Difference between revisions of "Team:NCTU Formosa/Basic Part"

 
(31 intermediate revisions by 4 users not shown)
Line 7: Line 7:
  
 
<link href="https://2016.igem.org/Team:NCTU_Formosa/wiki_reset?action=raw&ctype=text/css" rel="stylesheet">
 
<link href="https://2016.igem.org/Team:NCTU_Formosa/wiki_reset?action=raw&ctype=text/css" rel="stylesheet">
−
<link href="https://fonts.googleapis.com/css?family=Open+Sans:300" rel="stylesheet">
+
<link href="https://fonts.googleapis.com/css?family=Open+Sans:300,400" rel="stylesheet">
  
  
Line 110: Line 110:
 
.line .red {
 
.line .red {
 
   width:1px;    /*調line長度*/
 
   width:1px;    /*調line長度*/
−
   height:47%; /*line寬度*/
+
   height:0%; /*line寬度*/
 
   position:relative;
 
   position:relative;
  
Line 117: Line 117:
 
.line .blue {
 
.line .blue {
 
   width: 1px;  /*line寬度*/
 
   width: 1px;  /*line寬度*/
−
   height: 20%;  /*調line長度*/
+
   height: 0%;  /*調line長度*/
 
   position:relative;
 
   position:relative;
 
   left:51px;
 
   left:51px;
Line 252: Line 252:
 
     margin-top:40px;
 
     margin-top:40px;
 
     margin-bottom:30px;
 
     margin-bottom:30px;
 +
    font-weight:400;
 
}
 
}
  
Line 257: Line 258:
 
     font-size:15pt !important;
 
     font-size:15pt !important;
 
     text-align:justify !important;
 
     text-align:justify !important;
−
    text-indent:2em;
 
 
     color:#F3F7F7;
 
     color:#F3F7F7;
−
     padding-top:15px;
+
     padding-top:10px;
−
     padding-bottom:15px;
+
     padding-bottom:10px;
 
}
 
}
 
/*second title*/
 
/*second title*/
Line 329: Line 329:
 
.picture{
 
.picture{
 
     position:relative;
 
     position:relative;
−
    left:7vw;
+
     width:20%;
−
     width:80%;
+
 
}
 
}
  
Line 361: Line 360:
 
    
 
    
 
           <div class="node">
 
           <div class="node">
−
           <div class="topic"><p class="text_color">Composite part</p></div>
+
           <div class="topic"><p class="text_color">Basic Parts</p></div>
−
            <div class="active-circle">
+
−
              <div class="splash"></div>
+
−
              <div class="cover">   
+
−
              </div>
+
−
            </div>
+
−
            <div class="inactive-circle">
+
−
              <div class="small-cover">
+
−
              </div>
+
−
            </div>
+
−
          </div>
+
−
 
+
−
          <div class="node">
+
−
          <div class="topic"><p class="text_color">Pantide without lectin</p></div>
+
−
            <div class="active-circle">
+
−
              <div class="splash"></div>
+
−
              <div class="cover">   
+
−
              </div>
+
−
            </div>
+
−
            <div class="inactive-circle">
+
−
              <div class="small-cover">
+
−
              </div>
+
−
            </div>
+
−
          </div>
+
−
 
+
−
         
+
−
          <div class="node">
+
−
          <div class="topic"><p class="text_color">Pantide with lectin</p></div>
+
 
             <div class="active-circle">
 
             <div class="active-circle">
 
               <div class="splash"></div>
 
               <div class="splash"></div>
Line 400: Line 372:
 
           </div>
 
           </div>
  
−
 
−
          <div class="node">
 
−
          <div class="topic"><p class="text_color">Pantide with Gs linker</p></div>
 
−
            <div class="active-circle">
 
−
              <div class="splash"></div>
 
−
              <div class="cover">   
 
−
              </div>
 
−
            </div>
 
−
            <div class="inactive-circle">
 
−
              <div class="small-cover">
 
−
              </div>
 
−
            </div>
 
−
          </div>
 
−
         
 
−
 
 
−
 
 
     </nav>
 
     </nav>
  
 
<!--圖片-->
 
<!--圖片-->
 
   <div class="img-container">
 
   <div class="img-container">
−
         <img src="https://static.igem.org/mediawiki/2016/b/be/NCTU_composite_parts.png" class="main-img" width="100%">
+
         <img src="https://static.igem.org/mediawiki/2016/f/fc/NCTU_BASIC_PARTS_BIG_PICTURE.png" class="main-img" width="100%">
 
   </div>
 
   </div>
 
   <div>
 
   <div>
Line 431: Line 387:
  
 
<!--------------------wiki content----------------------->
 
<!--------------------wiki content----------------------->
 +
 +
 
<section class="content_container">
 
<section class="content_container">
 
<div>
 
<div>
−
<p class="title">Basic part</p>
+
<p class="title">Basic Parts</p>
−
<p class="content">We have known the function of the three toxic peptides of Pantide, ω-hexatoxin-Hv1a, µ-segestritoxin-Sf1a, and Orally Active Insecticidal Peptide (OAIP) respectively and they are easy to express with the help of the bacteria <i>E. coli BL21 Rosetta Gami strain</i>. So, we then start to create BioBricks of these three toxins which we have obtained the nucleotide sequences. We provide the four basic parts with the single genes of Hv1a, Sf1a, OAIP, and snowdrop lectin. </p>
+
<p class="content">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;We have known the function of the three toxic peptides of PANTIDE, Omega-hexatoxin-Hv1a, µ-segestritoxin-Sf1a, and Orally Active Insecticidal Peptide (OAIP) respectively and they are easy to express with the help of the bacteria <i>E. coli</i> BL21 Rosetta-Gami strain. So, we then start to create BioBricks of these three toxins which we have obtained the nucleotide sequences. We provide the four basic parts with the single genes of Hv1a, Sf1a, OAIP, and snowdrop lectin. </p>
−
 
