BBa_J23110; BBa_B0032
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            <p>
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<p>(<a href='http://parts.igem.org/Part:BBa_K1882016'>BBa_K1882016</a>)</p>
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                Here is a small demonstration for a block text.  Lorem ipsum
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</div>
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</div>
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<div class="contentRow">
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                sed diam voluptua. At vero eos et accusam et justo duo dolores
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        <div class="contentCell contentNarrowCell">
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                et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus
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             <p> Apa-Bam-Promotor-K230Rbind-RBS </p>
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            </p>
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            <table>
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                <caption>Uebersicht Saeuremienenarbeit:</caption>
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                <thead>
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                    <tr>
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                        <td>Year</td>
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                        <td>Slaves</td>
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                        <td>Income</td>
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                        <td>Bribes</td>
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                    </tr>
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                </thead>
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                <tbody>
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                    <tr>
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                        <td>2009</td>
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                        <td>9</td>
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                        <td>$1 mio.</td>
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                        <td>0</td>
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                    </tr>
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                    <tr>
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                        <td>2010</td>
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                        <td>90</td>
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                        <td>-$1 mio.</td>
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                        <td>$20,000</td>
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                    </tr>
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                    <tr>
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                        <td>2011</td>
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                        <td>100</td>
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                        <td>$10 mio.</td>
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                        <td>$50,000</td>
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                    </tr>
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                </tbody>
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            </table>
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             <p>
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                Don't forget to refer to tables in a text. Need
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                feature btw: Auto counters!
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            </p>
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         </div>
 
         </div>
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        <div class='contentCell contentSmallCell'>
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      <div class="contentCell contentMirijamsRaumZumAtmen"></div>
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            <p>
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<div class="contentCell">
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<p>composite part of BBa_J23110; BBa_B0032
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<p>(<a href='http://parts.igem.org/Part:BBa_K1882017'>BBa_K1882017</a>)</p>
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</div>
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</div>
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            </p>
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<div class="contentRow">
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            <table>
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        <div class="contentCell contentNarrowCell">
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                <caption>Uebersicht Saeuremienenarbeit:</caption>
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            <p> Apa-Bam-Promotor-C7C7bind-RBS </p>
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                <thead>
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                    <tr>
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                        <td>Year</td>
+
−
                        <td>Slaves</td>
+
−
                        <td>Income</td>
+
−
                        <td>Bribes</td>
+
−
                    </tr>
+
−
                </thead>
+
−
                <tbody>
+
−
                    <tr>
+
−
                        <td>2009</td>
+
−
                        <td>9</td>
+
−
                        <td>$1 mio.</td>
+
−
                        <td>0</td>
+
−
                    </tr>
+
−
                    <tr>
+
−
                        <td>2010</td>
+
−
                        <td>90</td>
+
−
                        <td>-$1 mio.</td>
+
−
                        <td>$20,000</td>
+
−
                    </tr>
+
−
                    <tr>
+
−
                        <td>2011</td>
+
−
                        <td>100</td>
+
−
                        <td>$10 mio.</td>
+
−
                        <td>$50,000</td>
+
−
                    </tr>
+
−
                </tbody>
+
−
            </table>
+
 
         </div>
 
         </div>
−
    </div>
+
      <div class="contentCell contentMirijamsRaumZumAtmen"></div>
 +
<div class="contentCell">
 +
<p>composite part of BBa_J23110, BBa_B0032
 +
<p>(<a href='http://parts.igem.org/Part:BBa_K1882018'>BBa_K1882018</a>)</p>
 +
</div>
 +
</div>  
 
</div>
 
</div>
 
</html>
 
</html>

Latest revision as of 03:03, 20 October 2016

Phosphofructokinase 1

This part contains a 6-phosphofructoskinase from Lactobacillus johnsonii ATCC33200 that metabolizes fructose-6-phosphate to fructose-1,6-bisphosphate. This part is RFC 10 compatible. The part's codon usage is optimized for expression in lactobacilli. The codon usage of this part was optimized to balance DNA synthesis and expression in L. Johnsonii. Therefore, longterm and shortterm repeats had to be modified and biobrick restriction sites were removed. The part was modified for RFC10 at the prefix and suffix of the part.

