Difference between revisions of "Team:SCSU-New Haven/Safety"

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<h1 class="headers">Safety</h1>
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<h2 class="headers">How will this experiment work?</h2>
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Tuberculosis is caused by an infection of Mycobacterium Tuberculosis and damages the lungs in an individual. Volatile Organic Compounds or VOCs are emitted from an infected person’s lungs as aerosols. These gasses contain certain compounds which can be indicated with a modified version of Escherichia coli or E. coli along with a breathalyzer. When the E. coli cells locate the tested compounds, a blue dye will be expressed which will demonstrate a rapid detection of Tuberculosis.
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<h2>Potential risks or hazards</h2>
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The tested strain of E. coli is classified as a Risk Group 1 level organism, meaning it poses very little to no threat at all when handled by humans. Using appropriate BSL (Biosafety Level) 1 techniques instructed by the PI, Dr. Nicholas Edgington, proper laboratory techniques and etiquette were used throughout the entire experiment such as:
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<p>Wearing clothing that covers exposed areas of the skin, this excludes the use of opened toe shoes <br> or any type of summer clothing that is unacceptable in a lab setting.</p>
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<p>Properly washing one’s hands before and after working in the lab and wiping down used lab benches with 70% EtOH.</p>
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<p>Having access to and using personal protective equipment (PPE). This includes safety glasses<br> or goggles, gloves, lab coats, as well as proper disposal containers for lab equipment.</p>
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<p>Preventing the use of food or drinks at any time in the lab.</p>
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<p>Using the appropriate lab tools on the bench and removing them when they were no longer needed.</p>
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<h2 class="headers">Chemical Risks</h2>
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Ethidium bromide, used for gel staining is a known mutagen. Gels and TBE buffer containing ethidium bromide were disposed of in separate containers from other chemicals.
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<h2 class="headers">Other Safety Matters</h2>
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<h3>Will this experiment be used outside of the lab setting?</h3>
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The details or data could be used in other laboratories but any cells, reagents, or any physical biomaterial will not be used outside of the university lab.
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<p>Please visit <a href="https://2016.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<h5>Safe Project Design</h5>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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<li>Choosing a non-pathogenic chassis</li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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<h5>Safe Lab Work</h5>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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<h5>Safe Shipment</h5>
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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Latest revision as of 23:56, 30 November 2016

SCSU IGEM SCSU IGEM

Safety

How will this experiment work?

Tuberculosis is caused by an infection of Mycobacterium Tuberculosis and damages the lungs in an individual. Volatile Organic Compounds or VOCs are emitted from an infected person’s lungs as aerosols. These gasses contain certain compounds which can be indicated with a modified version of Escherichia coli or E. coli along with a breathalyzer. When the E. coli cells locate the tested compounds, a blue dye will be expressed which will demonstrate a rapid detection of Tuberculosis.

Potential risks or hazards

The tested strain of E. coli is classified as a Risk Group 1 level organism, meaning it poses very little to no threat at all when handled by humans. Using appropriate BSL (Biosafety Level) 1 techniques instructed by the PI, Dr. Nicholas Edgington, proper laboratory techniques and etiquette were used throughout the entire experiment such as:

Wearing clothing that covers exposed areas of the skin, this excludes the use of opened toe shoes
or any type of summer clothing that is unacceptable in a lab setting.

Properly washing one’s hands before and after working in the lab and wiping down used lab benches with 70% EtOH.

Having access to and using personal protective equipment (PPE). This includes safety glasses
or goggles, gloves, lab coats, as well as proper disposal containers for lab equipment.

Preventing the use of food or drinks at any time in the lab.

Using the appropriate lab tools on the bench and removing them when they were no longer needed.

Chemical Risks

Ethidium bromide, used for gel staining is a known mutagen. Gels and TBE buffer containing ethidium bromide were disposed of in separate containers from other chemicals.

Other Safety Matters

Will this experiment be used outside of the lab setting?

The details or data could be used in other laboratories but any cells, reagents, or any physical biomaterial will not be used outside of the university lab.