Difference between revisions of "Team:ShanghaitechChina/Proof"
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<h1 align="center">Abstract</h1> | <h1 align="center">Abstract</h1> | ||
| − | Solar Hunter is an artificial hydrogen production system made up of biofilm-anchored nanorods which efficiently convert photons to electrons, and engineered strain expressing [FeFe] Hydrogenase as the acceptor of the electrons to produce hydrogen. The process of making the Solar Hunter entails the testing of each component ’s function separately. | + | <h2>Solar Hunter is an artificial hydrogen production system made up of biofilm-anchored nanorods which efficiently convert photons to electrons, and engineered strain expressing [FeFe] Hydrogenase as the acceptor of the electrons to produce hydrogen. The process of making the Solar Hunter entails the testing of each component ’s function separately.</h2> |
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<div id="CNanomaterial"> | <div id="CNanomaterial"> | ||
| − | < | + | <h2>a. We, first, need to obtain a suitable nanomaterial that absorbs solar energy and convert it into electrons. The synthesis and characterization data is shown in our webpage, <b><a href="https://2016.igem.org/Team:ShanghaitechChina/Nanomaterials">nanomaterials</a></b> </h2> |
<p></p></div> | <p></p></div> | ||
<div id="CBiofilm"> | <div id="CBiofilm"> | ||
| − | < | + | <h2>b. To allow easy recycling and points of aggregation for the small material for a higher efficiency, we utilized biofilm to bind nanomaterials. Please refer to <a href="https://2016.igem.org/Team:ShanghaitechChina/Biofilm">Biofilm</a> for the data of the successful construction and the characterization of the binding of nanomaterials to biofilm.</h2><p></p></div> |
<div id="CHydrogenase"> | <div id="CHydrogenase"> | ||
| − | < | + | <h2>c. Finally, high-activity hydrogenase is necessary. We have successfully produced hydrogen many times with our system. Please refer to Hydrogenase for data. Reference <a href="https://2016.igem.org/Team:ShanghaitechChina/Hydrogen">Hydrogenase </h2><p></p></a> |
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<h1 align="center">Main points we achieved in our project</h1> | <h1 align="center">Main points we achieved in our project</h1> | ||
| − | 1. Successful synthesis of CdS with desired features.<p></p> | + | <h2>1. Successful synthesis of CdS with desired features.</h2><p></p> |
| − | 2. Confirmed the firm binding of nanomaterials on biofilm composed by CsgA-Histag fused protein. | + | <h2>2. Confirmed the firm binding of nanomaterials on biofilm composed by CsgA-Histag fused protein.Tested the <a href="http://parts.igem.org/Part:BBa_K2132001">BioBrick BBa_K2132001</a> adhesion to multi-well and micro-beads, and its binding to nano materials within the hydrogen production system.</h2><p></p> |
| − | Tested the <a href="http://parts.igem.org/Part:BBa_K2132001">BioBrick BBa_K2132001</a> adhesion to multi-well and micro-beads, and its binding to nano materials within the hydrogen production system.<p></p> | + | <h2>3. Transplanted of all the necessary subunits from the gene clusters of [FeFe]-hydrogenase from Clostridium. acetobutylicum onto one single plasmid to allow reliable expression.</h2><p></p> |
| − | 3. Transplanted of all the necessary subunits from the gene clusters of [FeFe]-hydrogenase from Clostridium. acetobutylicum onto one single plasmid to allow reliable expression.<p></p> | + | |
Revision as of 16:44, 18 October 2016
