Team:BIT-China/Parts
·Part collection·
In 2016, iGEM BIT-CHINA has submitted 69 parts. We divide our parts into 5 group. Each group contains some parts with similar function or close relation. Different groups also connect with each other.
Group1: killer
BBa_K2120002~BBa_K2120006 is a collection of arabinose induced toxin protein expression cassette, we test these part to evaluate the efficient of the killer.
Group2: reverse
BBa_K2120100~BBa_K2120107 is a collection of reverse components including promoters and coding protein, we construct these part to help us build a reverse device. A reverse device avoid two devices in a circuit affect each other.
Group3: mutation
BBa_K2120200~BBa_K2120207 is a collection of mutated promoters and the testing devices. We use mutated promoter to control the efficient of the killer and select an appropriate one.
Group4: inhibitor
BBa_K2120303~BBa_K2120307 is a collection of arabinose induced inhibitor expression cassette.
BBa_K2120308~BBa_K2120311 is a collection of arabinose induced final testing circuits. We use it to evaluate the efficient of our application circuit.
BBa_K2120312~BBa_K2120327 is a collection of constitutively expression cassette of inhibitors to construct our final application circuit.
Group5: Application Circuit
BBa_K2120400~BBa_K2120415 is a collection of constitutive final testing circuits, toxin protein contained. These are our application circuit to carry out P-SLAKILLER function.
BBa_K2120416~BBa_K2120425 is a collection of constitutive final testing circuits, RFP contained. We use them to evaluate if our application really work.
·Part Form·
| No. | Favorite | Name | Type | Description | Length |
|---|---|---|---|---|---|
| Killer | |||||
| 1 | BBa_K2120002 | Composite | araC+PBAD+B0032+ mazF | 1572 | |
| 2 | BBa_K2120003 | Composite | araC+PBAD+B0032+ hokD | 1392 | |
| 3 | BBa_K2120004 | Composite | araC+PBAD+B0031+ mazF | 1573 | |
| 4 | BBa_K2120005 | Composite | araC+PBAD+B0031+ hokD | 1393 | |
| 5 | BBa_K2120006 | Composite | araC+PBAD+B0034+ mazF | 1571 | |
| 6 | BBa_K2120008 | Coding | mazF | 336 | |
| Reverse | |||||
| 7 | BBa_K2120100 | Regulatory | reverse aracPBAD | 1209 | |
| 8 | BBa_K2120101 | Regulatory | reverse J23119 | 35 | |
| 9 | BBa_K2120102 | Regulatory | reverse J23116 | 35 | |
| 10 | BBa_K2120103 | Regulatory | reverse J23109 | 35 | |
| 11 | BBa_K2120104 | Regulatory | reverse J23106 | 35 | |
| 12 | BBa_K2120105 | Coding | reverse cI | 775 | |
| 13 | BBa_K2120106 | Coding | reverse tetR | 685 | |
| 14 | BBa_K2120107 | Coding | reverse lacIq | 1092 | |
| Mutation | |||||
| 15 | BBa_K2120200 | Regulatory | Mutation Ptet-1 | 54 | |
| 16 | BBa_K2120201 | Regulatory | Mutation Ptet-2 | 54 | |
| 17 | BBa_K2120202 | Regulatory | Mutation Ptet-3 | 54 | |
| 18 | BBa_K2120203 | Regulatory | Mutation Ptet-25 | 54 | |
| 19 | BBa_K2120204 | Device | Ptet-1+B0034+rfp+B0015 | 923 | |
| 20 | BBa_K2120205 | Device | Ptet-2+B0034+rfp+B0015 | 923 | |
| 21 | BBa_K2120206 | Device | Ptet-3+B0034+rfp+B0015 | 923 | |
| 22 | BBa_K2120207 | Device | Ptet-25+B0034+rfp+B0015 | 923 | |
| Inhibitor | |||||
| 23 | BBa_K2120301 | Device | PR+B0032+rfp+B0015 | 919 | |
| 24 | BBa_K2120302 | Device | araC-PBAD+B0034+rfp+B0015 | 2078 | |
| 25 | BBa_K2120303 | Device | araC-PBAD +B0034+ tetR+B0015 | 2057 | |
| 26 | BBa_K2120304 | Device | araC-PBAD+B0034+ lacIq+B0015 | 2466 | |
| 27 | BBa_K2120305 | Device | araC-PBAD+B0034+ cI+B0015 | 2147 | |
