Difference between revisions of "Team:Stony Brook/Notebook/Cancer-W7"
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Revision as of 14:23, 9 August 2016
I left you some of the commonly used templates down there under 8/8. Just fill them in as needed. I'll only really have wifi for a few hours on two days between Saturday and Thursday. If you need anything, message me on Facebook and I'll get back to you as soon as I can.
With the warmest love and regards,
~JOSHUA
Week 7: 8/8 - 8/14
PCR Table Template:
| Contents | ul | Phire | # |
|---|---|
| H2O | # |
| 50 uM F Primer | # |
| 50 uM R Primer | # |
| #ug/ul Construct | # |
PCR Settings Template:
| Phase | Temperature (°C) | Time (sec) |
|---|---|---|
| Initial Denaturation | 98 | # |
| Denaturation | 98 | # |
| Annealing | # | # |
| Extension | 72 | # |
| Final Extension | 72 | # |
Nanodrop Template:
| Content | Concentration (ng/ul) | 260/280 |
|---|---|---|
| Construct | # | # |
| Vector | # | # |
Ligation Template
| Content | ul |
|---|---|
| 10X T4 Buffer | # |
| Vector | # (# ng) | Construct | # (# ng) |
| H2O | # |
| Ligase | # |
Multiple Digests Template:
This
| Content | Experimental ul | Control ul |
|---|---|---|
| H2O | # | # |
| Cutsmart | # | # |
| EcoRI-HF | # | X |
| XbaI | # | X |
| DNA | # | # |
Is
| Content | Experimental ul | Control ul |
|---|---|---|
| H2O | # | # |
| Cutsmart | # | # |
| EcoRI-HF | # | X |
| XbaI | # | X |
| DNA | # | # |
A
| Content | Experimental ul | Control ul |
|---|---|---|
| H2O | # | # |
| Cutsmart | # | # |
| EcoRI-HF | # | X |
| XbaI | # | X |
| DNA | # | # |
Thing
| Content | Experimental ul | Control ul |
|---|---|---|
| H2O | # | # |
| Cutsmart | # | # |
| EcoRI-HF | # | X |
| XbaI | # | X |
| DNA | # | # |
8/8
Sequencing
| T1 | T2 |
|---|---|
| 5.34 uL H2O | 5.34 uL H2O |
| 1 uL CR-1 Primer 1 | 1 uL CR-1 Primer 2 | 1.66 uL DNA (301.6 ng/uL) | 1.66 uL DNA (301.6 ng/uL) |
Diluted BioBrick MiniPreps for PCR
| BioBrick Name | Concentration (ng/uL) | Contents (DNA + water) |
|---|---|---|
| K592009 | 76.9 | 1 uL + 6.69 uL water |
| B0015 | 51.7 | 1 uL + 4.17 uL water |
| K1033910 | 156.5 | 1 uL + 14.65 uL water |
| R0082 | 49.5 | 1 uL + 3.95 uL water |
| C0012 | 150.8 | 1 uL + 14.08 uL water |
| J61100 | 123.0 | 1 uL + 11.3 uL water |
| R0011 | 56.7 | 1 uL + 4.67 uL water |
PCR of each BioBrick parts (25 uL Q5 MasterMix rxns)
| Contents | uL |
|---|---|
| 10 uM VF2 primer | 1.25 uL |
| 10 uM VR primer | 1.25 uL |
| DNA | 0.5 uL |
| Q5 MM | 12.5 uL |
| Water | 19.5 uL |
Gel ran
Lanes:
- Ladder
- R0011
- B001
- K1033
- R008
- J61100
- C0012
- K592
8/9
8/10
8/11
8/12
8/13
8/14
