Difference between revisions of "Team:ShanghaitechChina/Notebook"

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<h4><b>Part III  Characterization of biofilm in CongoRed Plates.</b></h4>
 
<h4><b>Part III  Characterization of biofilm in CongoRed Plates.</b></h4>
 
Take the activated CsgA-Histag mutant E.coli in small amount into the plates and cultivate them for 48 hours and then the CongoRed plates will show red biofilm vividly if the experiment succeed.<p></p>
 
Take the activated CsgA-Histag mutant E.coli in small amount into the plates and cultivate them for 48 hours and then the CongoRed plates will show red biofilm vividly if the experiment succeed.<p></p>
<font color="red">The following steps work for the experiments which can dye the biofilm with the crystal violet.</font>
+
<font color="red">The following steps work for the experiments which can dye the biofilm with the crystal violet.</font><p></p>
 
         i.Add 400 ul 0.1% crystal violet dye to the medium, and hatch for 10 to15 minutes.<p></p>
 
         i.Add 400 ul 0.1% crystal violet dye to the medium, and hatch for 10 to15 minutes.<p></p>
 
ii.Wash the medium with ddH2O for 3 to 4 times with the aim of clean the dye;<p></p>
 
ii.Wash the medium with ddH2O for 3 to 4 times with the aim of clean the dye;<p></p>

Revision as of 16:25, 18 October 2016

igem2016:ShanghaiTech