Difference between revisions of "Team:NCTU Formosa/Composite Part"

 
(40 intermediate revisions by 5 users not shown)
Line 7: Line 7:
  
 
<link href="https://2016.igem.org/Team:NCTU_Formosa/wiki_reset?action=raw&ctype=text/css" rel="stylesheet">
 
<link href="https://2016.igem.org/Team:NCTU_Formosa/wiki_reset?action=raw&ctype=text/css" rel="stylesheet">
−
<link href="https://fonts.googleapis.com/css?family=Open+Sans:300" rel="stylesheet">
+
<link href="https://fonts.googleapis.com/css?family=Open+Sans:300,400" rel="stylesheet">
  
  
Line 110: Line 110:
 
.line .red {
 
.line .red {
 
   width:1px;    /*調line長度*/
 
   width:1px;    /*調line長度*/
−
   height:80%; /*line寬度*/
+
   height:47%; /*line寬度*/
 
   position:relative;
 
   position:relative;
  
Line 117: Line 117:
 
.line .blue {
 
.line .blue {
 
   width: 1px;  /*line寬度*/
 
   width: 1px;  /*line寬度*/
−
   height: 7%;  /*調line長度*/
+
   height: 20%;  /*調line長度*/
 
   position:relative;
 
   position:relative;
 
   left:51px;
 
   left:51px;
Line 243: Line 243:
 
     width:60vw;
 
     width:60vw;
 
     height:auto;
 
     height:auto;
 +
 
}
 
}
 
.title{
 
.title{
Line 250: Line 251:
 
     color:rgb(0, 206, 255);
 
     color:rgb(0, 206, 255);
 
     text-shadow:1px 1px 10px rgb(0, 206, 255);
 
     text-shadow:1px 1px 10px rgb(0, 206, 255);
−
     margin-top:20px;
+
     margin-top:40px;
 +
    margin-bottom:30px;
 +
    font-weight:400;
 
}
 
}
  
Line 263: Line 266:
 
/*second title*/
 
/*second title*/
 
.content-1{
 
.content-1{
−
     font-size:19pt !important;
+
     font-size:20pt !important;
 
     text-align:justify;
 
     text-align:justify;
 
     color:#44E287;
 
     color:#44E287;
Line 270: Line 273:
 
     padding-bottom:10px
 
     padding-bottom:10px
 
}
 
}
−
.content-2{
+
 
−
    font-size:17pt !important;
+
−
    text-align:justify;
+
−
    color:#F3F7F7;
+
−
    font-weight: bold !important;
+
−
    padding-top:30px;
+
−
    padding-bottom:10px
+
−
}
+
 
.content-image{
 
.content-image{
 
     text-align:center !important;
 
     text-align:center !important;
Line 335: Line 331:
 
.picture{
 
.picture{
 
     position:relative;
 
     position:relative;
−
    left:7vw;
+
 
−
     width:80%;
+
     width:70%;
 
}
 
}
  
Line 367: Line 363:
 
    
 
    
 
           <div class="node">
 
           <div class="node">
−
           <div class="topic"><p class="text_color">Composite part</p></div>
+
           <div class="topic"><p class="text_color">Composite Parts</p></div>
 
             <div class="active-circle">
 
             <div class="active-circle">
 
               <div class="splash"></div>
 
               <div class="splash"></div>
Line 380: Line 376:
 
    
 
    
 
           <div class="node">
 
           <div class="node">
−
           <div class="topic"><p class="text_color">Pantide without lectin</p></div>
+
           <div class="topic"><p class="text_color">PANTIDE without Lectin</p></div>
 
             <div class="active-circle">
 
             <div class="active-circle">
 
               <div class="splash"></div>
 
               <div class="splash"></div>
Line 394: Line 390:
 
            
 
            
 
           <div class="node">
 
           <div class="node">
−
           <div class="topic"><p class="text_color">Pantide with lectin</p></div>
+
           <div class="topic"><p class="text_color">PANTIDE with Lectin</p></div>
 
             <div class="active-circle">
 
             <div class="active-circle">
 
               <div class="splash"></div>
 
               <div class="splash"></div>
Line 408: Line 404:
  
