Difference between revisions of "Team:ShanghaitechChina"

 
(91 intermediate revisions by 6 users not shown)
Line 12: Line 12:
 
margin:0 auto;
 
margin:0 auto;
 
padding:0;
 
padding:0;
 +
        background:url("https://static.igem.org/mediawiki/2016/1/12/Bubbles_back.png"),url("https://static.igem.org/mediawiki/2016/2/23/Bubbles3.png"),url("https://static.igem.org/mediawiki/2016/e/e1/Back3.png") repeat fixed;
 +
        font-size:100%;
 
          
 
          
 
}
 
}
 
body,td,th {
 
body,td,th {
font-family: Arial, Helvetica, sans-serif;
+
font-family: Arial, Helvetica, sans-serif,Britannic;
 
}
 
}
  
Line 51: Line 53:
 
margin:0;
 
margin:0;
 
overflow-x:hidden;
 
overflow-x:hidden;
    background:url("./images/back4.png"),url("./images/bubbles2.png"),url("./images/bubbles3.png") repeat fixed;
+
       
 
}
 
}
 
 
.skrollable {
 
.skrollable {
 
/*
 
/*
Line 89: Line 90:
 
     background-color: rgba(240,240,240,0.85);
 
     background-color: rgba(240,240,240,0.85);
 
     width:100%;
 
     width:100%;
     font-size: 18px;  
+
     font-size: 22px;  
 
     padding: 10px 30px;
 
     padding: 10px 30px;
 
}
 
}
Line 98: Line 99:
 
}
 
}
 
.introimg {
 
.introimg {
     width:85%;
+
     width:82%;
     margin-left:8%;
+
     margin-left:9%;
 
     border-radius: 10px 10px 10px 10px;
 
     border-radius: 10px 10px 10px 10px;
 
}
 
}
 
.videocontainer {
 
.videocontainer {
 
width:100%;
 
width:100%;
height:100%;
+
height:105%;
margin-top:10%
+
margin-top:10%;
 +
color:white;
 
}
 
}
.bigtext {
+
.content2 {
width:60%;
+
    background-color: rgba(255,255,255,0.85);
margin-left:20%;
+
    width:82%;
font-size:16px;
+
    margin-left:9%;
margin-top:2%;
+
    border: 1px solid #cccccc;
 +
    border-radius: 10px;
 +
    box-shadow: 1px 1px 3px rgba(0, 0, 0, 0.35);
 +
    color: #3d3d3d;
 +
    font-size: 22px;  
 +
    padding: 10px 10px 10px 10px;
 
}
 
}
 
</style>
 
</style>
Line 126: Line 133:
 
<body data-0="background-position:0px 0px;" data-10000="background-position:-1000px -20000px;">
 
<body data-0="background-position:0px 0px;" data-10000="background-position:-1000px -20000px;">
 
</div></div></div></div></div>
 
</div></div></div></div></div>
<div id="skrollr-body">
+
<nav class="navbar navbar-inverse navbar-fixed-top" style="margin-top:10px;text-align:center">
<nav class="navbar navbar-inverse navbar-fixed-top">
+
 
     <div class="container-fluid">
 
     <div class="container-fluid">
  
Line 136: Line 142:
 
                 <span class="icon-bar"></span>
 
                 <span class="icon-bar"></span>
 
             </button>
 
             </button>
                  <a href="https://2016.igem.org/Team:ShanghaitechChina" class="navbar-brand" style="font-size:28px;margin-top:3%">Solar Hunter</a>
+
            <a href="https://2016.igem.org/Team:ShanghaitechChina" class="navbar-brand" style="font-size:28px;margin-top:2%">Solar Hunter</a>
 
         </div>
 
         </div>
  
 
         <!-- Menu Items -->
 
         <!-- Menu Items -->
 
         <div class="collapse navbar-collapse" id="mainNavBar">
 
         <div class="collapse navbar-collapse" id="mainNavBar">
                 <ul class="nav navbar-nav">
+
                 <ul class="nav navbar-nav navbar-right">
 
