Difference between revisions of "Team:Stony Brook/Notebook/Cancer-W7"

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</table>
 
</table>
 
</ul>
 
</ul>
−
</div>
+
 
 +
<h4> PCR Settings Template: </h4>
 +
 
 +
<ul>
 +
<table>
 +
<tr>
 +
<th> Phase </th>
 +
<th> Temperature (°C) </th>
 +
<th> Time (sec)</th>
 +
</tr>
 +
<tr>
 +
<td> Initial Denaturation </td>
 +
<td> 98 </td>
 +
<td> 60 </td>
 +
</tr>
 +
<tr>
 +
<td> Denaturation </td>
 +
<td> 98 </td>
 +
<td> 25 </td>
 +
</tr>
 +
<tr>
 +
<td> Annealing </td>
 +
<td> 66 </td>
 +
<td> 20 </td>
 +
</tr>
 +
<tr>
 +
<td> Extension </td>
 +
<td> 72 </td>
 +
<td> 45 </td>
 +
</tr>
 +
<tr>
 +
<td> Final Extension </td>
 +
<td> 72 </td>
 +
<td> 60 </td>
 +
</tr>
 +
</table>
 +
</ul>
 +
 
 +
<h4> Nanodrop Template: </h4>
 +
<ul>
 +
<table>
 +
<tr>
 +
<th> Content </th>
 +
<th> Concentration (ng/ul) </th>
 +
<th> 260/280 </th>
 +
</tr>
 +
<tr>
 +
<td> Construct </td>
 +
<td> 14.6 </td>
 +
<td> 1.85 </td>
 +
</tr>
 +
<tr>
 +
<td> Vector </td>
 +
<td> 14.6 </td>
 +
<td> 1.80 </td>
 +
</tr>
 +
<tr>
 +
</table>
 +
</ul>
 +
 
 +
<h4> Ligation Template </h4>
 +
<ul>
 +
<table>
 +
<tr>
 +
<th> Content </th>
 +
<th> ul </th>
 +
</tr>
 +
<tr>
 +
<td> 10X T4 Buffer </td>
 +
<td> 2 </td>
 +
</tr>
 +
<tr>
 +
<td> Vector </td>
 +
<td> 4 (58.4 ng) </td>
 +
</tr>
 +
<td> Construct </td>
 +
<td> 1.55 (22.69 ng) </td>
 +
</tr>
 +
<tr>
 +
<td> H<sub>2</sub>O</td>
 +
<td> 11.45 </td>
 +
</tr>
 +
<tr>
 +
<td> Ligase </td>
 +
<td> 1</td>
 +
</tr>
 +
</table>
 +
</ul>
 +
 
  
 
<div class="column full_size">
 
<div class="column full_size">

Revision as of 07:27, 5 August 2016

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Week 7: 8/8 - 8/14

Week 7: 8/8 - 8/14

8/8

PCR Table Template:
    Contents ul
    Phire 25
    H2O 23.2
    50 uM F Primer 0.5
    50 uM R Primer 0.5
    95.1ug/ul Construct 0.8

PCR Settings Template:

    Phase Temperature (°C) Time (sec)
    Initial Denaturation 98 60
    Denaturation 98 25
    Annealing 66 20
    Extension 72 45
    Final Extension 72 60

Nanodrop Template:

    Content Concentration (ng/ul) 260/280
    Construct 14.6 1.85
    Vector 14.6 1.80

Ligation Template

    Content ul
    10X T4 Buffer 2
    Vector 4 (58.4 ng)
    Construct 1.55 (22.69 ng)
    H2O 11.45
    Ligase 1

8/9

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8/11

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8/13

8/14