 
           <div class="node">
 
           <div class="node">
−
           <div class="topic"><p class="text_color">Pantide with Gs linker</p></div>
+
           <div class="topic"><p class="text_color">PANTIDE with GS Linker</p></div>
 
             <div class="active-circle">
 
             <div class="active-circle">
 
               <div class="splash"></div>
 
               <div class="splash"></div>
Line 426: Line 422:
 
<!--圖片-->
 
<!--圖片-->
 
   <div class="img-container">
 
   <div class="img-container">
−
         <img src="https://static.igem.org/mediawiki/2016/b/be/NCTU_composite_parts.png" class="main-img" width="100%">
+
         <img src="https://static.igem.org/mediawiki/2016/9/98/NCTU_COMPOSITE_BIG_PICTURE.png
 +
" class="main-img" width="100%">
 
   </div>
 
   </div>
 
   <div>
 
   <div>
Line 439: Line 436:
 
<section class="content_container">
 
<section class="content_container">
 
<div>
 
<div>
−
<p class="title">Composite part</p>
+
<p class="title">Composite Parts</p>
−
<p class="content">We have known the function of the three toxic peptides of Pantide, ω-hexatoxin-Hv1a, µ-segestritoxin-Sf1a, and Orally Active Insecticidal Peptide(OAIP) respectively and they are easy to express with the help of the bacteria <i>E. coli BL21 Rosetta Gami strain</i>. So, we then start to create BioBricks of these three toxins which we have obtained the nucleotide sequences. We use a strong T7 promoter that is suitable with the strain of <i>E. coli</i> we chose and also powerful at expressing proteins with multiple disulfide bonds, and we add His-tag for protein purification.</p>
+
<p class="content">We have known the function of the three toxic peptides of PANTIDE, Omega-hexatoxin-Hv1a, µ-segestritoxin-Sf1a, and Orally Active Insecticidal Peptide(OAIP) respectively and they are easy to express with the help of the bacteria <i>E. coli</i> BL21 Rosetta Gami strain. So, we then start to create BioBricks of these three toxins which we have obtained the nucleotide sequences. We use a strong T7 promoter that is suitable with the strain of <i>E. coli</i> we chose and also powerful at expressing proteins with multiple disulfide bonds, and we add His-tag for protein purification.</p>
  
−
<p class="title">Pantide without lectin</p>
+
<p class="title">PANTIDE without Lectin</p>
−
<p class="content"> Pantide can be a single toxic peptide of the three toxins we chose, Hv1a, Sf1a, and OAIP respectively, which derive from the natural predators spiders. </p>
+
<p class="content"> PANTIDE can be a single toxic peptide of the three toxins we chose, Hv1a, Sf1a, and OAIP respectively, which derive from the natural predators spiders. </p>
  
 
<!----------Hv1a-------->
 
<!----------Hv1a-------->
−
<a href="#" class="biobrick"><p class="content-1">BBa_........</p></a>
+
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1974011" class="biobrick"><p class="content-1">BBa_K1974011</p></a>
  
 
<div>
 
<div>
−
       <img src="#" class="picture">
+
       <img src="https://static.igem.org/mediawiki/2016/f/f7/NCTU_FORMOSA_H1.png" class="picture">
 
</div>
 
</div>
  
 
<p class="content">
 
<p class="content">
 
T7 promoter+RBS+Hv1a+linker+His-tag+terminator
 
T7 promoter+RBS+Hv1a+linker+His-tag+terminator
−
Pantide that contains the gene coding for Hv1a linked with His-tag.
+
<br>PANTIDE that contains the gene coding for Hv1a linked with His-tag.
 
</p>
 
</p>
  
 
<!---------Sf1a----------->
 
<!---------Sf1a----------->
−
<a href="#" class="biobrick"><p class="content-1">BBa_........</p></a>
+
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1974012" class="biobrick"><p class="content-1">BBa_K1974012</p></a>
  
 
<div>
 
<div>
−
       <img src="#" class="picture">
+
       <img src="https://static.igem.org/mediawiki/2016/a/ad/NCTU_FORMOSA_S1.png" class="picture">
 
</div>
 
</div>
  
 
<p class="content">
 
<p class="content">
 
T7 promoter+RBS+Sf1a+linker+His-tag+terminator
 
T7 promoter+RBS+Sf1a+linker+His-tag+terminator
−
Pantide that contains the gene coding for Sf1a linked with His-tag.
+
<br>PANTIDE that contains the gene coding for Sf1a linked with His-tag.
 
