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Revision as of 00:19, 9 October 2016
Wiki under construction
Describe the experiments, research and protocols you used in your iGEM project.
What should this page contain?
- Protocols
- Experiments
- Documentation of the development of your project
Inspiration
Component | 50 µl Reaction | |
Nuclease-free water | to 50 µl | |
5X Phusion HF | 10 µl | |
10 mM dNTPs | 1 µl | |
10 µM Forward Primer | 2.5 µl | |
10 µM Reverse Primer | 2.5 µl | |
Template DNA | variable | < 250 ng |
DMSO (optional) | (1.5 µl, up to 3%) | |
Phusion DNA Polymerase |
0.5 µl |