Difference between revisions of "Team:Stony Brook/Notebook/Cancer-W6"
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<h5> Emmanuel - 193 ng/ul </h5> | <h5> Emmanuel - 193 ng/ul </h5> | ||
Revision as of 22:44, 2 August 2016
8/1
Emmanuel Ligation (193 ng/ul)
| Content | Experimental (ul) | Control (ul) |
|---|---|---|
| H2O | 38.8 | 39.8 |
| Cutsmart | 5.0 | 5.0 |
| XbaI | 0.5 | X |
| EcoRI-HF | 0.5 | X |
| DNA | 5.18 | 5.18 |
Gel run
Lanes:
- Ladder
- Emmanuel Digest Control
- Emmanuel Digest Experimental
- Did not work
25 ul Digest of Ryan + Emmanuel (Veronica) Miniprep
- Made 17 LB With Ampicillin (600ml)
Ryan - 446.4 ng/ul
| Content | Experimental (ul) | Control (ul) |
|---|---|---|
| H2O | 20.4 | 21.4 |
| Cutsmart | 2.5 | 2.5 |
| XbaI | 0.5 | X |
| EcoRI-HF | 0.5 | X |
| DNA | 1.12 | 1.12 |
Emmanuel - 193 ng/ul
| Content | Experimental (ul) | Control (ul) |
|---|---|---|
| H2O | 18.9 | 19.9 |
| Cutsmart | 2.5 | 2.5 |
| XbaI | 0.5 | X |
| EcoRI-HF | 0.5 | X |
| DNA | 2.59 | 2.59 |
8/2
Miniprep
- Miniprep of ligated construct
- 10,000rpm for 3 minutes at 25°C
Ran a nanodrop of Minipreps
| Content | Concentration (ng/ul) | 260/280 |
|---|---|---|
| Veronica 1 | 311.5 | 1.86 |
| Veronica 2 | 43.8 | 1.76 |
| Veronica 3 | 259 | 1.88 |
| Veronica 4 | 296 | 1.88 |
| R1 | 176.5 | 1.75 |
| Ryan 2 | 277.3 | 1.85 |
| Ryan 3 | 301.6 | 1.81 |
| Ryan 4 | 366.6 | 1.82 |
Ran a gel of miniprepped plasmids
- Used 5ul of DNA and 1 ul of dye
Lane:
- Veronica 1
- Veronica 3
- Veronica 4
- Ladder
- Ryan 1
- Ryan 2
- Ryan 3
- Ryan 4
