Difference between revisions of "Team:NTU-Singapore/Attributions"

 
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{{NTU-Singapore}}
 
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2016.igem.org/Judging/Medals">Attributions bronze criterion</a>. </p>
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<a href="https://2016.igem.org/Team:NTU-Singapore" class="dropdown-toggle" data-toggle="dropdown"><i class="fa fa-2x fa-home"></i> <span class="navbar-name"> NTU-SG</span> </a>
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</li>
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                <li class="dropdown">
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                    <a href="https://2016.igem.org/Team:NTU-Singapore/Collaborations" class="dropdown-toggle" data-toggle="dropdown"><i class="fa fa-2x fa-globe"></i><span class="navbar-name"> Collaboration</span></a>
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<ul class="dropdown-menu dropdown-menu-project" style="margin: 0px;  left: -40px;">
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<a href="https://2016.igem.org/Team:NTU-Singapore/Interlab"><i class="fa fa-2x fa-balance-scale"></i><span style="top: -34px; float: left; left: 50px;"class="navbar-name"> Interlab Measurement </span> </a>
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<a href="https://2016.igem.org/Team:NTU-Singapore/Description"  style="top: -20px; position: relative;"><i class="fa fa-fw fa-2x fa-book"></i><span style="left: 9px;" class="navbar-name"> Description</span> </a>
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</li>
  
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<li>
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<a href="https://2016.igem.org/Team:NTU-Singapore/Proof" style="height: 49px;"><i class="fa fa-2x  fa-bar-chart" style="float: left;"></i><span style="width: 96px;
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left: 10px;
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top: -6px;" class="navbar-name"> Proof of concept </span></a>
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<a href="https://2016.igem.org/Team:NTU-Singapore/Demonstrate"><i class="fa fa-2x  fa-space-shuttle"></i><span style="left: 9px;" class="navbar-name"> Demonstrate</span> </a>
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</ul>
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<li class="dropdown">
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<a href="#" class="dropdown-toggle" data-toggle="dropdown"><i class="fa fa-2x fa-gears"></i><span class="navbar-name"> Parts</span>  <b class="caret"></b></a>
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<div class="drop-menu">
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<ul class="dropdown-menu" style="margin: 0px; left: -42px;">
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<li>
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<a href="https://2016.igem.org/Team:NTU-Singapore/Basic_Part"><i class="fa fa-fw fa-2x fa-gear"></i><span style="left: 8px;"class="navbar-name"> Basic Parts</span> </a>
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</li>
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<li>
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<a style="height: 49px" href="https://2016.igem.org/Team:NTU-Singapore/Composite_Part"><i style="float: left;" class="fa fa-fw fa-2x fa-briefcase"></i><span style="    float: left;
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width: 96px;
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top: -6px;
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left: 13px;" class="navbar-name"> CRISPR<span style="text-transform: lowercase;">y</span> Collection</span> </a>
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</li>
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</ul>
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</div>
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<li class="dropdown">
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<a href="#" class="dropdown-toggle" data-toggle="dropdown"><i class="fa fa-2x fa-key"></i><span class="navbar-name"> Team</span>  <b class="caret"></b></a>
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<div class="drop-menu">
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<ul class="dropdown-menu" style="margin: 0px; left: -50px;">
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<li>
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<a href="https://2016.igem.org/Team:NTU-Singapore/Team"><i class="fa fa-2x fa-flag-checkered" aria-hidden="true"></i><span style="left: 13px;"class="navbar-name">Adventurers</span> </a>
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</li>
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<li>
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<a href="https://2016.igem.org/Team:NTU-Singapore/Attributions"><i class="fa fa-2x fa-key" aria-hidden="true"></i><span style="left: 13px;" class="navbar-name">Attribution</span> </a>
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</li>
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<li>
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<a style="height: 49px" href="https://2016.igem.org/Team:NTU-Singapore/Notebook"><i class="fa fa-2x fa-file-text-o" aria-hidden="true"></i>
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<span style=" left: 13px;" class="navbar-name">Notebook</span> </a>
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</ul>
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<a href="#" class="dropdown-toggle" data-toggle="dropdown"><i class="fa fa-2x  fa-group"></i><span class="navbar-name"> Human Practice</span>  <b class="caret"></b></a>
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<div class="drop-menu">
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<ul class="dropdown-menu dropdown-menu-humanprac" style="margin: 0px">
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<li>
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<a href="https://2016.igem.org/Team:NTU-Singapore/HP/Silver"><i class="fa fa-fw fa-2x fa-user"></i><span class="navbar-name"> Silver</span> </a>
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</li>
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<li>
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<a href="https://2016.igem.org/Team:NTU-Singapore/HP/Gold"><i class="fa fa-fw fa-2x fa-envelope"></i><span class="navbar-name"> Gold</span> </a>
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</li>
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</ul>
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<p> Each team must clearly attribute work done by the student team members on this page. The team must distinguish work done by the students from work done by others, including the host labs, advisors, instructors, and individuals not on the team roster. </p>
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            </ul>
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        </nav>
  
