Difference between revisions of "Team:Denver Biolabs/Parts"
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<td><a href="http://parts.igem.org/Part:BBa_K2111000">BBa_K2111000</a></td> | <td><a href="http://parts.igem.org/Part:BBa_K2111000">BBa_K2111000</a></td> | ||
<td>RI7_FLAG_OXTR: | <td>RI7_FLAG_OXTR: | ||
| − | This part is a chimeric receptor with a FLAG tag. It is composed of the ligand | + | This part is a chimeric receptor with a FLAG tag. It is composed of the ligand binding domain of the human oxytocin receptor, |
flanked by the N and C termini of the rat RI7 olfactory receptor. It was designed using the DIY receptor design guide created | flanked by the N and C termini of the rat RI7 olfactory receptor. It was designed using the DIY receptor design guide created | ||
by the 2012 TU Delft iGEM team (https://2012.igem.org/Team:TU-Delft/receptordesign). This part is intended to express in the | by the 2012 TU Delft iGEM team (https://2012.igem.org/Team:TU-Delft/receptordesign). This part is intended to express in the | ||
Latest revision as of 03:52, 20 October 2016
Team:Denver_Biolabs
Part Table
| Part Name | Description |
|---|---|
| BBa_K2111000 | RI7_FLAG_OXTR: This part is a chimeric receptor with a FLAG tag. It is composed of the ligand binding domain of the human oxytocin receptor, flanked by the N and C termini of the rat RI7 olfactory receptor. It was designed using the DIY receptor design guide created by the 2012 TU Delft iGEM team (https://2012.igem.org/Team:TU-Delft/receptordesign). This part is intended to express in the membrane of a yeast cell and - upon oxytocin ligand binding - activate the mating pathway and the inducible FUS1 promotor. This allows us to leverage a reporter protein under control of FUS1 to detect the presence of oxytocin. |
| BBa_K2111001 | PGK1_OXTR: This is a composite of the PGK1 promoter (BBa_K1652000) and RI7_FLAG_OXTR (BBa_K2111000). The PGK1 (pPGK1Gx) is a S. cerevisiae optimized constitutive promoter to facilitate the expression of RI7_FLAG_OXTR. |
| BBa_K2111002 | FUS1 Promoter: The FUS1 promoter is from the yeast mating pathway. It is induced with pheromones from the opposite mating type. |
| BBa_K2111003 | FUS1_AmilCP: This is a composite of the FUS1 promoter (BBa_K2111002) and amilCP blue chromoprotein (BBa_K592009). It is a reporter part used to visualize activation of the yeast mating pathway. |
| BBa_K2111004 | FUS1_RFP: This is a composite of the FUS1 promoter (BBa_K2111002) and RFP (BBa_E1010). It is a reporter part used to visualize activation of the yeast mating pathway. |
| BBa_K2111005 | FUS1_iLOV: This is a composite of the FUS1 promoter (BBa_K2111002) and iLOV (BBa_K660004). It is a reporter part used to visualize activation of the yeast mating pathway. |
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