Difference between revisions of "Team:SCSU-New Haven/Safety"

 
(10 intermediate revisions by the same user not shown)
Line 1: Line 1:
 
{{SCSU-New_Haven/Head}}
 
{{SCSU-New_Haven/Head}}
 +
{{SCSU-New_Haven/Navbar}}
 
<html>
 
<html>
 
<head>
 
<head>
−
<meta charset="UTF-8">
+
 
−
<title>SCSU iGEM</title>
+
−
<link rel="stylesheet" type="text/css"
+
−
href="https://2016.igem.org/Template:SCSU-New_Haven/dev?action=raw&ctype=text/css">
+
−
<link rel="stylesheet" type="text/css"
+
−
href="https://2016.igem.org/Template:SCSU-New_Haven/bootstrap?action=raw&ctype=text/css">
+
−
<!-- <link rel="stylesheet" type="text/css" href="https://2016.igem.org/Template:SCSU-New_Haven/Scroll?action=raw&ctype=text/css"> -->
+
 
</head>
 
</head>
 
<body>
 
<body>
−
<div id="page-content">
+
 +
<div id="page">
 
<div class="text-center" id="safety">
 
<div class="text-center" id="safety">
−
<div class="safety-div bckgnd_image" id="theme1">
+
<div class="safety-div bckgnd_image" id="safety_1">
−
<h1 class="headers">Page Title: Safety</h1>
+
<div class="container">
−
<h2 class="headers">How will this experiment work?</h2>
+
<h1 class="headers">Safety</h1>
−
<p>
+
<h2 class="headers">How will this experiment work?</h2>
−
Tuberculosis is caused by an infection of Mycobacterium Tuberculosis and damages the lungs in an individual. Volatile Organic Compounds or VOCs are emitted from an infected person’s lungs as aerosols. These gasses contain certain compounds which can be indicated with a modified version of Escherichia coli or E. coli along with a breathalyzer. When the E. coli cells locate the tested compounds, a blue dye will be expressed which will demonstrate a rapid detection of Tuberculosis.
+
<p>
−
<b>Are there any risks or potential hazards involved in the experiment? If so, what actions will the lab be taking in order to prevent or minimize those risks?</b>
+
Tuberculosis is caused by an infection of Mycobacterium Tuberculosis and damages the lungs in an individual. Volatile Organic Compounds or VOCs are emitted from an infected person’s lungs as aerosols. These gasses contain certain compounds which can be indicated with a modified version of Escherichia coli or E. coli along with a breathalyzer. When the E. coli cells locate the tested compounds, a blue dye will be expressed which will demonstrate a rapid detection of Tuberculosis.
−
</p>
+
 +
</p>
 +
</div>
 
</div>
 
</div>
−
<div class="safety-div bckgnd_image" id="theme2">
+
<div class="safety-div bckgnd_image" id="safety_2">
−
<h2 class="headers">General Intro</h2>
+
<div class="container">
−
<p>
+
<h2>Potential risks or hazards</h2>
−
The tested strain of E. coli is classified as a Risk Group 1 level organism, meaning it poses very little to no threat at all when handled by humans. Using appropriate BSL (Biosafety Level) 1 techniques instructed by the PI, Dr. Nicholas Edgington, proper laboratory techniques and etiquette were used throughout the entire experiment such as:
+
<p>
−
<ul>
+
The tested strain of E. coli is classified as a Risk Group 1 level organism, meaning it poses very little to no threat at all when handled by humans. Using appropriate BSL (Biosafety Level) 1 techniques instructed by the PI, Dr. Nicholas Edgington, proper laboratory techniques and etiquette were used throughout the entire experiment such as:
−
<li>Wearing clothing that covers exposed areas of the skin, this excludes the use of opened toe shoes or any type of summer clothing that is unacceptable in a lab setting.</li>
+
</p>
−
<li>Properly washing one’s hands before and after working in the lab and wiping down used lab benches with 70% EtOH.</li>
+
<div class="container" style="margin-top:2%">
−
<li>Having access to and using personal protective equipment (PPE). This includes safety glasses or goggles, gloves, lab coats, as well as proper disposal containers for lab equipment.</li>
+
<p>Wearing clothing that covers exposed areas of the skin, this excludes the use of opened toe shoes <br> or any type of summer clothing that is unacceptable in a lab setting.</p>
−
<li>Preventing the use of food or drinks at any time in the lab.</li>
+
<p>Properly washing one’s hands before and after working in the lab and wiping down used lab benches with 70% EtOH.</p>
−
<li>Using the appropriate lab tools on the bench and removing them when they were no longer needed.</li>
+
<p>Having access to and using personal protective equipment (PPE). This includes safety glasses<br> or goggles, gloves, lab coats, as well as proper disposal containers for lab equipment.</p>
−
</ul>
+
<p>Preventing the use of food or drinks at any time in the lab.</p>
−
</p>
+
<p>Using the appropriate lab tools on the bench and removing them when they were no longer needed.</p>
 +
</div>
 +
</div>
 