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        <div id="page-wrapper" >
  
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<div class="clear"></div>
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                <!-- Main jumbotron for a primary marketing message or call to action -->
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                <div class="jumbotron content-page" style="font-family: 'Just Me Again Down Here', cursive; padding-top: 140px; margin-bottom: -10px;">
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<h1 style="font-size: 70px;"><span style="color: #0089A7; ">Thanks</span> for the help and guidance!</h1>
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<p style="font-family: 'Annie Use Your Telescope', cursive; font-size: 26px; line-height: 51px;">We are very gratefull that we have these super helpful labmates that are willing to spend time teaching us! They are the key to our success! :)</p>
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</div>
  
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</div>
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<div class="page-header">
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<h1>Advisors</h1>
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</div>
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<div class="well" style="color: black">
  
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<div class="column half_size">
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                                <h2>Asst. Prof Tan Meng How</h2>
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<h5> Why is this page needed? </h5>
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                                <p>Assembled the team and met with all the team members every week to provide guidance and set research directions. Designed gRNAs for project Evaluation and primers for genomic PCR.</p>  
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<p>The Attribution requirement helps the judges know what you did yourselves and what you had help with. We don't mind if you get help with difficult or complex techniques, but you must report what work your team did and what work was done by others.</p>
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<p>
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For example, you might choose to work with an animal model during your project. Working with animals requires getting a license and applying far in advance to conduct certain experiments in many countries. This is difficult to achieve during the course of a summer, but much easier if you can work with a postdoc or PI who has the right licenses.</p>
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</div>
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                              <h2>Dr. Teh Min Yan</h2>
 +
                                <p>Mentored Fanxiang and Huanwen for Project Evolution. Taught us mammalian tissue culture</p>
  
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<div class="column half_size">
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                                <h2>Dr. Yuan Ming</h2>
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<h5> What should this page have?</h5>
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                                <p>In-charge of Project Evaluation</p>
  
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<ul>
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                              <h2>Liu Kaiwen Ivy</h2>
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<li>General Support</li>
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                                <p>Mentored us on Project Evaluation, taught us experiments involved in T7E assay.</p>  
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<li>Project support and advice</li>
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<li>Fundraising help and advice</li>
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<li>Lab support</li>
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<li>Difficult technique support</li>
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<li>Project advisor support</li>
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<li>Wiki support</li>
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<li>Presentation coaching</li>
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<li>Human Practices support</li>
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<li> Thanks and acknowledgements for all other people involved in helping make a successful iGEM team</li>
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</ul>
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</div>
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<div class="clear"></div>
 
  
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<div class="column half_size">
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<div class="highlight">
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<div class="page-header">
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<h5> Can we base our project on a previous one? </h5>
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<h1>Adventurers</h1>
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<p>Yes! You can have a project based on a previous team, or based on someone else's idea, <b>as long as you state this fact very clearly and give credit for the original project.</b> </p>
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</div>
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</div>
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<div class="well" style="color: black">
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</div>
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                              <h2>Ooi Kean Hean</h2>
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                                <p>Student leader of the team. Designed the wiki, Cas9 models and exhibition items. In charge on Project Truncation, analysed crystal structure of Sp-dCas9 for determination of truncations, Gibson cloning of truncation regions, maintanence of HEK293FT cells and preparation for transfection, did flow cytometry and analysis of the results for Project Truncation. </p>  
 +
                              <h2>Yong Jing Yen</h2>
 +
                                <p>Responsible for submission of parts and experiments involved in collaboration. Designed Cas9 models, poster and exhibition items. Cloned constructs for Project Evaluation and carried out T7E assay, transfection of HEK293 cells, FACS preparation and genomic PCR</p>  
  