                     <li><a href="https://2016.igem.org/Team:ShanghaitechChina" style="font-size:20px;"> </a></li>
 
                     <li><a href="https://2016.igem.org/Team:ShanghaitechChina" style="font-size:20px;"> </a></li>
 
                     <li><a href="https://2016.igem.org/Team:ShanghaitechChina" style="font-size:20px;">Home</a></li>
 
                     <li><a href="https://2016.igem.org/Team:ShanghaitechChina" style="font-size:20px;">Home</a></li>
 
                     <li class="dropdown"><a href="#" class="dropdown-toggle" data-toggle="dropdown" style="font-size:20px;">Team<span class="caret"></span></a>
 
                     <li class="dropdown"><a href="#" class="dropdown-toggle" data-toggle="dropdown" style="font-size:20px;">Team<span class="caret"></span></a>
 
                     <ul class="dropdown-menu" style="margin-left:0;">
 
                     <ul class="dropdown-menu" style="margin-left:0;">
                     <li><a href="https://2016.igem.org/Team:ShanghaitechChina/Team" style="font-size:16px;">Members</a></li>
+
                     <li><a href="https://2016.igem.org/Team:ShanghaitechChina/Team" style="font-size:20px;">Members</a></li>
                     <li><a href="https://2016.igem.org/Team:ShanghaitechChina/Attributions" style="font-size:16px;">Attributions</a></li>
+
                     <li><a href="https://2016.igem.org/Team:ShanghaitechChina/Attributions" style="font-size:20px;">Attributions</a></li>
 
                     </ul></li>
 
                     </ul></li>
 
                     <li class="dropdown"><a href="#" class="dropdown-toggle" data-toggle="dropdown" style="font-size:20px;  ">Project<span class="caret"></span></a>
 
                     <li class="dropdown"><a href="#" class="dropdown-toggle" data-toggle="dropdown" style="font-size:20px;  ">Project<span class="caret"></span></a>
 
                     <ul class="dropdown-menu" style="margin-left:0;">
 
                     <ul class="dropdown-menu" style="margin-left:0;">
                     <li><a href="https://2016.igem.org/Team:ShanghaitechChina/IBS" style="font-size:16px;">Integrative Biohydrogen System</a></li>
+
                     <li><a href="https://2016.igem.org/Team:ShanghaitechChina/IBS" style="font-size:20px;">Integrative Biohydrogen System</a></li>
                     <li><a href="https://2016.igem.org/Team:ShanghaitechChina/Nanomaterials" style="font-size:14px;">Semiconductor Nanomaterials</a></li>
+
                     <li><a href="https://2016.igem.org/Team:ShanghaitechChina/Nanomaterials" style="font-size:18px;">Semiconductor Nanomaterials</a></li>
                     <li><a href="https://2016.igem.org/Team:ShanghaitechChina/Biofilm" style="font-size:14px;">Engineered Biofilms</a></li>
+
                     <li><a href="https://2016.igem.org/Team:ShanghaitechChina/Biofilm" style="font-size:18px;">Engineered Biofilms</a></li>
                     <li><a href="https://2016.igem.org/Team:ShanghaitechChina/Hydrogen" style="font-size:14px;">Hydrogenase Gene Clusters</a></li>
+
                     <li><a href="https://2016.igem.org/Team:ShanghaitechChina/Hydrogen" style="font-size:18px;">Hydrogenase Gene Clusters</a></li>
                    <li><a href="#abs" style="font-size:14px;">Hydrogenase Gene Clusters</a></li>
+
 
                     </ul></li>
 
                     </ul></li>
 
    <li><a href="https://2016.igem.org/Team:ShanghaitechChina/Parts" style="font-size:20px;  ">Parts</a></li>
 
    <li><a href="https://2016.igem.org/Team:ShanghaitechChina/Parts" style="font-size:20px;  ">Parts</a></li>
Line 162: Line 167:
 
    <li><a href="https://2016.igem.org/Team:ShanghaitechChina/Human_Practice" style="font-size:20px;  ">Human Practice</a></li>
 
