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<h1> Week 9: 8/22 - 8/28</h1>
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<hr>
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</div>
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</div>
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<div align="center">
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<br>
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<hr>
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<br>
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</div>
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<div class="column full_size">
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<h2 >8/22 </h2>
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</div>
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</div>
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<div class="column full_size">
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<div align="center">
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<h4> PCR of new IDT Shipments </h4>
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</div>
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</div>
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<div class="column half_size">
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<div align="center">
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<h5> AmilCP </h5>
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<ul>
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<table>
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<tr>
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<th> Contents </th>
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<th> ul </th>
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</tr>
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<td> Q5 MasterMix </td>
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<td> 12.5 </td>
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</tr>
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<tr>
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<td> H<sub>2</sub>O</td>
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<td> 9 </td>
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</tr>
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<tr>
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<td> 10 uM F Primer </td>
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<td> 1.25 </td>
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</tr>
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<tr>
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<td> 10 uM R Primer </td>
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<td> 1.25 </td>
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</tr>
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<tr>
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<td> Template </td>
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<td> 1 </td>
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</tr>
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</table>
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</ul>
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</div>
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</div>
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<div class="column half_size">
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<div align="center">
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<h5> IDT #1</h5>
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<ul>
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<table>
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<tr>
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<th> Contents </th>
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<th> ul </th>
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</tr>
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<td> Q5 MasterMix </td>
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<td> 12.5 </td>
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</tr>
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<tr>
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<td> H<sub>2</sub>O</td>
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<td> 6.5</td>
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</tr>
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<tr>
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<td> 10 uM F Primer </td>
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<td> 2.5 </td>
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</tr>
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<tr>
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<td> 10 uM R Primer </td>
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<td> 2.5 </td>
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</tr>
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<tr>
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<td> Template </td>
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<td> 1 </td>
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</tr>
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</table>
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</ul>
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</div>
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</div>
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<div class="column full_size">
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<br>
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</div>
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<div class="column half_size">
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<div align="center">
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<h5> IDT #2</h5>
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<ul>
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<table>
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<tr>
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<th> Contents </th>
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<th> ul </th>
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</tr>
 +
<td> Q5 MasterMix </td>
 +
<td> 12.5 </td>
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</tr>
 +
<tr>
 +
<td> H<sub>2</sub>O</td>
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<td> 6.5</td>
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</tr>
 +
<tr>
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<td> 10 uM F Primer </td>
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<td> 2.5 </td>
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</tr>
 +
<tr>
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<td> 10 uM R Primer </td>
 +
<td> 2.5 </td>
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</tr>
 +
<tr>
 +
<td> Template </td>
 +
<td> 1 </td>
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</tr>
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</table>
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</ul>
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</div>
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</div>
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<div class="column half_size">
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<div align="center">
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<h4> PCR Settings: 25 Cycles
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<ul>
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<table>
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<tr>
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<th> Phase </th>
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<th> Temperature (°C) </th>
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<th> Time (sec)</th>
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</tr>
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<tr>
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<td> Initial Denaturation </td>
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<td> 98 </td>
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<td> 30</td>
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</tr>
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<tr>
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<td> Denaturation </td>
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<td> 98 </td>
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<td> 10 </td>
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</tr>
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<tr>
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<td> Annealing </td>
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<td> 65</td>
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<td> 30 </td>
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</tr>
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<tr>
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<td> Extension </td>
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<td> 72 </td>
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<td> 120 </td>
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</tr>
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<tr>
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<td> Final Extension </td>
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<td> 72 </td>
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<td> 120 </td>
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</tr>
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</table>
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</ul>
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<div align="center">
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<br>
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<hr>
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<br>
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</div>
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<div class="column full_size">
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<div align="center">
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<h2> 8/23 </h2>
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</div>
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<div class="column full_size">
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<div align="center">
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</div>
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<hr>
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<br>
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</div>
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<div class="column full_size">
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<h2> 8/24 </h2>
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</body>
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Revision as of 21:11, 23 August 2016

Week 9: 8/22 - 8/28

Week 9: 8/22 - 8/28



8/22

PCR of new IDT Shipments

AmilCP
    Contents ul
    Q5 MasterMix 12.5
    H2O 9
    10 uM F Primer 1.25
    10 uM R Primer 1.25
    Template 1
IDT #1
    Contents ul
    Q5 MasterMix 12.5
    H2O 6.5
    10 uM F Primer 2.5
    10 uM R Primer 2.5
    Template 1

IDT #2
    Contents ul
    Q5 MasterMix 12.5
    H2O 6.5
    10 uM F Primer 2.5
    10 uM R Primer 2.5
    Template 1

PCR Settings: 25 Cycles
    Phase Temperature (°C) Time (sec)
    Initial Denaturation 98 30
    Denaturation 98 10
    Annealing 65 30
    Extension 72 120
    Final Extension 72 120


8/23



8/24