Difference between revisions of "Team:UrbanTundra Edmonton/Notebook"

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<p> Document the dates you worked on your project.</p>
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<li><b>July 20th, 2016</b>
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<ol>4 microlitres of milliq water with 1 microlitre of plasmid</ol>
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<ol>5 microlitres of no chlorite dismutase</ol>
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<ol>1 microlitre of cutsmart buffer (specifically 1.1111 microlitre)</ol>
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<ol>10 x cutsmart buffer into tubes makes 1x [ ]</ol>
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<h5>What should this page have?</h5>
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<ol>When adding BsaI, only touch the surface of the BsaI to the pipette tip. The reason: has glycerol which is sticky. Take 0.5 microlitre of BsaI.</ol>
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<ul>
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<ol>Mix sample very gently then put into a 37 degrees celsius incubator for 1 hour. </ol>
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<li>Chronological notes of what your team is doing.</li>
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<ol>Heat kill at 65 degrees celsius for 20 minutes. This kills the BsaI to prevent star activity. </ol>
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<li> Brief descriptions of daily important events.</li>
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<ol>Pulse down tubes then add 8 microlitre of milliq water. </ol>
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<li>Pictures of your progress. </li>
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<ol>Add 2 microlitres of T4DNA ligase buffer. </ol>
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<li>Mention who participated in what task.</li>
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<ol>Add 0.5 microlitre of ligase then mix gently and incubate at room temperature for 30 minutes or longer. </ol>
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<h5>Inspiration</h5>
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<p>You can see what others teams have done to organize their notes:</p>
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<ul>  
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<li><a href="https://2014.igem.org/Team:ATOMS-Turkiye/Notebook">2014 ATOMS-Turkiye</a></li>
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<li><a href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_project.html#tab_notebook">2014 Tec Monterrey</a></li>
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<li><a href="https://2014.igem.org/Team:Kyoto/Notebook/Magnetosome_Formation#title">2014 Kyoto</a></li>
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<li><a href="https://2014.igem.org/Team:Cornell/notebook">2014 Cornell</a></li>
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</ul>
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Revision as of 06:24, 16 October 2016

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  • July 20th, 2016
      4 microlitres of milliq water with 1 microlitre of plasmid
      5 microlitres of no chlorite dismutase
      1 microlitre of cutsmart buffer (specifically 1.1111 microlitre)
      10 x cutsmart buffer into tubes makes 1x [ ]
      When adding BsaI, only touch the surface of the BsaI to the pipette tip. The reason: has glycerol which is sticky. Take 0.5 microlitre of BsaI.
      Mix sample very gently then put into a 37 degrees celsius incubator for 1 hour.
      Heat kill at 65 degrees celsius for 20 minutes. This kills the BsaI to prevent star activity.
      Pulse down tubes then add 8 microlitre of milliq water.
      Add 2 microlitres of T4DNA ligase buffer.
      Add 0.5 microlitre of ligase then mix gently and incubate at room temperature for 30 minutes or longer.