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           Design System Overview :
 
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           The core of our new diagnostic device consists of two and gate  
 
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             Take a look the details of our Stochastic and  
 
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               Deterministic modular model.
 
          
 
          
 
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             Part Overview :
 
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             The core of our new diagnostic device consists of two and gte and one irreversible switch  
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             The core of our new diagnostic device consists of two and gates and one irreversible switch  
 
        
 
        
 
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        A DIAGNOSTIC TOOL FOR <i>IN VIVO</i> SCREENING OF BIOMARKERS
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                <h2>ABSTRACT</h2>
 
                 <p>Inflammatory bowel disease (IBD) results in chronic inflammation of the intestines. Current diagnostic methods are invasive and rely on biomarkers that are not sufficiently disease-specific. We have engineered <i>E. coli</i> to detect several disease-specific biomarkers, memorize this event, and allow specific readout of the memory state. While the sensor cells travel through the gut, simultaneously occurring signals are memorized by activating an AND gate which triggers a recombination-based unidirectional switch and commits the observation to memory. After isolation from the patient’s faeces, the memory can be read out through the expression of a fluorescent protein induced by the addition of the candidate biomarker. Thus a single fluorescent protein can differentiate between many different candidate markers. A community of sensor cells can be utilized at the same time, enabling a high degree of multiplexing. Pavlov’s Coli is a non-invasive diagnostic tool for a large selection of specific biomarkers associated with IBD.
 
                 <p>Inflammatory bowel disease (IBD) results in chronic inflammation of the intestines. Current diagnostic methods are invasive and rely on biomarkers that are not sufficiently disease-specific. We have engineered <i>E. coli</i> to detect several disease-specific biomarkers, memorize this event, and allow specific readout of the memory state. While the sensor cells travel through the gut, simultaneously occurring signals are memorized by activating an AND gate which triggers a recombination-based unidirectional switch and commits the observation to memory. After isolation from the patient’s faeces, the memory can be read out through the expression of a fluorescent protein induced by the addition of the candidate biomarker. Thus a single fluorescent protein can differentiate between many different candidate markers. A community of sensor cells can be utilized at the same time, enabling a high degree of multiplexing. Pavlov’s Coli is a non-invasive diagnostic tool for a large selection of specific biomarkers associated with IBD.
 
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                 <p>The inflammatory bowel disease describes the chronic inflammations of parts of the intestine and is a collective of several further specified illnesses. The most common conditions are ulcerative colitis and Crohn's disease. It is classified as an autoimmune disease for which no cure has been developed so far. Current treatments include immunosuppression, surgery, antibiotics and nutritional therapies.<br>
 
                 <p>The inflammatory bowel disease describes the chronic inflammations of parts of the intestine and is a collective of several further specified illnesses. The most common conditions are ulcerative colitis and Crohn's disease. It is classified as an autoimmune disease for which no cure has been developed so far. Current treatments include immunosuppression, surgery, antibiotics and nutritional therapies.<br>
 
                 Unfortunately there aren't characteristic blood markers to distinguish the different forms of IBD. The diagnosis relies mostly on the location of inflammation observed during colonoscopy. Also the underlying trigger of the disease is not completely understood but correlation studies proposed factors such as diet, genetic predisposition, breach of the intestinal barrier and the composition of the microbiota, called dysbiosis. It is reported that the diversity of the microbiota is noticeably reduced in IBD patients and that the composition of the gut flora changes from symbiotic to predominantly pathobiotic microbes.</p>
 
                 Unfortunately there aren't characteristic blood markers to distinguish the different forms of IBD. The diagnosis relies mostly on the location of inflammation observed during colonoscopy. Also the underlying trigger of the disease is not completely understood but correlation studies proposed factors such as diet, genetic predisposition, breach of the intestinal barrier and the composition of the microbiota, called dysbiosis. It is reported that the diversity of the microbiota is noticeably reduced in IBD patients and that the composition of the gut flora changes from symbiotic to predominantly pathobiotic microbes.</p>
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                     <li>Email: <a href="mailto:igem2016@hiddendomain" onclick="this.href=this.href.replace(/hiddendomain/,'bsse.ethz.ch')">igem2016</a>
 
                     <li>Email: <a href="mailto:igem2016@hiddendomain" onclick="this.href=this.href.replace(/hiddendomain/,'bsse.ethz.ch')">igem2016</a>
 
                     <li>Facebook: <a href="https://www.facebook.com/iGEM.ETH.Zurich/">/iGEM.ETH.Zurich</a>
 
                     <li>Facebook: <a href="https://www.facebook.com/iGEM.ETH.Zurich/">/iGEM.ETH.Zurich</a>
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                     <li><a name="cit1" class></a>[1] Maciej Chichlowski and Laura P Hale. “Bacterial-mucosal interactions in inflammatory bowel disease: an alliance gone bad”. In: <i>American Journal of Physiology-Gastrointestinal and Liver Physiology</i> 295.6 (2008), G1139–G1149.
 
                     <li><a name="cit1" class></a>[1] Maciej Chichlowski and Laura P Hale. “Bacterial-mucosal interactions in inflammatory bowel disease: an alliance gone bad”. In: <i>American Journal of Physiology-Gastrointestinal and Liver Physiology</i> 295.6 (2008), G1139–G1149.
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Latest revision as of 01:02, 3 December 2016

PAVLOV'S COLI

A DIAGNOSTIC TOOL FOR IN VIVO SCREENING OF BIOMARKERS

ABSTRACT

Inflammatory bowel disease (IBD) results in chronic inflammation of the intestines. Current diagnostic methods are invasive and rely on biomarkers that are not sufficiently disease-specific. We have engineered E. coli to detect several disease-specific biomarkers, memorize this event, and allow specific readout of the memory state. While the sensor cells travel through the gut, simultaneously occurring signals are memorized by activating an AND gate which triggers a recombination-based unidirectional switch and commits the observation to memory. After isolation from the patient’s faeces, the memory can be read out through the expression of a fluorescent protein induced by the addition of the candidate biomarker. Thus a single fluorescent protein can differentiate between many different candidate markers. A community of sensor cells can be utilized at the same time, enabling a high degree of multiplexing. Pavlov’s Coli is a non-invasive diagnostic tool for a large selection of specific biomarkers associated with IBD.

CONTACT

Thanks to the sponsors that supported our project: