Difference between revisions of "Team:Lethbridge HS/Results"

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{{Lethbridge_HS}}
+
{{Team:Lethbridge_HS/bootstrapcss}}  
<html>
+
<html lang="en">
 +
    <head>
 +
       
 +
       
 +
       
 +
        <!-- Wiki Style Reset (From U of C)-->
 +
    <style type="text/css">
 +
      /* Reset the wiki style by overriding with a reset stylesheet.
 +
        The relevant wiki css rules are reset by using rules with higher priority.
 +
        (Written by Florian Albrecht)
 +
        Shoutout to the 2016 U of C wiki */
 +
t
 +
      /* Start general reset ================================================== */
 +
        html body {font-size: inherit;font-family: inherit;background-color: transparent;color: inherit;padding: 0;}
 +
        div#globalWrapper {font-size: inherit;margin: 0;padding: 0;position: static;width: auto;}
 +
      /* Start top-section reset ============================================== */
 +
        div#top-section {border: 0;height: auto;margin: 0;position: static;width: auto;}
 +
        div#p-logo {height: auto;position: static;width: auto;z-index: auto;}
 +
        div#p-logo a, div#p-logo a:hover {text-decoration: none; font-family: 'Roboto', sans-serif;}
 +
        div#menubar {font-family: inherit;font-size: inherit;line-height: inherit;position: static;top: 0px;white-space: normal;width: auto;z-index: auto;}
 +
        div.left-menu, div.left-menu a {color: inherit;left: 0;text-align: left;text-transform: none;font-family: 'Roboto', sans-serif;}
 +
        div#menubar ul {color: inherit;list-style: disc outside none;margin: 0;padding: 0; font-family: 'Roboto', sans-serif;}
 +
        div#menubar li {cursor: auto;display: list-item;padding: 0;margin: 0;position: static; font-family: 'Roboto', sans-serif;}
 +
        div.left-menu li a {padding: 0; font-family: 'Roboto', sans-serif;}
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        div.left-menu:hover {color: inherit;background-color: transparent;}
 +
        div.left-menu:hover a {color: inherit;font-family: 'Roboto', sans-serif;}
 +
        div.right-menu, div.right-menu a {color: inherit;right: 0;text-align: left;font-family: 'Roboto', sans-serif;}
 +
        div.right-menu li a {background-color: transparent;color: inherit;padding: 0;font-family: 'Roboto', sans-serif;}
 +
        div.right-menu li a:hover {color: inherit;text-decoration: none;font-family: 'Roboto', sans-serif;}
 +
        div#search-controls {background-color: transparent;height: auto;position: static;right: 0;text-align: left;top: 0;width: auto;z-index: auto;}
 +
      /* Start content reset ================================================== */
 +
        div#content {background: transparent;border: 0;color: inherit;line-height: inherit;margin: 0;padding: 0;position: static;width: auto;z-index: auto;}
 +
        body li {margin: 0; font-family: 'Roboto', sans-serif;}
 +
        body h1, body h2, body h3, body h4, body h5, body h6 {background: transparent;border: 0;color: inherit;padding: 0; font-family: 'Roboto', sans-serif;}
 +
        body .firstHeading {margin: 0;}
 +
        div#contentSub {color: inherit;font-size: inherit;line-height: inherit;margin: 0;width: auto;}
 +
        div#catlinks {background-color: transparent;border: 0;clear: both;margin: 0;padding: 0;}
 +
      /* Start footer reset =================================================== */
 +
        div#footer-box {background-color: transparent;border: 0;margin: 0;padding: 0;width: auto;}
 +
        div#footer {color: inherit;font-size: inherit;text-align: left;}
 +
        div#f-poweredbyico, div#f-copyrightico {float: none;}
 +
        div#footer li {margin: 0;display: list-item; font-family: 'Roboto', sans-serif;}
 +
      </style>
  