    <li><a href="https://2016.igem.org/Team:ShanghaitechChina/Human_Practice" style="font-size:20px;  ">Human Practice</a></li>
 
    <li><a href="https://2016.igem.org/Team:ShanghaitechChina/InterLab" style="font-size:20px;  ">InterLab</a></li>
 
    <li><a href="https://2016.igem.org/Team:ShanghaitechChina/InterLab" style="font-size:20px;  ">InterLab</a></li>
 +
<li><a href="https://2016.igem.org/Team:ShanghaitechChina/Safety" style="font-size:20px;  ">Safety</a></li>
 +
<li class="dropdown"><a href="#" class="dropdown-toggle" data-toggle="dropdown" style="font-size:20px;  ">Special Prize<span class="caret"></span></a><ul class="dropdown-menu" style="margin-left:0;"><li><a href="https://2016.igem.org/Team:ShanghaitechChina/Integrated_Practices" style="font-size:20px;  ">Integrated Human Practices</a></li>
 +
<li><a href="https://2016.igem.org/Team:ShanghaitechChina/Engagement" style="font-size:20px;  "> Education and Public Engagement</a></li>
 +
<li><a href="https://2016.igem.org/Team:ShanghaitechChina/Measurement" style="font-size:20px;  ">Measurement</a></li>
 +
<li><a href="https://2016.igem.org/Team:ShanghaitechChina/Design" style="font-size:20px;  ">Applied Design</a></li>
 +
</ul></li>
 
                 </ul>
 
                 </ul>
 
             </div>
 
             </div>
Line 168: Line 179:
 
     </div>
 
     </div>
 
</nav>
 
</nav>
 +
 +
<div id="skrollr-body">
 
<div data-0="opacity:1;top:3%;transform:rotate(0deg);transform-origin:0 0;" data-500="opacity:0;top:90%;transform:rotate(-90deg);">
 
<div data-0="opacity:1;top:3%;transform:rotate(0deg);transform-origin:0 0;" data-500="opacity:0;top:90%;transform:rotate(-90deg);">
 
   <img class="introimg" src="https://static.igem.org/mediawiki/2016/2/26/Intro-image.jpg">
 
   <img class="introimg" src="https://static.igem.org/mediawiki/2016/2/26/Intro-image.jpg">
 
</div>
 
</div>
<center>
+
<img class="introimg" src="https://static.igem.org/mediawiki/2016/7/71/T--ShanghaitechChina--member--bf--Home_Big_Picture.png" style="margin-top:45%;">
<img src="https://static.igem.org/mediawiki/2016/5/5d/Plan_1_V2.jpg" style="width:60%;margin-top:50%;">
+
<!--https://static.igem.org/mediawiki/2016/5/5d/Plan_1_V2.jpg-->
</center>
+
<div class="content2">
<div class="bigtext">
+
We conceived and proposed an integrative artificial photosynthesis platform, Solar Hunter, in which engineered strains, living biofilms and biofilms-interfaced semiconductor nanomaterials reside in harmony, carefully divide individuals' labor and synergistically work towards value-added products.  
Artificial photosynthesis represents a promising solution for energy issues, however, the efficiency, robustness, and scalability does not meet the requirements of industrial applications. We proposed and demonstrated a sun-powered biofilm-interfaced artificial hydrogen-producing system, Solar Hunter, that could potentially solve the issues above.
+
 
