Difference between revisions of "Team:NCTU Formosa/Composite Part"

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Revision as of 14:06, 12 October 2016

Composite part

We have known the function of the three toxic peptides of Pantide, ω-hexatoxin-Hv1a, µ-segestritoxin-Sf1a, and Orally Active Insecticidal Peptide(OAIP) respectively and they are easy to express with the help of the bacteria E. coli BL21 Rosetta Gami strain. So, we then start to create BioBricks of these three toxins which we have obtained the nucleotide sequences. We use a strong T7 promoter that is suitable with the strain of E. coli we chose and also powerful at expressing proteins with multiple disulfide bonds, and we add His-tag for protein purification.

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