Difference between revisions of "Team:Kyoto/Experiments"
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| + | </h1>Protocols</h1> | ||
| + | <h2>Ligation</h2> | ||
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| + | Ligation was performed according to Ligation high Ver.2’s protocol. | ||
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| + | 1. unfreeze competent cells on hands. put them on ice. | ||
| + | 2. add DNA sample (1~20ul) to competent cells. leave them on ice for 30 minutes | ||
| + | 3. keep them in heating block 45 seconds, then on ice for 30 minutes. | ||
| + | 4. add 40~200ul SOC liquid culture medium, then incubate it under shaking culture for 1hour 37℃. | ||
| + | 5. seed it onto LB+antibiotics +/- culture. incubate it for 24 hour at 37℃. | ||
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| + | |||
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<h1>header 1</h1> | <h1>header 1</h1> | ||
<h2>header 2</h2> | <h2>header 2</h2> | ||
Revision as of 09:14, 12 October 2016
Ligation
Ligation was performed according to Ligation high Ver.2’s protocol. 1. unfreeze competent cells on hands. put them on ice. 2. add DNA sample (1~20ul) to competent cells. leave them on ice for 30 minutes 3. keep them in heating block 45 seconds, then on ice for 30 minutes. 4. add 40~200ul SOC liquid culture medium, then incubate it under shaking culture for 1hour 37℃. 5. seed it onto LB+antibiotics +/- culture. incubate it for 24 hour at 37℃.header 1
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