 +
      <!-- SideMenu for iGEM Stuff -->
 +
      <style type="text/css">
 +
      #sideMenu{width: 200px;position: absolute;top: 20px;left: 1020px;z-index: 10;padding-top: 0px;padding-bottom: 15px;padding-left: 15px;padding-right: 15px;background-color: white;text-align: left;display: none;z-index: 9996;}
 +
      #content{width: 100%;padding: 0px;margin-left: 0px;}
 +
      #top_title{overflow: hidden;display: none;}
 +
      #top_menu_14{height: 20px;}
 +
      #top_menu_under{height: 0px;}
 +
      #globalWrapper{padding-bottom: 200px;}
 +
      </style>
  
<div class="column full_size" >
+
      <!-- Custom Styling -->
 +
      <style>
 +
      a {list-style-image: none;}
 +
      ul {list-style-image: none;}
 +
      ol {list-style-image: none;}
 +
      li {list-style-image: none;}
 +
      </style>
  
<p>Here you can describe the results of your project and your future plans. </p>
 
  
<h5>What should this page contain?</h5>
 
<ul>
 
<li> Clearly and objectively describe the results of your work.</li>
 
<li> Future plans for the project </li>
 
<li> Considerations for replicating the experiments </li>
 
</ul>
 
  
</div>
 
  
<div class="column half_size" >
+
<style>
 +
body{
 +
  background-color: white; 
 +
    font-family: 'Open Sans', sans-serif;
 +
}
 +
.jumbotron {
 +
    background-color: white;
 +
    position: relative;
 +
}
 +
.slide{
 +
    width:100%;
 +
   
 +
    margin: 0;
 +
   
 +
}
 +
#slide0{
 +
    background-image: url("https://static.igem.org/mediawiki/2016/5/54/T--Lethbridge_HS--labresultspic--.jpeg");
 +
    background-size:cover;
 +
    background-position: center center;
 +
    background-repeat: no-repeat;
 +
    height: 50vh;
 +
    overflow: hidden;
 +
    margin: 0;
 +
    padding-top: 5%;
 +
    background-color: #333333;
 +
}
 +
#firsttitle{
 +
    vertical-align: center;
 +
    margin-left: auto;
 +
    margin-right: auto;
 +
    text-align: left;
 +
    color: white;
 +
    vertical-align: center;
 +
position:absolute;
 +
bottom:0;
  
 +
 
 +
font-size: 8em;
 +
}
 +
#secondtitle{
 +
    vertical-align: center;
 +
    margin-left: auto;
 +
    margin-right: auto;
 +
    text-align: center;
 +
    color: white;
 +
    vertical-align: middle;
 +
  top: 25%;
 +
font-size: 2em;
 +
}
 +
.slidesecondlist{
 +
    list-style-type: none;
 +
    margin-left: 15%;
 +
}
 +
.slidesecondlist ul{
 +
    margin: 0;
 +
    padding: 20px;
 +
    background-color: none;
 +
}
 +
#slidesecond{
  
<h5> Project Achievements </h5>
+
    overflow: hidden;
 +
    margin: 0;
 +
    padding-top: 0;
 +
    padding: 0;
 +
    background-color: #eee7dd;
 +
}
  
