Difference between revisions of "Team:Stony Brook/Notebook/Cancer-W14"

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<h2 > 10/2 </h2>
 
<h2 > 10/2 </h2>
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<h4> Transformations </h4>
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<ol>
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<li> Thaw TSS cells on ice</li>
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<li> Add 2ul of prepped plasmid. Pipette to mix </li>
 +
<li> Sit for 30 minutes on ice </li>
 +
<li> Incubate cells for 30s at 43°C </li>
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<li> Incubate on ice for 2 minutes </li>
 +
<li> Add 1ml of 50C </li>
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<li> Incubate for 30 minutes at 37°C. Shake </li>
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<li> Spread 100-300ul on plates </li>
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<li> Grow overnight at 37°C </li>
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<li> Save the rest as liquid cultures </li>
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</ol>
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<br>
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<ul>
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<li> Plate 1: pEPGAP </li>
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<li> Plate 2: pEPGAP/CRISBU </li>
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<li> Plate 3: pEPGAP-P </li>
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<li> Plate 4: </li>
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<li> Plate 5: pSBIA2/T1/T2 </li>
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<li> Plate 6: pSBIA2 </li>
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<li> Plate 7: pSBIA2-P </li>
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<li> Plate 8: </li>
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</ul>
 
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Revision as of 02:29, 12 October 2016

Week 14: 9/26 - 10/2

Week 14: 9/26 - 10/2



9/26



9/27



9/28



9/29



9/30



10/1



10/2

Transformations

  1. Thaw TSS cells on ice
  2. Add 2ul of prepped plasmid. Pipette to mix
  3. Sit for 30 minutes on ice
  4. Incubate cells for 30s at 43°C
  5. Incubate on ice for 2 minutes
  6. Add 1ml of 50C
  7. Incubate for 30 minutes at 37°C. Shake
  8. Spread 100-300ul on plates
  9. Grow overnight at 37°C
  10. Save the rest as liquid cultures

  • Plate 1: pEPGAP
  • Plate 2: pEPGAP/CRISBU
  • Plate 3: pEPGAP-P
  • Plate 4:
  • Plate 5: pSBIA2/T1/T2
  • Plate 6: pSBIA2
  • Plate 7: pSBIA2-P
  • Plate 8: