Difference between revisions of "Team:Stony Brook/Notebook/Cancer-W6"

(Created page with "{{Stony_Brook}} <html> <head> <title> Week 4: 8/1-8/5 </title> </head> <body> <div class="column full_size"> <ul> <hr> </ul> </div> <div class="column_full size"> <div align...")
 
Line 17: Line 17:
 
</div>
 
</div>
 
</div>
 
</div>
 +
 +
<h6> Emmanuel Ligation (193 ng/ul) </h6>
 +
<ul>
 +
<table>
 +
<tr>
 +
<th> Content </th>
 +
<th> Experimental (ul) </th>
 +
<th> Control (ul) </th>
 +
</tr>
 +
<tr>
 +
<td> H<sub>2</sub>O</td>
 +
<td> 38.8 </td>
 +
<td> 39.8 </td>
 +
</tr>
 +
<tr>
 +
<td> Cutsmart </td>
 +
<td> 5.0 </td>
 +
<td> 5.0 </td>
 +
</tr>
 +
<tr>
 +
<td> XbaI </td>
 +
<td> 0.5 </td>
 +
<td> X </td>
 +
</tr>
 +
<tr>
 +
<td> EcoRI-HF </td>
 +
<td> 0.5 </td>
 +
<td> X </td>
 +
</tr>
 +
<tr>
 +
<td> DNA </td>
 +
<td> 5.18 </td>
 +
<td> 5.18 </td>
 +
</tr>
 +
</table>
 +
</ul>
 +
 +
<br>
 +
 +
<h6> Gel run </h6>
 +
<p> Lanes: </p>
 +
<ol>
 +
<li> Ladder </li>
 +
<li> Emmanuel Digest Control </li>
 +
<li> Emmanuel Digest Experimental </li>
 +
</ol>
 +
<ul>
 +
<li> Did not work </li>
 +
</ul>
 +
 +
<br>
 +
 +
<h6> 25 ul Digest of Ryan + Emmanuel (Veronica) Miniprep </h6>
 +
<ul>
 +
<li> Made 17 LB With Ampicillin (600ml)</li>
 +
</ul>
 +
</div>
 +
 +
<div class="column full_size">
 +
<div align="center">
 +
<h5> Ryan - 446.4 ng/ul </h5>
 +
</div>
 +
<ul>
 +
</table>
 +
<tr>
 +
<th> Content </th>
 +
<th> Experimental (ul) </th>
 +
<th> Control (ul) </th>
 +
</tr>
 +
<tr>
 +
<td> H<sub>2</sub>O</td>
 +
<td> 20.4 </td>
 +
<td> 21.4 </td>
 +
</tr>
 +
<tr>
 +
<td> Cutsmart </td>
 +
<td> 2.5 </td>
 +
<td> 2.5 </td>
 +
</tr>
 +
<tr>
 +
<td> XbaI </td>
 +
<td> 0.5 </td>
 +
<td> X </td>
 +
</tr>
 +
<tr>
 +
<td> EcoRI-HF </td>
 +
<td> 0.5 </td>
 +
<td> X </td>
 +
</tr>
 +
<tr>
 +
<td> DNA </td>
 +
<td> 1.12</td>
 +
<td> 1.12 </td>
 +
</tr>
 +
</table>
 +
</ul>
 +
</div>
 +
 +
<div class="column full_size">
 +
<div align="center">
 +
<h5> Emmanuel - 193 ng/ul </h5>
 +
</div>
 +
<ul>
 +
</table>
 +
<tr>
 +
<th> Content </th>
 +
<th> Experimental (ul) </th>
 +
<th> Control (ul) </th>
 +
</tr>
 +
<tr>
 +
<td> H<sub>2</sub>O</td>
 +
<td> 18.9 </td>
 +
<td> 19.9 </td>
 +
</tr>
 +
<tr>
 +
<td> Cutsmart </td>
 +
<td> 2.5 </td>
 +
<td> 2.5 </td>
 +
</tr>
 +
<tr>
 +
<td> XbaI </td>
 +
<td> 0.5 </td>
 +
<td> X </td>
 +
</tr>
 +
<tr>
 +
<td> EcoRI-HF </td>
 +
<td> 0.5 </td>
 +
<td> X </td>
 +
</tr>
 +
<tr>
 +
<td> DNA </td>
 +
<td> 2.59</td>
 +
<td> 2.59 </td>
 +
</tr>
 +
</table>
 +
</ul>
 +
</div>
 +
 +
<div class="column full_size">
 +
<div align="center">
 +
<h2> 8/2 </h2>
 +
</div>
 +
 +
<h6> Miniprep </h6>
 +
<ul>
 +
<li> Miniprep of ligated construct </li>
 +
<li> 10,000rpm for 3 minutes at 25°C </li>
 +
</ul>
 +
 +
<h6> Ran a nanodrop of Minipreps </h6>
 +
<ul>
 +
<table>
 +
<tr>
 +
<th> Content </th>
 +
<th> Concentration (ng/ul) </th>
 +
<th> 260/280 </th>
 +
</tr>
 +
<tr>
 +
<td> Veronica 1 </td>
 +
<td> 311.5 </td>
 +
<td> 1.86 </td>
 +
</tr>
 +
<tr>
 +
<td> Veronica 2 </td>
 +
<td> 43.8 </td>
 +
<td> 1.76 </td>
 +
</tr>
 +
<tr>
 +
<td> Veronica 3</td>
 +
<td> 259 </td>
 +
<td> 1.88 </td>
 +
</tr>
 +
<tr>
 +
<td> Veronica 4 </td>
 +
<td> 296 </td>
 +
<td> 1.88 </td>
 +
</tr>
 +
<tr>
 +
<td> R1 </td>
 +
<td> 176.5 </td>
 +
<td> 1.75 </td>
 +
</tr>
 +
<tr>
 +
<td> Ryan 2 </td>
 +
<td> 277.3 </td>
 +
<td> 1.85 </td>
 +
</tr>
 +
<tr>
 +
<td> Ryan 3 </td>
 +
<td> 301.6 </td>
 +
<td> 1.81 </td>
 +
</tr>
 +
<tr>
 +
<td> Ryan 4 </td>
 +
<td> 366.6 </td>
 +
<td> 1.82 </td>
 +
</tr>
 +
</div>
 +
</ul>
 +
 +
<h6> Ran a gel of miniprepped plasmids </h6>
 +
<ul>
 +
<li> Used 5ul of DNA and 1 ul of dye </li>
 +
</ul>
 +
<p> Lane: </p>
 +
<ol>
 +
<li> Veronica 1 </li>
 +
<li> Veronica 3 </li>
 +
<li> Veronica 4 </li>
 +
<li> Ladder </li>
 +
<li> Ryan 1 </li>
 +
<li> Ryan 2 </li>
 +
<li> Ryan 3 </li>
 +
<li> Ryan 4 </li>
 +
</ol>