<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
+
#slide_project_0{
 +
    background-image: url("images/funnyteamcover.png");
 +
    background-size:cover;
 +
    background-position: center -10%;
 +
    background-repeat: no-repeat;
 +
    height: 90%;
 +
    overflow: hidden;
 +
}
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#slide_team_0{
 +
    background-image: url("images/IMG_4553.jpg");
 +
    background-size:cover;
 +
    background-position: center -10%;
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    background-repeat: no-repeat;
 +
    height: 90%;
 +
    overflow: hidden;
 +
}
 +
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 +
    background-image: url("images/funnyteamcover.png");
 +
    background-size:cover;
 +
    background-position: center -10%;
 +
    background-repeat: no-repeat;
 +
    height: 90%;
 +
    overflow: hidden;
 +
}
 +
#slide0title{
 +
    color: white;
 +
    display: block;
 +
    margin: auto;
 +
    text-align: center;
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}
 +
#blooddrop{
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    position: absolute;
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 +
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    z-index: 1;
 +
    -webkit-animation: fadeInOut 2s infinite;
 +
    -moz-animation: fadeInOut 2s infinite;
 +
    -o-animation: fadeInOut 2s infinite;
 +
    animation: fadeInOut 2s infinite;
 +
}
 +
#slide1{
 +
    /*background-color:#eee7dd;*/
 +
    background-color:white;
 +
  /* background-image: url("images/289680_1231091663_large.jpg");
 +
    background
 +
    background-size:cover;
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    background-position: center center;
 +
    background-repeat: no-repeat;*/
 +
    color: black;
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}
 +
#slide1Sub{
 +
    margin-left: 10%;
 +
    margin-right: 10%;
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}
 +
#slide2{
 +
  /* background-image: url("images/anotherteamcover.png");*/
 +
    background-color: green;
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    background-size: cover;
 +
    background-position: center center;
 +
    background-repeat: no-repeat;
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 +
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 +
   
 +
}
 +
#slide3{
 +
    background-color: #cf4848;
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    height: 90%;
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    padding-top:2%;
 +
}ding-top:1%;
 +
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 +
    color:red;
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    font-size:60px;
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    margin-top:0%;
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    color:red;
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    text-align: center;
 +
    font-size:50px;
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 +
#slide1para{
 +
    font-size: 1.1em;
 +
    color: black;
 +
}
 +
.logo{
 +
    float:left;
 +
    margin-left:9%;
 +
    margin-top: 1%;
 +
}
 +
.navbar-header{
 +
    margin-top:-20px;
 +
}
 +
.navbar-default{
 +
  /** box-shadow: 0px 8px 6px -6px #999;**/
 +
 +
}
 +
.navbar-default .navbar-nav>li>a {
 +
    color: white;
 +
    font-size: 15px;
 +
    padding-top:2px;
 +
    padding-top:2px;
 +
    margin-top: 12px;
 +
}
 +
.navbar-collapse.collapse {
 +
    padding-bottom: 6px;
 +
    padding-top: 3px;
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}
 +
.navbar-default .navbar-nav>.active>a, .navbar-default .navbar-nav>.active>a:focus, .navbar-default .navbar-nav>.active>a:hover {
 +
    color: white;
 +
    background-color: transparent;
 +
}
  
<ul>
+
.removeNavBarColor{
<li>A list of linked bullet points of the successful results during your project</li>
+
    background-color: transparent;
<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
+
   
</ul>
+
}
 +
.makeNavBarColor{
 +
  /** background-color: #CC141C ;*/
 +
    background-color: #dc4640 ;
 +
   
 +
   
 +
}
  
</div>
+
.navbar-default .navbar-nav>li>a{
 +
    padding-top: 30px;
 +
    margin-top: 0;
 +
    margin-bottom: 0%;
 +
    padding-bottom: 15px;
 +
    color:white;
 +
}
 +
.navbar-collapse.collapse{
 +
    padding-top: 0;
 +
    padding-bottom: 0;
 +
}
 +
@-webkit-keyframes fadeInOut{
 +
    0% { opacity: 0;}
 +
    50% { opacity: 1; }
 +
    100% { opacity:0;}
 +
}
 +
@-moz-keyframes fadeInOut{
 +
    0% { opacity: 0;}
 +
    50% { opacity: 1; }
 +
    100% { opacity:0;}
 +
}
 +
@-o-keyframes fadeInOut{
 +
    0% { opacity: 0;}
 +
    50% { opacity: 1; }
 +
    100% { opacity:0;}
 +
}
 +
@keyframes fadeInOut{
 +
    0% { opacity: 0;}
 +
    50% { opacity: 1; }
 +
    100% { opacity:0;}
 +
}
  