</div>
 
</div>
−
<div class="safety-div bckgnd_image" id="theme3">
+
<div class="safety-div bckgnd_image" id="safety_3">
−
<h2 class="headers">Chemical Risks</h2>
+
<div class="container">
−
<p>
+
<h2 class="headers">Chemical Risks</h2>
−
Lorem Ipsum is simply dummy text of the printing and typesetting industry. Lorem Ipsum has been the industry's standard dummy text ever since the 1500s, when an unknown printer took a galley of type and scrambled it to make a type specimen book. It has survived not only five centuries, but also the leap into electronic typesetting, remaining essentially unchanged. It was popularised in the 1960s with the release of Letraset sheets containing Lorem Ipsum passages, and more recently with desktop publishing software like Aldus PageMaker including versions of Lorem Ipsum.
+
<p>
−
</p>
+
Ethidium bromide, used for gel staining is a known mutagen. Gels and TBE buffer containing ethidium bromide were disposed of in separate containers from other chemicals.  
 +
</p>
 +
</div>
 
</div>
 
</div>
−
<div class="safety-div bckgnd_image" id="theme1">
+
<div class="safety-div bckgnd_image" id="safety_4">
−
<h2 class="headers">Other Safety Matters</h2>
+
<div class="container">
−
<p>
+
<h2 class="headers">Other Safety Matters</h2>
−
Will this experiment be used outside of the lab setting? The details or data could be used in other laboratories but any cells, reagents, or any physical biomaterial will not be used outside of the university lab.
+
<h3>Will this experiment be used outside of the lab setting?</h3>
−
</p>
+
<p>
 +
The details or data could be used in other laboratories but any cells, reagents, or any physical biomaterial will not be used outside of the university lab.
 +
</p>
 +
</div>
 
</div>
 
</div>
  
 
</div>
 
</div>
−
</div>
+
</div>
−
 
+
−
+
 
</body>
 
</body>
 
</html>
 
</html>
 
{{SCSU-New_Haven/Footer}}
 
{{SCSU-New_Haven/Footer}}

Latest revision as of 23:56, 30 November 2016

SCSU IGEM SCSU IGEM

Safety

How will this experiment work?

Tuberculosis is caused by an infection of Mycobacterium Tuberculosis and damages the lungs in an individual. Volatile Organic Compounds or VOCs are emitted from an infected person’s lungs as aerosols. These gasses contain certain compounds which can be indicated with a modified version of Escherichia coli or E. coli along with a breathalyzer. When the E. coli cells locate the tested compounds, a blue dye will be expressed which will demonstrate a rapid detection of Tuberculosis.

Potential risks or hazards

The tested strain of E. coli is classified as a Risk Group 1 level organism, meaning it poses very little to no threat at all when handled by humans. Using appropriate BSL (Biosafety Level) 1 techniques instructed by the PI, Dr. Nicholas Edgington, proper laboratory techniques and etiquette were used throughout the entire experiment such as:

Wearing clothing that covers exposed areas of the skin, this excludes the use of opened toe shoes
or any type of summer clothing that is unacceptable in a lab setting.

Properly washing one’s hands before and after working in the lab and wiping down used lab benches with 70% EtOH.

Having access to and using personal protective equipment (PPE). This includes safety glasses
or goggles, gloves, lab coats, as well as proper disposal containers for lab equipment.

Preventing the use of food or drinks at any time in the lab.

Using the appropriate lab tools on the bench and removing them when they were no longer needed.

Chemical Risks

Ethidium bromide, used for gel staining is a known mutagen. Gels and TBE buffer containing ethidium bromide were disposed of in separate containers from other chemicals.

Other Safety Matters

Will this experiment be used outside of the lab setting?

The details or data could be used in other laboratories but any cells, reagents, or any physical biomaterial will not be used outside of the university lab.