</p>
 
</p>
  
 
<!-------OAIP------------->
 
<!-------OAIP------------->
−
<a href="#" class="biobrick"><p class="content-1">BBa_........</p></a>
+
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1974013" class="biobrick"><p class="content-1">BBa_K1974013</p></a>
  
 
<div>
 
<div>
−
       <img src="#" class="picture">
+
       <img src="https://static.igem.org/mediawiki/2016/2/21/NCTU_FORMOSA_O.png" class="picture">
 
</div>
 
</div>
  
 
<p class="content">
 
<p class="content">
 
T7 promoter+RBS+OAIP+linker+His-tag+terminator
 
T7 promoter+RBS+OAIP+linker+His-tag+terminator
−
Pantide that contains the gene coding for OAIP linked with His-tag.
+
<br>PANTIDE that contains the gene coding for OAIP linked with His-tag.
 
</p>
 
</p>
  
Line 487: Line 484:
  
  
−
<p class="title">Pantide with lectin</p>
+
<p class="title">PANTIDE with Lectin</p>
−
<p class="content"> Pantide can be a single toxic peptide of the three toxins we chose, Hv1a, Sf1a, and OAIP respectively with the addition of Galanthus nivalis agglutinin (GNA), for GNA has the function of acting as a carrier of Pantide from insect gut to the haemolymph and finally Pantide can transmit to the action site. </p>
+
<p class="content"> PANTIDE can be a single toxic peptide of the three toxins we chose, Hv1a, Sf1a, and OAIP respectively with the addition of Galanthus nivalis agglutinin (GNA), for GNA has the function of acting as a carrier of PANTIDE from insect gut to the haemolymph and finally PANTIDE can transmit to the action site. </p>
  
 
<!----------Hv1a lectin----------->
 
<!----------Hv1a lectin----------->
−
<a href="#" class="biobrick"><p class="content-1">BBa_........</p></a>
+
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1974021" class="biobrick"><p class="content-1">BBa_K1974021</p></a>
  
 
<div>
 
<div>
−
       <img src="#" class="picture">
+
       <img src="https://static.igem.org/mediawiki/2016/b/b6/NCTU_FORMOSA_HL.png" class="picture">
 
</div>
 
</div>
  
 
<p class="content">
 
<p class="content">
−
T7 promoter+RBS+Hv1a-lectin+linker+His-tag+terminator
+
T7 promoter+RBS+Hv1a+linker+lectin+linker+His-tag+terminator
−
Pantide that contains the gene coding for Hv1a and the snowdrop lectin linked with His-tag.
+
<br>PANTIDE that contains the gene coding for Hv1a and the snowdrop lectin linked with His-tag.
  
 
</p>
 
</p>
  
 
<!---------Sf1a lectin---------->
 
<!---------Sf1a lectin---------->
−
<a href="#" class="biobrick"><p class="content-1">BBa_........</p></a>
+
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1974022" class="biobrick"><p class="content-1">BBa_K1974022</p></a>
  
 
<div>
 
<div>
−
       <img src="#" class="picture">
+
       <img src="https://static.igem.org/mediawiki/2016/c/c3/NCTU_FORMOSA_SL.png" class="picture">
 
</div>
 
</div>
  
 
<p class="content">
 
<p class="content">
−
T7 promoter+RBS+Sf1a-lectin+linker+His-tag+terminator
+
T7 promoter+RBS+Sf1a+linker+lectin+linker+His-tag+terminator
−
Pantide that contains the gene coding for Sf1a and the snowdrop lectin linked with His-tag.
+
<br>PANTIDE that contains the gene coding for Sf1a and the snowdrop lectin linked with His-tag.
  
