Difference between revisions of "Team:British Columbia/Project/Bio-Pathways"

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                     <h2>Key Achievements</h2>
 
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                     <li>Cloned the operon encoding β-carotene production into pSB1C3.</li>
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<li>Established growth curves for DH5α Escherichia coli cells encoding  β-carotene. </li>
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<li>Compared β-carotene production between the K12 (MG1655) and DH5α E. coli strains. </li>
 
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Revision as of 21:45, 18 October 2016

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Bio-Pathways

Biosynthetic Pathways

Abstract

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Key Achievements

  • Cloned the operon encoding β-carotene production into pSB1C3.
  • Established growth curves for DH5α Escherichia coli cells encoding β-carotene.
  • Compared β-carotene production between the K12 (MG1655) and DH5α E. coli strains.

    • Introduction

    Escherichia coli is commonly used as a chassis for the biosynthesis of valuable chemicals. Advantages to its use include fast growth kinetics, ease of acquiring high density cultures, ability to grow on many types of media that can be made from inexpensive reagents, and tractability for genetic manipulation (Rosano and Ceccarelli 2014). As such, many past iGEM teams have chosen to engineer biosynthetic pathways into E. coli for production of a wide range of commercially-desired products such as biofuels, violacein, astaxanthin, and its precursor β-carotene.

    This year, we intend to build a platform for producing valuable chemicals more sustainably by bridging lignocellulose processing directly to product manufacture. For our project, we decided to engineer E. coli to produce β-carotene in tandem to its growth with C.crescentus. In particular, we designed our system so that E. coli metabolizes the simple sugars released from cellulosic degradation accomplished by cellulase-expressing C. crescentus, to in turn produce β-carotene as a secondary metabolite.

    β-carotene is a carotenoid found in many colored fruits and vegetables. It is the biosynthetic precursor to vitamin A which has roles in gene expression, vision, maintenance of body linings and skin, immune defenses, growth of the body, and normal development of cells (Sizer and Whitney 2016). Aside from being a precursor, β-carotene also has functions as a potent quencher of singlet toxic oxygen species, and can act as an antioxidant that scavenges free radicals in human low density lipoprotein (LDL), high density lipoprotein (HDL), and cell membranes (Bendich 2004).

    We chose to produce β-carotene as a proof-of-concept approach to validating the functionality of our system. However, this platform can be applied to the production of higher value chemicals. We anticipate that directly linking chemical production to treatment of lignocellulosic biomass, in the form of a microbial consortium, would result in both effectively reducing the costs associated with chemical production and valorizing lignocellulosic waste.

    Design

    WE MUST ADD THIS SECTION :)

    Methods

    Results

    Conclusion

    References

    Check out other parts of our project below!