Difference between revisions of "Team:Stony Brook/Experiments"
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| + | <h3> NEB Monarch Nucleic Acid Purification Protocols </h3> | ||
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| + | <p> The following NEB kits were used and protocols can be found on their <a href="https://www.neb.com/monarch/monarch-nucleic-acid-purification-kits"> website </a></p> | ||
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| + | <li> Monarch Plasmid Miniprep Kit </li> | ||
| + | <li> Monarch DNA Gel Extraction Kit </li> | ||
| + | <li> Monarch PCR & DNA Cleanup Kit </li> | ||
| + | </ul> | ||
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Revision as of 22:01, 21 July 2016
Protocols & Experiments
Universal Lab Protocols
Agarose Gel Preparation
- Add 50mL of 1X TAE buffer to a 500mL erlenmeyer flask
- Add 0.5g of 1% agarose
- Microwave for three 20 second intervals and cool slightly
- Add 1ul Ethidium Bromide
- Mix and pour into gel box. Add Comb
- Cool for 30 minutes
YPD Media
-
For liquid media
- Add 20g of Bacto peptone, 10g of yeast extract and 950mL of water to a flask
- Autoclave on the liquid setting
- Add 50mL of 40% glucose and mix
For solid media
- Add same reagents as liquid, plus 24g of Bacto agar
- Autoclave
- Stir on a magnetic stir plate and add 50ml of 40% glucose
- Pour into petri dishes
LB Broth
Add items to flask:
- 5g Tryptone
- 2.5g Yeast Extract
- 5g NaCl
- H2O filled to 500mL
- Mix with magnetic stir bar on low heat, autoclave for liquids
Nanodrop
- Select Nucleic Acid
- Clean, drop 1ul water, measure
- Blank
- Measure with 1ul of substance
- For PCRs: 260/280 ratio should be near 2
LB Agar
Add items to flask:
- 5g Tryptone
- 2.5g Yeast Extract
- 5g NaCl
- 7.5g Agar
- H2O filled to 500mL
- Mix with magnetic stir bar on low heat, autoclave for liquids
- Monarch Plasmid Miniprep Kit
- Monarch DNA Gel Extraction Kit
- Monarch PCR & DNA Cleanup Kit
NEB Monarch Nucleic Acid Purification Protocols
The following NEB kits were used and protocols can be found on their website
Inspiration