 
</p>
 
</p>
  
 
<!--------OAIP lectin------>
 
<!--------OAIP lectin------>
−
<a href="#" class="biobrick"><p class="content-1">BBa_........</p></a>
+
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1974023" class="biobrick"><p class="content-1">BBa_K1974023</p></a>
  
 
<div>
 
<div>
−
       <img src="#" class="picture">
+
       <img src="https://static.igem.org/mediawiki/2016/2/27/NCTU_FORMOSA_OL.png" class="picture">
 
</div>
 
</div>
  
 
<p class="content">
 
<p class="content">
−
T7 promoter+RBS+OAIP-lectin+linker+His-tag+terminator
+
T7 promoter+RBS+OAIP+linker+lectin+linker+His-tag+terminator
−
Pantide that contains the gene coding for OAIP and the snowdrop lectin linked with His-tag.
+
<br>PANTIDE that contains the gene coding for OAIP and the snowdrop lectin linked with His-tag.
  
 
</p>
 
</p>
Line 531: Line 528:
 
<!---------------GS linker----------------->
 
<!---------------GS linker----------------->
  
−
<p class="title">Pantide with Gs linker</p>
+
<p class="title">PANTIDE with GS Linker</p>
−
<p class="content"> In this part, to facilitate the expression of Pantide, we extended the linker with the substitution of Gs linker that is even better than the original linker for the more correcter folding of the proteins structure.</p>
+
<p class="content"> In this part, to facilitate the expression of PANTIDE, we extended the linker with the substitution of GS linker that is even better than the original linker for the more correcter folding of the proteins structure.</p>
  
 
<!-----------OAIP GS linker--------------->
 
<!-----------OAIP GS linker--------------->
−
<a href="#" class="biobrick"><p class="content-1">BBa_........</p></a>
+
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1974033" class="biobrick"><p class="content-1">BBa_K1974033</p></a>
  
 
<div>
 
<div>
−
       <img src="#" class="picture">
+
       <img src="https://static.igem.org/mediawiki/2016/d/d6/NCTU_FORMOSA_GS1.png" class="picture">
 
</div>
 
</div>
  
 
<p class="content">
 
<p class="content">
−
T7 promoter+RBS+Hv1a-lectin+Gs linker+His-tag+terminator
+
T7 promoter+RBS+Hv1a+GS linker+lectin+linker+His-tag+terminator
−
Pantide that contains the gene coding for Hv1a and the snowdrop lectin. And improve the correct folding of Pantide by substituting with Gs linker linked with His-tag.
+
<br>PANTIDE that contains the gene coding for Hv1a and the snowdrop lectin. And improve the correct folding of PANTIDE by substituting with GS linker linked with His-tag.
  
  
Line 552: Line 549:
 
</div>
 
</div>
 
</section>
 
</section>
 +
 +
 +
 +
 +
 +
 +
<!--JS-->
 +
 +
<script>
 +
 +
var $document = $( document.body );
 +
var $revealBar = $( '.red-container' );
 +
var wrapperHeight = $document.height();
 +
var stepDistance = 101;
 +
var documentHeight = window.innerHeight;
 +
var positions = [];
 +
var heights = [];
 +
var elements = $( '.title' );
 +
 +
// Cache heights and positions
 +
for( var i = 0; i < elements.length; i++ ) {
 +
  var $element = $(elements[i]);
 +
  var height = $element.offset().top + documentHeight;
 +
  if (height > wrapperHeight) {
 +
    height = wrapperHeight;
 +
  }
 +
  positions.push(height);
 +
  heights.push($element.height());
 +
}
 +
 +
var $nodes =  $( '.node' );
 +
 +
// Should totally be debounced /w animation frame. I know,
 +
// this whole thing is slightly inefficient.
 +
// And magic numbers too!
 +
$( document ).scroll( function() {
 +
 +
  nodeTop = $document.scrollTop() + documentHeight + 1;
 +
  var current = 0;
 +
 
 +
  // Active/non active states
 +
  for( var i = 0; i < positions.length; i++ ) {
 +
    if(nodeTop >= positions[i]-80) {
 +
      current = i;
 +
      $nodes.eq( i ).addClass( 'active' );
 +
    } else {
 +
      $nodes.eq( i ).removeClass( 'active' );
 +
    }
 +
  }
 +
 