| 28 | BBa_K2120306 | Composite | B0015+cI+B0034+PBAD-araC+B0015 | 2286 | |
| 29 | BBa_K2120307 | Composite | B0015+tetR+B0034+PBAD-araC+B0015 | 2196 | |
| 30 | BBa_K2120308 | Composite | B0015+lacIq+B0034+PBAD-araC+Plac+B0034+yfp+B0015 | 3599 | |
| 31 | BBa_K2120309 | Composite | B0015+cI+B0034+PBAD-araC+PR+B0032+rfp+B0015 | 3076 | |
| 32 | BBa_K2120310 | Composite | B0015+cI+B0034+PBAD-araC+B0015+PR+B0032+rfp+B0015 | 3213 | |
| 33 | BBa_K2120311 | Composite | B0015+tetR +B0034+PBAD-araC +B0015+Ptet+B0034+rfp+B0015 | 3127 | |
| 34 | BBa_K2120312 | Device | J23119+B0034+tetR+B0015 | 883 | |
| 35 | BBa_K2120313 | Device | J23119+B0034+cI+B0015 | 973 | |
| 36 | BBa_K2120314 | Device | J23119+B0032+tetR+B0015 | 884 | |
| 37 | BBa_K2120315 | Device | J23119+B0032+cI+B0015 | 974 | |
| 38 | BBa_K2120317 | Device | J23109+B0034+cI+B0015 | 973 | |
| 39 | BBa_K2120318 | Device | J23109+B0032+tetR+B0015 | 884 | |
| 40 | BBa_K2120319 | Device | J23109+B0032+cI+B0015 | 974 | |
| 41 | BBa_K2120321 | Device | J23116+B0034+cI+B0015 | 973 | |
| 42 | BBa_K2120323 | Device | J23116+B0032+cI+B0015 | 974 | |
| 43 | BBa_K2120325 | Device | J23106+B0034+cI+B0015 | 973 | |
| 44 | BBa_K2120326 | Device | J23106+B0032+tetR+B0015 | 884 | |
| 45 | BBa_K2120327 | Device | J23106+B0032+cI+B0015 | 974 | |
| Application Circuit | |||||
| 46 | BBa_K2120400 | Composite | B0015+tetR+B0034+J23119+Ptet+B0034+mazF+B0015 | 1446 | |
| 47 | BBa_K2120401 | Composite | B0015+tetR+B0034+J23119+Ptet+B0032+mazF+B0015 | 1447 | |
| 48 | BBa_K2120402 | Composite | B0015+tetR+B0034+J23116+Ptet+B0034+mazF+B0015 | 1446 | |
| 49 | BBa_K2120403 | Composite | B0015+tetR+B0034+J23109+Ptet+B0034+mazF+B0015 | 1446 | |
| 50 | BBa_K2120404 | Composite | B0015+tetR+B0034+J23106+Ptet+B0034+mazF+B0015 | 1446 | |
| 51 | BBa_K2120405 | Composite | B0015+cI+B0034+J23119+PR+B0034+mazF+B0015 | 1531 | |
| 52 | BBa_K2120406 | Composite | B0015+cI+B0034+J23119+PR+B0032+mazF+B0015 | 1532 | |
| 53 | BBa_K2120407 | Composite | B0015+cI+B0034+J23116+PR+B0034+mazF+B0015 | 1531 | |
| 54 | BBa_K2120408 | Composite | B0015+cI+B0034+J23109+PR+B0034+mazF+B0015 | 1531 | |
| 55 | BBa_K2120409 | Composite | B0015+cI+B0034+J23106+PR+B0034+mazF+B0015 | 1531 | |
| 56 | BBa_K2120410 | Composite | B0015+tetR+B0034+J23116+Ptet+B0032+mazF+B0015 | 1447 | |
| 57 | BBa_K2120411 | Composite | B0015+tetR+B0034+J23109+Ptet+B0032+mazF+B0015 | 1447 | |
| 58 | BBa_K2120412 | Composite | B0015+tetR+B0034+J23106+Ptet+B0032+mazF+B0015 | 1447 | |
| 59 | BBa_K2120413 | Composite | B0015+cI+B0034+J23116+PR+B0032+mazF+B0015 | 1532 | |
| 60 | BBa_K2120414 | Composite | B0015+cI+B0034+J23109+PR+B0032+mazF+B0015 | 1532 | |
| 61 | BBa_K2120415 | Composite | B0015+cI+B0034+J23106+PR+B0032+mazF+B0015 | 1532 | |
| 62 | BBa_K2120416 | Composite | B0015+tetR+B0034+J23119+Ptet+B0034+mRFP+B0015 | 1816 | |
| 63 | BBa_K2120418 | Composite | B0015+tetR+B0034+J23116+Ptet+B0034+mRFP+B0015 | 1816 | |
| 64 | BBa_K2120419 | Composite | B0015+tetR+B0034+J23109+Ptet+B0034+mRFP+B0015 | 1816 | |
| 65 | BBa_K2120420 | Composite | B0015+tetR+B0034+J23106+Ptet+B0034+mRFP+B0015 | 1816 | |
| 66 | BBa_K2120421 | Composite | B0015+cI+B0034+J23119+PR+B0032+mRFP+B0015 | 1902 | |
| 67 | BBa_K2120423 | Composite | B0015+cI+B0034+J23116+PR+B0032+mRFP+B0015 | 1902 | |
| 68 | BBa_K2120424 | Composite | B0015+cI+B0034+J23109+PR+B0032+mRFP+B0015 | 1902 | |
| 69 | BBa_K2120425 | Device | B0015+cI+B0034+J23106+PR+B0032+mRFP+B0015 | 1902 | |