 +
                              <h2>Liu Zhehui</h2>
 +
                                <p>Designed the brochure for our exhibition. Designed the wiki. Photographer of our team. Helped out in Project Truncation, i.e. Gibson cloning of truncation regions, preparation of HEK293FT cells for transfection, flow cytometry, and derived truncation regions in the PI domain. Cloned constructs for Project Evaluation and carried out T7E assay and genomic PCR</p>
 +
                              <h2>Li Fanxiang</h2>
 +
                                <p>Carried out all the assays involved in Project Evolution. Photographer of our team. Drew all the fantastic picture of us!</p>
 +
                              <h2>Mu Huanwen</h2>
 +
                                <p>Carried out all the assays involved in Project Evolution and testing the mutant Cas9 in HEK 293 cells</p>
 +
                              <h2>Zhang Junyi</h2>
 +
                                <p>Carried out experiments of Project Evalutiaon like T7E assay, transfection of HEK293 cells, FACS preparation, genomic PCR, and Surveyor Assay.</p>
 +
                             
 +
                             
 +
                              <h2>Shaun Lim Wen Zheng</h2>
 +
                                <p>Carried out experiments of Project Evalutiaon like T7E assay, transfection of HEK293 cells, FACS preparation, genomic PCR, and Surveyor Assay</p>
  
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<div class="column half_size">
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<h2>NORFALA-ALIAH BINTE SUTRISNOH</h2>
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                                <p>Carried out experiments of Project Evalutiaon like T7E assay, transfection of HEK293 cells, FACS preparation, genomic PCR, Surveyor Assay</p>  
  
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<h5>Inspiration</h5>
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<h2>YEO HWEE TING</h2>
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<p>Take a look at what other teams have done:</p>
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                                <p>Carried out experiments of Project Evalutiaon like T7E assay, transfection of HEK293 cells, FACS preparation, genomic PCR, Surveyor Assay</p>  
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<ul>
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<li><a href="https://2011.igem.org/Team:Imperial_College_London/Team">2011 Imperial College London</a> (scroll to the bottom)</li>
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<li><a href="https://2014.igem.org/Team:Exeter/Attributions">2014 Exeter </a></li>
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<li><a href="https://2014.igem.org/Team:Melbourne/Attributions">2014 Melbourne </a></li>
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<li><a href="https://2014.igem.org/Team:Valencia_Biocampus/Attributions">2014 Valencia Biocampus</a></li>
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</ul>
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</div>
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                              <h2>Wong Ming Jie</h2>
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                                <p>Helped out during our exhibition</p>
 +
                            <h2>Bui Thuy Thien</h2>
 +
                                <p>Helped out during our exhibition</p>
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                              <h2>Jenndy</h2>
 +
                                <p>Helped out during our exhibition</p>  
  
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<h1>3D printing support</h1>
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</div>
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<div class="well" style="color: black">
  
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<h5>Team training and Project start</h5>
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                                <h2>Dr. Bai Jiaming, A*STAR SIMTECH</h2>
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<p>Tell us if your institution teaches an iGEM or synthetic biology class and when you started your project:</p>
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                                <p>Dr. Bai was kind enought to help us print out our 3D models with the MakerBotX</p>
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<ul>
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<li>Does your institution teach an iGEM or synthetic biology course?</li>
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<li>When did you start this course?</li>
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<li>Are the syllabus and course materials freely available online?</li>
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<li>When did you start your brainstorming?</li>
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<li>When did you start in the lab?</li>
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<li>When did you start working on  your project?</li>
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</ul>
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                        <div class="page-header">
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<h1>FACS and Flow cytometry support</h1>
 +
</div>
 +
<div class="well" style="color: black">
  
 +
                                <h2>Dr. Abdul Rashid Bin Mohammad Muzaki</h2>
 +
                                <p>Rashid is the Flow-facility Assistant Manager who was very kind to teach us how to operate the FACSDiva software and the Fortessa X20 flow cytometry machine.</p>
  
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</div>
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<div class="page-header" style="height: 400px; width: 1182px; margin: auto;">
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<h1 style="font-family: 'Just Me Again Down Here', cursive; font-size: 50px;">Last but not least, our sponsors!</h1>
  
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<img class="content-img" src="http://www.ntu.edu.sg/AboutNTU/CorporateInfo/CorporateIdentity/PublishingImages/ntuchineselogo.jpg" style="width: 548px; height: 148px; box-shadow: none; z-index: -1; position: relative; top: -10px;/* float: left; */">
  
