Team:BIT-China/E M
What is the point?
Just show what things we use in the experiments.
Equipments
| Name | Type | Company |
|---|---|---|
| Transferpettor | 1000µl 100µl 10µl 2.5µl | Eppendorf |
| ELIASA | Elx808 | America Bioteck company |
| Electrophresis Apparatus | DYY-6C | Beijing LIUYI bio-technology company |
| OD Machine | UV-2100 | UNICO |
| Incubator Shaker | IS-RDS3 | Beijing KECHUANGBAIFANG Technical Co., Ltd |
| Electro-heating Standing-temperature Water Bath | DK-8D | Shanghai YIHENG Technical Co., Ltd |
| Gel-imaging System | GeneGenius | SYNGENE |
| Blue Rubber Cutting Machine | BD-BGCI | Biofriend |
| Electro-heating Standing-temperature Cultivator | SKP-02.420 | America HENGFENG company |
| High Speed Freezing Centrifuge | D-37520 | Germany Thermo company |
| Low temperature enzyme even instrument | L1 | HEMA |
| Electronic Balance | FA2104SW | MINQIAO |
| High-Pressure Steam Sterilization Pot | MLS-3750 | SANYO |
| Laminar Flow Cabinet | SW-CJ-2FD | Taisite |
| Microwave（微波炉） | Galanz | |
| Electro-heating Standing-temperature Drum Wind Drying Machine | DHG-9030A | Shanghai YIHENG Technical Co., Ltd |
| 96 Well Thermal Cycler（PCR仪） | Gene Company Limited | |
| High Speed Micro-Centrifuge | D3024 | SCILOGEX |
| Refrigerator | BCD-610W | SIEMENS |
| Heto Ultra Freeze(-80℃ 冰箱) | Thermo | |
| Ultrapure Water System（超纯水系统） | ||
| Ice Machine | SPR-120 | SIMAG |
| Nano Drop | 2000c | America Thermo Scientific company |
| Microscope（显微镜） | Ruich Allway | |
| Noise Isolating Tamber | vcx750 | Ningbo Scientz Biotechnology CO. , Ltd |
| Noise Cleaner | KQ-100DE | Kunshang Noise Equipment CO. Ltd. |
Reagent & Material
1. antibiotic
Ampicillin: dissolved in distilled water and the final concentration is 100mg/ml
Chloromycetin : dissolved in absolute ethyl alcohol and final concentration is 25 mg/ml
Kanamycin: dissolved by distilled water and final concentration is 50 mg/ml
2. Maker
Trans 2K DNA Maker
Trans 2K plus DNA Maker
Trans 1KB DNA Maker
Trans 2K plusⅡDNA Maker
All from Trans Gen Biotech
3. Buffer
| Buffer | From |
|---|---|
| 6XDNA Loading buffer | Trans Gen Biotech |
| 2×Primer star CG buffer | TaKaRa |
| 2×Taq PCR Master Mix | Biomed |
| 2×ES Taq Master Mix | CWBio |
| 10×T4 DNA Ligase Buffer | TaKaRa |
| 10×Fast Digest Green Buffer | Scientific |
| 10×Fast Digest Buffer | Scientific |
| 10×Pfu Buffer | Biomed |
| 5×Trans Start FastPfu Buffer | Trans Gen Biotech |
| 5×FastPfu Fly Buffer | Trans Gen Biotech |
4.
10% glycerol: 12.6g glycerol dissolved in 90 ml of water.
50%glycerol: use distilled water to dilute 50ml glycerol twice as much
5. dd H2O
6. Enzyme
| Enzyme | From |
|---|---|
| T4 DNA Ligase | Thermo Scientific |
| Primer star HS DNA polymerase | TaKaRa |
| Pfu DNA polymerase | Biomed |
| Trans Start FastPfu DNA polymerase | Trans Gen Biotech |
| Trans Start FastPfu Fly DNA polymerase | Trans Gen Biotech |
| Fast Digest DpnⅠ | Thermo Scientific |
| Fast digest EcoRⅠ | Thermo Scientific |
| Fast digest PstⅠ | Thermo Scientific |
| Fast digest XbaⅠ | Thermo Scientific |
| Fast digest SpeⅠ | Thermo Scientific |
7. absolute ethyl alcohol
8. Kit
TIANgel Midi Purification Kit
TIANprep Mini plasmid Kit
TIANamp Bacteria DNA Kit
All from TIANGEN BIOTECH (BEIJING) CO.LTD
9. Agarose Gel Electrophoresis:
Golden View from Biomed
BIOWEST REGULAR AGAROSE G-10
1XTAE：Diluted with 50Xtae
50XTAE: Tris 242g，Na2EDTA·2H2O 37.2g , 57.1ml glacial acetic acid,1L water
10. Culture medium
LB: 10g Tryptone peptone,5g Yeast extract, 5gNaCl; dissolve in 1.0L water.
SOB:2.0g Tryptone peptone,0.5g Yeast extract,0.2ml 5M NaCl,0.25ml
1M KCL; dissolve in 90 ml water. Adjust PH to 7.0.Bring volume to
100 ml. Autoclave. Add 1.0 ml sterile 1 M MgCl2 and 1.0 ml sterile 1 M MgSO4.
11. Arabinose
1 M Arabinose: weight 150g then use distilled water to dilute it
10% Arabinose: use distilled water to dilute 1M Arabinose
12. IPTG: confect the final concentration of 1M IPTG with distilled water
13. High pure dNTPs from Trans Gen Biotech
13. High pure dNTPs from Trans Gen Biotech
14. Bacteria
TOP10 from Biomed
DH5α from Biomed
15. Golden View from Biomed