+
  
  
 
<!----------basic-hv1a-------->
 
<!----------basic-hv1a-------->
−
<a href="#" class="biobrick"><p class="content-1">BBa_K1974001</p></a>
+
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1974001" class="biobrick"><p class="content-1">BBa_K1974001</p></a>
  
 
<div>
 
<div>
−
       <img src="#" class="picture">
+
       <img src="https://static.igem.org/mediawiki/2016/5/57/2016_NCTU-FORMOSA_Hv1a.png" class="picture">
 
</div>
 
</div>
  
Line 448: Line 405:
 
Hv1a
 
Hv1a
 
</p>
 
</p>
−
<p class="content">Pantide that contains the gene coding for Hv1a. </p>
+
<p class="content">PANTIDE that contains the gene coding for Hv1a. </p>
  
  
  
 
<!--------basic-sf1a----------->
 
<!--------basic-sf1a----------->
−
<a href="#" class="biobrick"><p class="content-1">BBa_........</p></a>
+
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1974002" class="biobrick"><p class="content-1">BBa_K1974002</p></a>
  
 
<div>
 
<div>
−
       <img src="#" class="picture">
+
       <img src="https://static.igem.org/mediawiki/2016/b/be/2016_NCTU-FORMOSA_Sf1a.png" class="picture">
 
</div>
 
</div>
  
Line 462: Line 419:
 
Sf1a
 
Sf1a
 
</p>
 
</p>
−
<p class="content">Pantide that contains the gene coding for Sf1a.</p>
+
<p class="content">PANTIDE that contains the gene coding for Sf1a.</p>
  
  
  
 
<!-------basic-OAIP------------->
 
<!-------basic-OAIP------------->
−
<a href="#" class="biobrick"><p class="content-1">BBa_........</p></a>
+
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1974003" class="biobrick"><p class="content-1">BBa_K1974003</p></a>
  
 
<div>
 
<div>
−
       <img src="#" class="picture">
+
       <img src="https://static.igem.org/mediawiki/2016/f/fb/2016_NCTU-FORMOSA_OAIP.png" class="picture">
 
</div>
 
</div>
  
 
<p class="content">OAIP</p>
 
<p class="content">OAIP</p>
−
<p class="content">Pantide that contains the gene coding for OAIP. </p>
+
<p class="content">PANTIDE that contains the gene coding for OAIP. </p>
  
  
Line 482: Line 439:
 
<!---------------Snowdrop lectin---------------->
 
<!---------------Snowdrop lectin---------------->
  
−
<a href="#" class="biobrick"><p class="content-1">BBa_........</p></a>
+
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1974020" class="biobrick"><p class="content-1">BBa_K1974020</p></a>
  
 
<div>
 
<div>
−
       <img src="#" class="picture">
+
       <img src="https://static.igem.org/mediawiki/2016/7/72/2016_NCTU-FORMOSA_L.png" class="picture">
 
</div>
 
</div>
  
 
<p class="content">
 
<p class="content">
−
Snowdrop lectin
+
Snowdrop Lectin
 
</p>
 
</p>
−
<p class="content">Pantide that contains the gene coding for snowdrop lectin.</p>
+
<p class="content">PANTIDE that contains the gene coding for snowdrop lectin.</p>
  
  
  
 
<!---------Gs linker---------->
 
<!---------Gs linker---------->
−
<a href="#" class="biobrick"><p class="content-1">BBa_........</p></a>
+
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1974030" class="biobrick"><p class="content-1">BBa_K1974030</p></a>
  
 
<div>
 
<div>
−
       <img src="#" class="picture">
+
       <img src="https://static.igem.org/mediawiki/2016/0/0b/2016_NCTU-FORMOSA_GS.png" class="picture">
 
</div>
 
</div>
  
−
<p class="content">
+
<p class="content">GS linker</p>
−
Gs linker
+
−
</p>
+
  
  
 
<p class="content">
 
<p class="content">
−
Pantide that contains the gene coding for Gs linker.
+
PANTIDE that contains the gene coding for GS linker.
  
 
</p>
 
</p>
−
 
  
  
Line 517: Line 471:
 
</div>
 
</div>
 
</section>
 
</section>
 +
 +
  
  

Latest revision as of 03:12, 4 November 2016

Basic Parts

      We have known the function of the three toxic peptides of PANTIDE, Omega-hexatoxin-Hv1a, µ-segestritoxin-Sf1a, and Orally Active Insecticidal Peptide (OAIP) respectively and they are easy to express with the help of the bacteria E. coli BL21 Rosetta-Gami strain. So, we then start to create BioBricks of these three toxins which we have obtained the nucleotide sequences. We provide the four basic parts with the single genes of Hv1a, Sf1a, OAIP, and snowdrop lectin.

BBa_K1974001

Hv1a

PANTIDE that contains the gene coding for Hv1a.

BBa_K1974002

Sf1a

PANTIDE that contains the gene coding for Sf1a.

BBa_K1974003

OAIP

PANTIDE that contains the gene coding for OAIP.

BBa_K1974020

Snowdrop Lectin

PANTIDE that contains the gene coding for snowdrop lectin.

BBa_K1974030

GS linker

PANTIDE that contains the gene coding for GS linker.