 +
 
 
</div>
 
</div>
 
<div class="content" style="margin-top:5%">
 
<div class="content" style="margin-top:5%">
<div class="row">
+
<div class="row"><center><h1><b>Integrative Biohydrogen System</b></h1></center>
 +
<div class="col-lg-5">
 +
<a href="https://2016.igem.org/Team:ShanghaitechChina/IBS"><img class="imground" src="https://static.igem.org/mediawiki/2016/c/c9/T--ShanghaitechChina--member--qlc--plan1_V5.jpg"></a>
 +
</div>
 +
<div class="col-lg-7" style="margin-top:20px" >
 +
We proposed and demonstrated a sun-powered biofilm-interfaced artificial hydrogen-producing system, Solar Hunter, that harnesses the energy from sun light. Biofilm-anchored nanorods can efficiently convert photons to electrons, which seamlessly tap into the electron chain of engineered strain carrying FeFe hydrogenase gene cluster, thereby achieving high-efficiency hydrogen production. Furthermore, the intrinsic adherence of biofilms towards various interfaces allows us to grow biofilms on easy-separation micro-beads, therefore facilitating recyclable usage of the biofilm-anchored NRs and endowing this whole system with recyclability.
 +
 
 +
</div>
 
</div>
 
</div>
 
<div class="row" style="margin-top:80px">
 
<div class="row" style="margin-top:80px">
 +
<div class="col-lg-8" style="margin-top:20px">
 +
Nanomaterials are those nanoscale objects serving as solar energy harvester. When firmly anchored onto <i>E. coli</i> biofilms through coordination chemistry, they can be easily recycled together with scalable biofilm coatings and still possess the capability to efficiently convert photons into electrons upon light exposure. The acquired electrons would tap into the electron chains of engineered strain harboring hydrogenase gene cluster, thereby fulfilling hydrogen production.
 +
</div>
 
<div class="col-lg-4">
 
<div class="col-lg-4">
<img class="imground" src="https://static.igem.org/mediawiki/2016/7/71/Biofilm_2.jpg">
+
<a href="https://2016.igem.org/Team:ShanghaitechChina/Nanomaterials"><img class="imground" src="https://static.igem.org/mediawiki/2016/9/94/T--ShanghaitechChina--member--qlc--nanomaterials.jpg"></a>
 
</div>
 
</div>
<div class="col-lg-8">
 
Artificial photosynthesis represents a promising solution for energy issues, however, the efficiency, robustness, and scalability does not meet the requirements of industrial applications. We proposed and demonstrated a sun-powered biofilm-interfaced artificial hydrogen-producing system, Solar Hunter, that could potentially solve the issues above. Biofilm-anchored nanorods can efficiently convert photons to electrons, which seamlessly tap into the electron chain of engineered strain carrying FeFe hydrogenase gene cluster, thereby achieving high-efficiency hydrogen production. Furthermore, the intrinsic adherence of biofilms towards various interfaces allows us to grow biofilms on.
 
</div>
 
 
 
</div>
 
</div>
 
<div class="row" style="margin-top:80px">
 
<div class="row" style="margin-top:80px">
<div class="col-lg-8">
 
Artificial photosynthesis represents a promising solution for energy issues, however, the efficiency, robustness, and scalability does not meet the requirements of industrial applications. We proposed and demonstrated a sun-powered biofilm-interfaced artificial hydrogen-producing system, Solar Hunter, that could potentially solve the issues above. Biofilm-anchored nanorods can efficiently convert photons to electrons, which seamlessly tap into the electron chain of engineered strain carrying FeFe hydrogenase gene cluster, thereby achieving high-efficiency hydrogen production. Furthermore, the intrinsic adherence of biofilms towards various interfaces allows us to grow biofilms on.
 
</div>
 
 
<div class="col-lg-4">
 
<div class="col-lg-4">
<img class="imground" src="https://static.igem.org/mediawiki/2016/5/5c/Hydrogenase_2.jpg">
+
<a href="https://2016.igem.org/Team:ShanghaitechChina/Biofilm"><img class="imground" src="https://static.igem.org/mediawiki/2016/a/a4/T--ShanghaitechChina--member--qlc--biofilm.jpg"></a>
 
</div>
 
</div>
 +
<div class="col-lg-8" style="margin-top:20px">
 +
Biofilms function as a platform to sustain the whole system. Biofilms can immobilize NRs firmly so that they prevent potential damage and stresses caused by free NRs, as is the case in traditional artificial photosynthesis system.  In addition,  the intrinsic adherence of biofilms towards various interfaces, allows us to grow biofilms on easy-separation micro-beads. Based on those merits, biofilm stand out by facilitating recyclable usage of the biofilm-anchored NRs and endowing this whole system with recyclability.
 +
</div>
 +
 