 +
[class^="flaticon-"]:before, [class*=" flaticon-"]:before, [class^="flaticon-"]:after, [class*=" flaticon-"]:after {
 +
        font-family: Flaticon;
 +
        font-size: 30px;
 +
        font-style: normal;
 +
        margin-left: 0px;
 +
        color: white;
 +
   
 +
    }
  
<div class="column half_size" >
 
  
<h5>Inspiration</h5>
+
.glyph {
<p>See how other teams presented their results.</p>
+
        display: inline-block;
<ul>
+
        width: 9em;
<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
+
        margin: 1em;
<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
+
        text-align: center;
<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
+
        vertical-align: top;
</ul>
+
        background: #FFF;
 +
    float: left;
 +
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 +
    .glyph .glyph-icon {
 +
        display:inline-block;
 +
        float: left;
 +
       
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        font-family:"Flaticon";
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        font-size: 64px;
 +
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 +
.glyph .glyph-icon:before {
 +
        font-size: 64px;
 +
        color: white;
 +
        margin-left: 0;
 +
    }
  
</div>
+
.menuIcon{
 +
 +
}
 +
.active{
 +
    background-color: red;
 +
    color: white !important;
 +
}
 +
[class^="active"]:before, [class*="active"]:before, [class^="active"]:after, [class*="active"]:after {
 +
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 +
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        font-size: 20px;
  
