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<a href="https://2016.igem.org/Team:BIT-China"> | <a href="https://2016.igem.org/Team:BIT-China"> |
Revision as of 14:01, 11 October 2016
![title_img](https://static.igem.org/mediawiki/2016/c/cf/T--BIT-China--title_img.png)
![circle_img](https://static.igem.org/mediawiki/2016/b/ba/T--BIT-China--circle_logo_under.png)
![circle_img](https://static.igem.org/mediawiki/2016/9/9c/T--BIT-China--circle_logo_center.png)
![circle_img](https://static.igem.org/mediawiki/2016/a/a8/T--BIT-China--circle_logo_up.png)
![home_title_down](https://static.igem.org/mediawiki/2016/e/e5/T--BIT-China--img--home_title_down.png)
Kill the Slacker
Monitoring Plasmid Numbers
![overtop](https://static.igem.org/mediawiki/2016/1/10/T--BIT-China--over_top.png)
![overview1](https://static.igem.org/mediawiki/2016/1/14/T--BIT-China--img--home_overview1.png)
production, due to the random distribution of
plasmids in the process of bacteria proliferation
and division, the bacteria containing less
plasmids will gradually become the
“slackers”.
![overview2](https://static.igem.org/mediawiki/2016/6/60/T--BIT-China--img--home_overview2.png)
bacteria become the dominant population.
There is no efficient way to prevent the birth of
plasmid-free bacteria and the applied ways are
only limited to the laboratory-use.
![overview3](https://static.igem.org/mediawiki/2016/b/b2/T--BIT-China--img--home_overview3.png)
What about equipping the bacteria with a
plasmid-sensing logically adjustable cell killer (P-SLACKiller)?
plasmid-sensing logically adjustable cell killer (P-SLACKiller)?
Here is killer’s basic rule:
Kill the slackers (Plasmid numbers are above a
threshold);
Save the workers (Plasmid numbers are below
the threshold).
Kill the slackers (Plasmid numbers are above a
threshold);
Save the workers (Plasmid numbers are below
the threshold).
![overview4](https://static.igem.org/mediawiki/2016/8/8d/T--BIT-China--img--home_overview4.png)
Surprise! We can guarantee
the microbial
population structure stay optimal now!
population structure stay optimal now!
![overview5](https://static.igem.org/mediawiki/2016/4/47/T--BIT-China--img--home_overview5.png)
Our team divide the experiment into 4 parts as below.
Why not click and have a look?
![home-basic](https://static.igem.org/mediawiki/2016/f/f7/T--BIT-China--img--home_basic.png)
Basic Circuit
We know low-plasmid level cells cost too much but produce less,like a slacker.So let's kill them to improve production.
![home-basic](https://static.igem.org/mediawiki/2016/2/2b/T--BIT-China--img--home_Inhibitor.png)
Inhibitor
Yes,we set a BOMB in every cell,inside the plasmid.They are controlled to Terminate the cell's life.
![home-basic](https://static.igem.org/mediawiki/2016/2/2c/T--BIT-China--img--home_killer.png)
Killer
Our project depends on good models.Every step we go all with the direction of our model part.
![home-basic](https://static.igem.org/mediawiki/2016/8/81/T--BIT-China--img--home_recombination.png)
Recombination
Considering the safety requirements,our project is environment-friendly .