</div>
 
</div>
 
<div class="row" style="margin-top:80px">
 
<div class="row" style="margin-top:80px">
 +
 +
<div class="col-lg-8" style="margin-top:20px">
 +
In our sun-powered biofilm-interfaced hydrogen-producing system, hydrogenase harnessed in engineered <i>E. coli</i> are conceived to efficiently catalyze proton reduction upon receiving electrons originally donated by semiconductor nanomaterials. Electron transportation from semiconductors to hydrogenase could be bridged and facilitated by the use of mediators, methyl viologen. To achieve efficient enzymatic activities, we codon-optimized and constructed the whole hydrogenase gene clusters (from <i>Clostridium acetobutylicum</i>) by leveraging the multi-expression Acembl System.
 +
</div>
 
<div class="col-lg-4">
 
<div class="col-lg-4">
<img class="imground" src="https://static.igem.org/mediawiki/2016/6/6a/Nanomaterials_2.jpg">
+
<a href="https://2016.igem.org/Team:ShanghaitechChina/Hydrogen"><img class="imground" src="https://static.igem.org/mediawiki/2016/7/76/T--ShanghaitechChina--member--qlc--hydrogenase.jpg"></a>
 
</div>
 
</div>
<div class="col-lg-8">
 
Artificial photosynthesis represents a promising solution for energy issues, however, the efficiency, robustness, and scalability does not meet the requirements of industrial applications. We proposed and demonstrated a sun-powered biofilm-interfaced artificial hydrogen-producing system, Solar Hunter, that could potentially solve the issues above. Biofilm-anchored nanorods can efficiently convert photons to electrons, which seamlessly tap into the electron chain of engineered strain carrying FeFe hydrogenase gene cluster, thereby achieving high-efficiency hydrogen production. Furthermore, the intrinsic adherence of biofilms towards various interfaces allows us to grow biofilms on.
 
 
</div>
 
</div>
 
</div>
 
</div>
<div id="vi" class="videocontainer" data-200-bottom="background:rgba(0, 0, 0, 0.2);" data-top="background:rgba(0,0,0,1);">
+
<div id="vi" class="videocontainer" style="position-left:0" data-200-bottom="background:rgba(0, 0, 0, 0.2);" data-top="background:rgba(0,0,0,1);" style="margin-top:0">
<video data-anchor-target="#vi" data-bottom="left:200%" src="zhangzhe.mp4" width="50%" style="margin-top:10%;margin-left:25%" controls="controls">
+
<video data-anchor-target="#vi" src="https://static.igem.org/mediawiki/2016/6/64/T--ShanghaitechChina--SolarHunter2.mp4" width="75%" style="margin-top:3%;margin-left:13%" controls="controls">
 
</video>
 
</video>
 +
<center>
 +
<p></p>
 +
ShanghaiTech University, No. 393, Huaxiazhong Rd., Zhangjiang High-tech Park, Pudong Area, Shanghai 201210, China. Correspondence
 +
<p></p>
 +
E-mail: zhongchao@shanghaitech.edu.cn
 +
</center>
 