(BBa_K1882005)

LDH-Promotor Phosphofructokinase

This part encodes BBa_K1882002 and BBa_K1882000 resulting in a L. johnsonii ATCC33200 optimized phosphofructokinase under the control of a constitutive LDH-promotor. This leads to overexpression of the phosphofructokinase in L. johnsonii ATCC33200 when transferring this part into a lactobacillus expression vector.

(BBa_K1882001)

LDH-Promotor

This part contains a the promoter of the L. johnsonii ATCC33200 lactate dehydrogenase, a constitutively expressed protein. This part is RFC10 compatible

(BBa_K1882002)

Fructose Transporter

This part encodes a carbohydrate transporter from L. johnsonii ATCC 33200 that imports fructose into the target cell. In this process, fructose is phosphorylated by an N-phosphohistidine residue to yield fructose-6-phosphate and histidine. The protein is part of the ABC transporter superfamily. This part ist RFC 10 compatible. The part's codon usage is optimized for expression in lactobacilli. The part was optimized to balance DNA synthesis by IDT and expression in L. johnsonii. Therefore longterm and shortterm repeats had to modified and biobrick restriction sites were removed.The part was modified for RFC10 at the prefix and suffix of the part.

(BBa_K1882006)

LDH-Promotor

This part contains a the promoter of the L. johnsonii ATCC33200 lactate dehydrogenase, a constitutively expressed protein. This part is RFC10 compatible

(BBa_K1882007)

Ketohexokinase

This part encodes a ketohexokinase (ATP:D-fructose 1-phosphotransferase) from Rattus norvegicus optimized for expression in Saccharomyces cerevisiae. The ketohexokinase converts fructose to fructose-1-phosphate using ATP to perform the phosphorylation. This protein is not common in yeast and functions as toxin of the cell by trapping phosphate and therefore ATP in fructose-1-phosphate, not being able to be further metabolized. This result stop of growth of transformed yeast in fructose containing media (Donaldson et al., 1993). This part is RFC25 and RFC10 compatible.

(BBa_K1882000)

ApaI-constPromotor

This part encodes for BBa_J23102 with an ApaI restriction site upstream of it. Combination with BBa_K1882010 enables this part to clone a construct in a RFC compatible plasmid.

(BBa_K1882009)

Terminator-Bam-Hind

This part encodes for the rrnB T1 Terminator (BBa_B0010) followed by a T7 Terminator (BBa_B0012) and BamHI and HindIII restriction sites. Combination with BBa_K1882009 enables this part to clone a construct in an RFC compatible plasmid.

(BBa_K1882010)

RBS-PezT

This part encodes for a codon optimized version of UDP-N-acetylglucosamine kinase (PezT toxin). Upon expression the toxin produces UDP-N-acetyl-alpha-D-glucosamine 3'-phosphate which inhibits MurA and therefore cell wall synthesis in E. coli as well as L. johnsonii. Besides the sequence for the PezT toxin this part contains an upstream located ribosome binding site and could therefore be cloned direct behind any promoter of choice.

(BBa_K1882011)

ZF ZFN-L

This part contains the zinc finger ZF ZFN-L, see also Lombardo et al., 2007. The part is compatible with RFC10.

(BBa_K1882012)

ZF K230R

This part contains the zinc finger ZFK230R, see also Lee et al., 2010. The part is compatible with RFC10.

(BBa_K1882013)

ZF-C7C7

This part contains the zinc finger ZF-C7C7, see also Liu et al., 1997. The part is compatible with RFC10.

(BBa_K1882014)

Kill 1

This part is a composite part of the following components: Apa-pRha + RBS-PezT + Terminator-Bam-Hind. It is RFC10 compatible, and can be used for negative selection.

(BBa_K1882015)

Apa-Bam-Promotor-ZFNLbind-RBS

composite part of BBa_J23110; BBa_B0032

(BBa_K1882016)

Apa-Bam-Promotor-K230Rbind-RBS

composite part of BBa_J23110; BBa_B0032

(BBa_K1882017)

Apa-Bam-Promotor-C7C7bind-RBS

composite part of BBa_J23110, BBa_B0032

(BBa_K1882018)