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.menuIcon2:after { 
  
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        font-size: 40px;
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 +
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.sidebar{
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.profilePicBox{
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 +
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 +
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text-align:center;
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margin-left:auto;
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margin-right:auto;
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 +
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 +
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 +
/*****************************************Time line*********************************/
 +
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 +
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 +
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 +
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 +
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 +
/**************************How to make font size on notebook responsive*******/
 +
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 +
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                                    <a href="https://2016.igem.org/Team:Lethbridge_HS/Description">DESCRIPTION</a>
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                                    <a href="https://2016.igem.org/Team:Lethbridge_HS/Design">DESIGN</a>
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<a href="https://2016.igem.org/Team:Lethbridge_HS/Prototype">PROTOTYPE</a>
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<a href="https://2016.igem.org/Team:Lethbridge_HS/Proof">PROOF OF CONCEPT</a>
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                                    <a href="https://2016.igem.org/Team:Lethbridge_HS/Results">RESULTS</a>
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<a href="https://2016.igem.org/Team:Lethbridge_HS/Achievements">ACHIEVEMENTS</a>
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<a href="https://2016.igem.org/Team:Lethbridge_HS/HP/Silver">SILVER</a>
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  <a href="https://2016.igem.org/Team:Lethbridge_HS/HP/Gold">GOLD</a>
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                                    <a href="https://2016.igem.org/Team:Lethbridge_HS/Integrated_Practices">INTEGRATED HUMAN PRACTICES</a>
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<a href="https://2016.igem.org/Team:Lethbridge_HS/Engagement">ENGAGEMENT</a>
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                                    <a href="https://2016.igem.org/Team:Lethbridge_HS/Notebook">NOTEBOOK</a>
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<a href="https://2016.igem.org/Team:Lethbridge_HS/Methods">METHODS</a>
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                                    <a href="https://2016.igem.org/Team:Lethbridge_HS/Attributions">ATTRIBUTIONS</a>
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                                    <a href="https://2016.igem.org/Team:Lethbridge_HS/Collaborations">COLLABORATIONS</a>
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<div class="container-fluid" style="margin-left:10%;">
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            <h1 id="firsttitle" style=""> <b>RESULTS</b></h1>
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            <!--<div style="position:relative;">
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<img src="https://static.igem.org/mediawiki/2016/a/a4/T%E2%80%94lethbridgehs--graph_and.jpeg" width="100%">
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        <table class="table table-striped table-bordered">
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                                            <caption>Clot Times</caption>
 +
                                            <thead>
 +
                                                <tr>
 +
                                                    <th>Treatment</th>
 +
                                                    <th>Time</th>
 +
<th>Half Clot Time</th>
 +
                                                </tr>
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                                            </thead>
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                                            <tbody>
 +
                                                <tr>
 +
                                                    <td>70 mg heparin blood, 1 mL viper construct</td>
 +
                                                    <td>0</td>
 +
<td>31</td>
 +
                                                </tr>
 +
                                                <tr>
 +
                                                    <td>
 +
70 mg heparin blood, 1 mL PNRa
 +
</td>
 +
                                                    <td>1</td>
 +
<td>33</td>
 +
                                                </tr>
 +
                                                <tr>
 +
                                                    <td>
 +
70 mg heparin blood, 2 mL PNRA
 +
</td>
 +
                                                    <td>2</td>
 +
  <td>41</td>
 +
                                                </tr>
 +
                                                <tr>
 +
                                                    <td>
 +
70 mg heparin blood, 2 mL viper construct
 +
</td>
 +
                                                    <td>3</td>
 +
<td>54</td>
 +
                                                </tr>
 +
                                                <tr>
 +
                                                    <td>
 +
70 mg heparin blood, 0 cells
 +
</td>
 +
                                                    <td>4</td>
 +
<td>44</td>
 +
                                                </tr>
 +
                                                <tr>
 +
                                                    <td>
 +
71 mg heparin blood, 2 mL PNRA
 +
</td>
 +
                                                    <td>5</td>
 +
<td>57</td>
 +
                                                </tr>
 +
 +
<tr>
 +
                                                    <td>
 +
 +
71 mg heparin blood, 1 mL viper construct
 +
 +
</td>
 +
                                                    <td>6</td>
 +
<td>67</td>
 +
                                                </tr>
 +
 +
<tr>
 +
                                                    <td>
 +
 +
71 mg heparin blood, no cells
 +
 +
</td>
 +
                                                    <td>7</td>
 +
<td>48</td>
 +
                                                </tr>
 +
 +
                                            </tbody>
 +
                                        </table>
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<img src="https://static.igem.org/mediawiki/2016/5/55/T%E2%80%94lethbridgehs--bloodpics_and.jpeg" width="60%">
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<p><br>The half clot time of different treatments on the blood was tested and calculated. Specifically, the clotting time of our blood was tested with 70mg of heparin as well as 71mg of heparin in different trials, in addition to either 1mL or 2mL of PNRA or 1mL or 2mL of our construct in order to determine the effects of our construct on the blood clotting pathway. A positive control was also set by having blood with heparin with no cells, to ensure the blood did not spontaneously clot on its own. In general, we were able to show that our construct caused the blood to clot. Although these are preliminary tests, we plan to optimize our construct further in the future in order to determine the resulting flow rates after our construct is applied to ensure that it does not impact the rate of blood flow throughout the rest of the patient’s body.</br>
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Latest revision as of 03:55, 20 October 2016

Lethbridge HS iGEM 2016

RESULTS

Clot Times
Treatment Time Half Clot Time
70 mg heparin blood, 1 mL viper construct 0 31
70 mg heparin blood, 1 mL PNRa 1 33
70 mg heparin blood, 2 mL PNRA 2 41
70 mg heparin blood, 2 mL viper construct 3 54
70 mg heparin blood, 0 cells 4 44
71 mg heparin blood, 2 mL PNRA 5 57
71 mg heparin blood, 1 mL viper construct 6 67
71 mg heparin blood, no cells 7 48


The half clot time of different treatments on the blood was tested and calculated. Specifically, the clotting time of our blood was tested with 70mg of heparin as well as 71mg of heparin in different trials, in addition to either 1mL or 2mL of PNRA or 1mL or 2mL of our construct in order to determine the effects of our construct on the blood clotting pathway. A positive control was also set by having blood with heparin with no cells, to ensure the blood did not spontaneously clot on its own. In general, we were able to show that our construct caused the blood to clot. Although these are preliminary tests, we plan to optimize our construct further in the future in order to determine the resulting flow rates after our construct is applied to ensure that it does not impact the rate of blood flow throughout the rest of the patient’s body.