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− | <div class="topic"><p class="text_color">Safety of | + | <div class="topic"><p class="text_color">Safety of Pantide</p></div> |
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<p class="title">Safety<p> | <p class="title">Safety<p> | ||
− | <p class="content-2">The safety of our project consists of two aspects. One is the laboratory safety; the other is the safety of spraying | + | <p class="content-2">The safety of our project consists of two aspects. One is the laboratory safety; the other is the safety of spraying Pantide into the environment.</p> |
</div> | </div> | ||
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− | <p class="title">Safety of | + | <p class="title">Safety of Pantide</p> |
<p class="content-1">Processing Procedure in the Lab</p> | <p class="content-1">Processing Procedure in the Lab</p> | ||
− | <p class="content-2">In the future, we plan to spray | + | <p class="content-2">In the future, we plan to spray Pantide onto the environment. Therefore, we should guarantee that Pantide is safe to humans and harmless to the environment. As a result, we design a processing standard in the laboratory. </p> |
<ul style="list-style-image:none;list-style-type:decimal;" > | <ul style="list-style-image:none;list-style-type:decimal;" > | ||
<li class="list">Free of <i>E. coli</i> | <li class="list">Free of <i>E. coli</i> | ||
− | <br> The product we spray into the environment should not contain any genetically-modified organisms. So, after we expressed | + | <br> The product we spray into the environment should not contain any genetically-modified organisms. So, after we expressed Pantide with <i>E. coli</i>, the LB broth containing <i>E.coli</i> should be first sonicated by the sonicator to let Pantide be soluble in the LB broth. In this step, most of the living <i>E.coli</i> will be eliminated. In order to guarantee the sonicated LB broth lysate is free of <i>E. coli</i>, we boil the lysate for 1 hour. However, boiling the solution may induce Pantide degradation. We want to know the degradation time of boiling the lysate. Pantide are retained and we run SDS-PAGE to evaluate the amount of Pantide residue. (Figure. 1) |
+ | <div> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/6/66/NCTU_SAFETY1.jpeg" class="picture"> | ||
+ | <p class="content-image">Figure1. SDS-PAGE gel and the concentrations of boiling test to native Hv1a-lectin (17.1 kDa). The marked band shows that there is a significant decrease in Hv1a-lectin after 2 hours.</p> | ||
+ | </div> | ||
</li> | </li> | ||
− | <li class="list">Degradation of | + | <li class="list">Degradation of Pantide |
− | <br> To ensure that | + | <br> To ensure that Pantide will degrade into amino acids when it is released into the environment, we have done an experiment to prove that Pantide does degrade in the ultraviolet light. (See the <a href="https://2016.igem.org/Team:NCTU_Formosa/Model#title3" style="color:#44E287;">experiment</a>) Once we have known that Pantide will degrade in the ultraviolet light, we may conclude that Pantide will degrade in the natural light over time. With the degradation modeling simulation (See the <a href="https://2016.igem.org/Team:NCTU_Formosa/Model#title3" style="color:#44E287;">Modelling</a>), we may also apply the degradation rate into the automatic spraying device. |
</li> | </li> | ||
</ul> | </ul> | ||
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<div> | <div> | ||
− | <p class="content-2">For humans, | + | <p class="content-2">For humans, Pantide will not have any negative effect on human health. When the customer gets the agricultural produce from the market, most of the Pantide is thought to have degraded into amino acids and even there is some residue of Pantide on the surface of the product. Pantide is also harmless to humans. The three toxins we choose have been proved to be ineffective to mammals due to the structural difference between the voltage-gated ion channels between mammals and insects. It has been validated through references doing experiments on mice.</p> |
− | <p class="content-2">For the environment, | + | <p class="content-2">For the environment, Pantide is made of amino acids. So it is harmless for the environment. Instead, it can be as the fertilizer. When it is washed into the soil or in water resources, Pantide is ineffective to the microbiomes and the vertebrate in the ecosystem. Furthermore, Pantide is species-specific, so it will not harm the beneficial pollinator, Bees.</p> |
</div> | </div> |
Latest revision as of 03:08, 4 November 2016