 +
 
 +
  if ( current < elements.length ) {
 +
    var nextStep = (nodeTop - positions[current]) / (positions[current + 1] - positions[current]);
 +
    var totalWidth = ( current * stepDistance ) + (nextStep * stepDistance);
 +
$revealBar.height( totalWidth+25 );
 +
  }
 +
 +
})
 +
 +
// Clicking the nodes... again, nothing special ;)
 +
$nodes.each( function( index ) {
 +
  var $node = $( this );
 +
  $node.click( function() {
 +
    $('html, body').animate({ scrollTop: (positions[ index ]-documentHeight)-70}, 1000 );
 +
  });
 +
})
 +
//scroll_relocate
 +
function sticky_relocate() {
 +
    var window_top = $(window).scrollTop();
 +
    var div_top = $('.main-img').offset().top;;
 +
    if (window_top > div_top+500) {
 +
        $('#scroll').removeClass('appear');
 +
    } else {
 +
        $('#scroll').addClass('appear');
 +
    };
 +
    if(window_top > div_top+500) {
 +
        $('#web').fadeIn(200);
 +
        $('#right').fadeIn(200);
 +
    } else {
 +
        $('#web').fadeOut(200);
 +
        $('#right').fadeOut(200);
 +
    }
 +
}
 +
 +
$(function () {
 +
    $(window).scroll(sticky_relocate);
 +
    sticky_relocate();
 +
});
 +
 +
 +
 +
$(document).ready(function(
 +
 +
){})
 +
 +
</script>
 +
  
  

Latest revision as of 03:13, 4 November 2016

Composite Parts

We have known the function of the three toxic peptides of PANTIDE, Omega-hexatoxin-Hv1a, µ-segestritoxin-Sf1a, and Orally Active Insecticidal Peptide(OAIP) respectively and they are easy to express with the help of the bacteria E. coli BL21 Rosetta Gami strain. So, we then start to create BioBricks of these three toxins which we have obtained the nucleotide sequences. We use a strong T7 promoter that is suitable with the strain of E. coli we chose and also powerful at expressing proteins with multiple disulfide bonds, and we add His-tag for protein purification.

PANTIDE without Lectin

PANTIDE can be a single toxic peptide of the three toxins we chose, Hv1a, Sf1a, and OAIP respectively, which derive from the natural predators spiders.

BBa_K1974011

T7 promoter+RBS+Hv1a+linker+His-tag+terminator
PANTIDE that contains the gene coding for Hv1a linked with His-tag.

BBa_K1974012

T7 promoter+RBS+Sf1a+linker+His-tag+terminator
PANTIDE that contains the gene coding for Sf1a linked with His-tag.

BBa_K1974013

T7 promoter+RBS+OAIP+linker+His-tag+terminator
PANTIDE that contains the gene coding for OAIP linked with His-tag.

PANTIDE with Lectin

PANTIDE can be a single toxic peptide of the three toxins we chose, Hv1a, Sf1a, and OAIP respectively with the addition of Galanthus nivalis agglutinin (GNA), for GNA has the function of acting as a carrier of PANTIDE from insect gut to the haemolymph and finally PANTIDE can transmit to the action site.

BBa_K1974021

T7 promoter+RBS+Hv1a+linker+lectin+linker+His-tag+terminator
PANTIDE that contains the gene coding for Hv1a and the snowdrop lectin linked with His-tag.

BBa_K1974022

T7 promoter+RBS+Sf1a+linker+lectin+linker+His-tag+terminator
PANTIDE that contains the gene coding for Sf1a and the snowdrop lectin linked with His-tag.

BBa_K1974023

T7 promoter+RBS+OAIP+linker+lectin+linker+His-tag+terminator
PANTIDE that contains the gene coding for OAIP and the snowdrop lectin linked with His-tag.

PANTIDE with GS Linker

In this part, to facilitate the expression of PANTIDE, we extended the linker with the substitution of GS linker that is even better than the original linker for the more correcter folding of the proteins structure.

BBa_K1974033

T7 promoter+RBS+Hv1a+GS linker+lectin+linker+His-tag+terminator
PANTIDE that contains the gene coding for Hv1a and the snowdrop lectin. And improve the correct folding of PANTIDE by substituting with GS linker linked with His-tag.