Difference between revisions of "Team:Stony Brook/Experiments"

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<h1> Protocols & Experiments </h1>
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<p>Describe the experiments, research and protocols you used in your iGEM project.</p>
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<h2> Universal Lab Protocols<h2>
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<h5>What should this page contain?</h5>
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<h3> Agarose Gel Preparation </h3>
 
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<li> Protocols </li>
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<li> Add 50mL of 1X TAE buffer to a 500mL erlenmeyer flask </li>
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<li> Experiments </li>
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<li> Add 0.5g of 1% agarose </li>
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<li>Documentation of the development of your project </li>
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<li> Microwave for three 20 second intervals and cool slightly </li>
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<li> Add 1ul Ethidium Bromide </li>
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<li> Mix and pour into gel box. Add Comb <li>
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<li> Cool for 30 minutes </li>
 
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<h5>Inspiration</h5>
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<h3> YPD Media </h3>
 
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<li><a href="https://2014.igem.org/Team:Colombia/Protocols">2014 Colombia </a></li>
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<li> <h6>For liquid media </h6></li>
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<li><a href="https://2014.igem.org/Team:Imperial/Protocols">2014 Imperial </a></li>
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<li><a href="https://2014.igem.org/Team:Caltech/Project/Experiments">2014 Caltech </a></li>
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<li> Add 20g of Bacto peptone, 10g of yeast extract and 950mL of water to a flask </li>
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<li> Autoclave on the liquid setting <li>
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<li> Add 50mL of 40% glucose and mix </li>
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</ul>
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<li><h6> For solid media</h6>
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<ul>
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<li> Add same reagents as liquid, plus 24g of Bacto agar </li>
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<li> Autoclave </li>
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<li>Stir on a magnetic stir plate and add 50ml of 40% glucose</li>
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<li> Pour into petri dishes </li>
 
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<div class="column half_size">
 
  
  
  
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<div class="column full_size">
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<h5>Inspiration</h5>
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<ul>
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<li><a href="https://2014.igem.org/Team:Colombia/Protocols">2014 Colombia </a></li>
 +
<li><a href="https://2014.igem.org/Team:Imperial/Protocols">2014 Imperial </a></li>
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<li><a href="https://2014.igem.org/Team:Caltech/Project/Experiments">2014 Caltech </a></li>
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</ul>
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Revision as of 18:40, 21 July 2016

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Protocols & Experiments

Universal Lab Protocols

Agarose Gel Preparation

  • Add 50mL of 1X TAE buffer to a 500mL erlenmeyer flask
  • Add 0.5g of 1% agarose
  • Microwave for three 20 second intervals and cool slightly
  • Add 1ul Ethidium Bromide
  • Mix and pour into gel box. Add Comb
  • Cool for 30 minutes

YPD Media

  • For liquid media
    • Add 20g of Bacto peptone, 10g of yeast extract and 950mL of water to a flask
    • Autoclave on the liquid setting
    • Add 50mL of 40% glucose and mix
  • For solid media
    • Add same reagents as liquid, plus 24g of Bacto agar
    • Autoclave
    • Stir on a magnetic stir plate and add 50ml of 40% glucose
    • Pour into petri dishes
  • Inspiration