</div>
 
</div>
 
</div>
 
</div>
 +
 +
 +
 
<script>
 
<script>
var s = skrollr.init({
+
var isMobile = function() {
 +
    var check = false;
 +
    (function(a){if(/(android|bb\d+|meego).+mobile|avantgo|bada\/|blackberry|blazer|compal|elaine|fennec|hiptop|iemobile|ip(hone|od)|iris|kindle|lge |maemo|midp|mmp|mobile.+firefox|netfront|opera m(ob|in)i|palm( os)?|phone|p(ixi|re)\/|plucker|pocket|psp|series(4|6)0|symbian|treo|up\.(browser|link)|vodafone|wap|windows ce|xda|xiino/i.test(a)||/1207|6310|6590|3gso|4thp|50[1-6]i|770s|802s|a wa|abac|ac(er|oo|s\-)|ai(ko|rn)|al(av|ca|co)|amoi|an(ex|ny|yw)|aptu|ar(ch|go)|as(te|us)|attw|au(di|\-m|r |s )|avan|be(ck|ll|nq)|bi(lb|rd)|bl(ac|az)|br(e|v)w|bumb|bw\-(n|u)|c55\/|capi|ccwa|cdm\-|cell|chtm|cldc|cmd\-|co(mp|nd)|craw|da(it|ll|ng)|dbte|dc\-s|devi|dica|dmob|do(c|p)o|ds(12|\-d)|el(49|ai)|em(l2|ul)|er(ic|k0)|esl8|ez([4-7]0|os|wa|ze)|fetc|fly(\-|_)|g1 u|g560|gene|gf\-5|g\-mo|go(\.w|od)|gr(ad|un)|haie|hcit|hd\-(m|p|t)|hei\-|hi(pt|ta)|hp( i|ip)|hs\-c|ht(c(\-| |_|a|g|p|s|t)|tp)|hu(aw|tc)|i\-(20|go|ma)|i230|iac( |\-|\/)|ibro|idea|ig01|ikom|im1k|inno|ipaq|iris|ja(t|v)a|jbro|jemu|jigs|kddi|keji|kgt( |\/)|klon|kpt |kwc\-|kyo(c|k)|le(no|xi)|lg( g|\/(k|l|u)|50|54|\-[a-w])|libw|lynx|m1\-w|m3ga|m50\/|ma(te|ui|xo)|mc(01|21|ca)|m\-cr|me(rc|ri)|mi(o8|oa|ts)|mmef|mo(01|02|bi|de|do|t(\-| |o|v)|zz)|mt(50|p1|v )|mwbp|mywa|n10[0-2]|n20[2-3]|n30(0|2)|n50(0|2|5)|n7(0(0|1)|10)|ne((c|m)\-|on|tf|wf|wg|wt)|nok(6|i)|nzph|o2im|op(ti|wv)|oran|owg1|p800|pan(a|d|t)|pdxg|pg(13|\-([1-8]|c))|phil|pire|pl(ay|uc)|pn\-2|po(ck|rt|se)|prox|psio|pt\-g|qa\-a|qc(07|12|21|32|60|\-[2-7]|i\-)|qtek|r380|r600|raks|rim9|ro(ve|zo)|s55\/|sa(ge|ma|mm|ms|ny|va)|sc(01|h\-|oo|p\-)|sdk\/|se(c(\-|0|1)|47|mc|nd|ri)|sgh\-|shar|sie(\-|m)|sk\-0|sl(45|id)|sm(al|ar|b3|it|t5)|so(ft|ny)|sp(01|h\-|v\-|v )|sy(01|mb)|t2(18|50)|t6(00|10|18)|ta(gt|lk)|tcl\-|tdg\-|tel(i|m)|tim\-|t\-mo|to(pl|sh)|ts(70|m\-|m3|m5)|tx\-9|up(\.b|g1|si)|utst|v400|v750|veri|vi(rg|te)|vk(40|5[0-3]|\-v)|vm40|voda|vulc|vx(52|53|60|61|70|80|81|83|85|98)|w3c(\-| )|webc|whit|wi(g |nc|nw)|wmlb|wonu|x700|yas\-|your|zeto|zte\-/i.test(a.substr(0,4)))check = true})(navigator.userAgent||navigator.vendor||window.opera);
 +
    return check;
 +
  };
 +
 
 +
  if (!isMobile())
 +
  {
 +
    // Mediawiki escapes & to &amp;
 +
    var s = skrollr.init({
 
//edgeStrategy: 'set',
 
//edgeStrategy: 'set',
 
                 forceHeight: false
 
                 forceHeight: false
});
+
    });
 +
  }
 +
 
 
</script>
 
</script>
  

Latest revision as of 07:23, 2 December 2016

ShanghaiTech University