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<br>
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<img class="content-img" src="https://www.a-star.edu.sg/Portals/81/logo_astar.jpg" style="width: 238px; height: 128px; box-shadow: none; z-index: -1; position: relative; top: -10px; margin-right: 20px;float: left">
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<img class="content-img" src="http://www.neb-online.de/wp-content/uploads/2015/04/NEB_Logo.png" style="width: 248px; height: 128px; box-shadow: none; z-index: -1; position: relative; top: -15px; margin-right: 20px;float: left">
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<img class="content-img" src="https://static.igem.org/mediawiki/2012/2/22/IDT-Logo.jpg" style="width: 298px; height: 158px; box-shadow: none; z-index: -1; position: relative; top: -15px; margin-right: 20px;float: left">
 +
<img class="content-img" src="http://www.scitific.com/sites/default/files/Axil_logo.jpg" style="width: 338px; height: 168px; box-shadow: none; z-index: -1; position: relative; top: -30px; float: left">
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Latest revision as of 17:47, 1 December 2016

NTU-Singapore

Thanks for the help and guidance!

We are very gratefull that we have these super helpful labmates that are willing to spend time teaching us! They are the key to our success! :)

Advisors

Asst. Prof Tan Meng How

Assembled the team and met with all the team members every week to provide guidance and set research directions. Designed gRNAs for project Evaluation and primers for genomic PCR.

Dr. Teh Min Yan

Mentored Fanxiang and Huanwen for Project Evolution. Taught us mammalian tissue culture

Dr. Yuan Ming

In-charge of Project Evaluation

Liu Kaiwen Ivy

Mentored us on Project Evaluation, taught us experiments involved in T7E assay.

Adventurers

Ooi Kean Hean

Student leader of the team. Designed the wiki, Cas9 models and exhibition items. In charge on Project Truncation, analysed crystal structure of Sp-dCas9 for determination of truncations, Gibson cloning of truncation regions, maintanence of HEK293FT cells and preparation for transfection, did flow cytometry and analysis of the results for Project Truncation.

Yong Jing Yen

Responsible for submission of parts and experiments involved in collaboration. Designed Cas9 models, poster and exhibition items. Cloned constructs for Project Evaluation and carried out T7E assay, transfection of HEK293 cells, FACS preparation and genomic PCR

Liu Zhehui

Designed the brochure for our exhibition. Designed the wiki. Photographer of our team. Helped out in Project Truncation, i.e. Gibson cloning of truncation regions, preparation of HEK293FT cells for transfection, flow cytometry, and derived truncation regions in the PI domain. Cloned constructs for Project Evaluation and carried out T7E assay and genomic PCR

Li Fanxiang

Carried out all the assays involved in Project Evolution. Photographer of our team. Drew all the fantastic picture of us!

Mu Huanwen

Carried out all the assays involved in Project Evolution and testing the mutant Cas9 in HEK 293 cells

Zhang Junyi

Carried out experiments of Project Evalutiaon like T7E assay, transfection of HEK293 cells, FACS preparation, genomic PCR, and Surveyor Assay.

Shaun Lim Wen Zheng

Carried out experiments of Project Evalutiaon like T7E assay, transfection of HEK293 cells, FACS preparation, genomic PCR, and Surveyor Assay

NORFALA-ALIAH BINTE SUTRISNOH

Carried out experiments of Project Evalutiaon like T7E assay, transfection of HEK293 cells, FACS preparation, genomic PCR, Surveyor Assay

YEO HWEE TING

Carried out experiments of Project Evalutiaon like T7E assay, transfection of HEK293 cells, FACS preparation, genomic PCR, Surveyor Assay

Wong Ming Jie

Helped out during our exhibition

Bui Thuy Thien

Helped out during our exhibition

Jenndy

Helped out during our exhibition

3D printing support

Dr. Bai Jiaming, A*STAR SIMTECH

Dr. Bai was kind enought to help us print out our 3D models with the MakerBotX

FACS and Flow cytometry support

Dr. Abdul Rashid Bin Mohammad Muzaki

Rashid is the Flow-facility Assistant Manager who was very kind to teach us how to operate the FACSDiva software and the Fortessa X20 flow cytometry machine.

Last but not least, our sponsors!