Revision as of 22:39, 2 August 2016

MENU ▤

Week 4: 8/1-8/5

8/1

Emmanuel Ligation (193 ng/ul)
    Content Experimental (ul) Control (ul)
    H2O 38.8 39.8
    Cutsmart 5.0 5.0
    XbaI 0.5 X
    EcoRI-HF 0.5 X
    DNA 5.18 5.18

Gel run

Lanes:

  1. Ladder
  2. Emmanuel Digest Control
  3. Emmanuel Digest Experimental
  • Did not work

25 ul Digest of Ryan + Emmanuel (Veronica) Miniprep
  • Made 17 LB With Ampicillin (600ml)
Ryan - 446.4 ng/ul
    Content Experimental (ul) Control (ul) H2O 20.4 21.4 Cutsmart 2.5 2.5 XbaI 0.5 X EcoRI-HF 0.5 X DNA 1.12 1.12
Emmanuel - 193 ng/ul
    Content Experimental (ul) Control (ul) H2O 18.9 19.9 Cutsmart 2.5 2.5 XbaI 0.5 X EcoRI-HF 0.5 X DNA 2.59 2.59

8/2

Miniprep
  • Miniprep of ligated construct
  • 10,000rpm for 3 minutes at 25°C
Ran a nanodrop of Minipreps
    Ran a gel of miniprepped plasmids
    • Used 5ul of DNA and 1 ul of dye

    Lane:

    1. Veronica 1
    2. Veronica 3
    3. Veronica 4
    4. Ladder
    5. Ryan 1
    6. Ryan 2
    7. Ryan 3
    8. Ryan 4

    Content Concentration (ng/ul) 260/280
    Veronica 1 311.5 1.86
    Veronica 2 43.8 1.76
    Veronica 3 259 1.88
    Veronica 4 296 1.88
    R1 176.5 1.75
    Ryan 2 277.3 1.85
    Ryan 3 301.6 1.81
    Ryan 4 366.6 1.82