Difference between revisions of "Team:SUSTech Shenzhen/Hardware"
| Line 6: | Line 6: | ||
{{:Team:SUSTech_Shenzhen/main-content-begin}} | {{:Team:SUSTech_Shenzhen/main-content-begin}} | ||
| + | = Introduction = | ||
| + | We are aiming to evolve a more sensitive mechanical sensitive channel (MS channel). When MS channels are stimulated by mechanical force, a Ca2+ influx is triggered. To measure the performance of MS channel, we chose sound to produce mechanical force and GECO protein as Ca2+ indicator to reflect the channel sensitivity through its fluorescence change. | ||
| + | == Sound part == | ||
| + | |||
| + | In this part, we aim to explore the effect different frequency on cell. Frequencies from 300Hz to 1.7MHz were tried to observe whether the cell with MS channel can response differently to different frequency. If so, frequency orthogonal audiogenetic parts are hopefully to be developed. Meanwhile, the equipment was also designed to be easy to change sonic power, in order to choose the most suitable intensity being harmless to cell. | ||
| + | |||
| + | === Sonic Stimulation === | ||
| + | |||
| + | ==== Signal generation ==== | ||
| + | |||
| + | A dual channel function/arbitrary waveform generator (Feel Tech FY2303) was used to generate sound wave signals. It can generate 1uHz to 2MHz, 0v to 20v signals. An amplifier was used to amplify the wave signal output by the wave generator.[[File: SUSTech_Shenzhen-EAE28F33-1AC9-419E-80F9-ECA6CCA11AE6.png | 800px | thumb | left]] | ||
| + | |||
| + | ==== Sound generator ==== | ||
| + | |||
| + | ===== buzzer ===== | ||
| + | |||
| + | Buzzer is a thin metal plate attached with a piezoelectric ceramic. When varying voltage is added to its two ends, it deforms coordinately. Whereas, its low and high frequency response is weak, and can only be used to generate sound under 15 KHz. For cell safety, we fix it on the bottom of cell dish for cell stimulation. [[File: SUSTech_Shenzhen-02C459B7-C804-42FE-BB9A-1466379C37FB.png | 800px | thumb | left]] | ||
Revision as of 06:10, 13 October 2016
Hardware
Model
Contents
Introduction
We are aiming to evolve a more sensitive mechanical sensitive channel (MS channel). When MS channels are stimulated by mechanical force, a Ca2+ influx is triggered. To measure the performance of MS channel, we chose sound to produce mechanical force and GECO protein as Ca2+ indicator to reflect the channel sensitivity through its fluorescence change.
Sound part
In this part, we aim to explore the effect different frequency on cell. Frequencies from 300Hz to 1.7MHz were tried to observe whether the cell with MS channel can response differently to different frequency. If so, frequency orthogonal audiogenetic parts are hopefully to be developed. Meanwhile, the equipment was also designed to be easy to change sonic power, in order to choose the most suitable intensity being harmless to cell.
Sonic Stimulation
Signal generation
A dual channel function/arbitrary waveform generator (Feel Tech FY2303) was used to generate sound wave signals. It can generate 1uHz to 2MHz, 0v to 20v signals. An amplifier was used to amplify the wave signal output by the wave generator.
Sound generator
buzzer
Buzzer is a thin metal plate attached with a piezoelectric ceramic. When varying voltage is added to its two ends, it deforms coordinately. Whereas, its low and high frequency response is weak, and can only be used to generate sound under 15 KHz. For cell safety, we fix it on the bottom of cell dish for cell stimulation.
