Difference between revisions of "Team:Waterloo"
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| − | <h4> We initiate a [PSI+] response in S. cerevisiae using a plasmid that will overexpress Sup35-NM. </h4></div> | + | <h4 id="texticles1"> We initiate a [PSI+] response in S. cerevisiae using a plasmid that will overexpress Sup35-NM. </h4></div> |
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| − | <h4> We insert a premature stop codon into an N-terminal CFP tag that will be read through and fluoresce during a [PSI+] response</h4></div> | + | <h4 id="texticles2"> We insert a premature stop codon into an N-terminal CFP tag that will be read through and fluoresce during a [PSI+] response</h4></div> |
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| − | <h4>We control read through rates by choosing the location of the premature stop codon, and can therefore control protein production in [PSI+]. We use Hsp104, a chaperone protein in S. cerevisiae, to demonstrate that our set-up phenotypically responds to the stop codon readthrough. </h4> </div> | + | <h4 id="texticles3">We control read through rates by choosing the location of the premature stop codon, and can therefore control protein production in [PSI+]. We use Hsp104, a chaperone protein in S. cerevisiae, to demonstrate that our set-up phenotypically responds to the stop codon readthrough. </h4> </div> |
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Revision as of 03:05, 19 October 2016
OFF to priON
Using stop codon read-through and CRISPR to explore S. cerevisiae prion mechanisms
Furthering the advancement of research into prions and neurodegenerative diseases
We initiate a [PSI+] response in S. cerevisiae using a plasmid that will overexpress Sup35-NM.
We insert a premature stop codon into an N-terminal CFP tag that will be read through and fluoresce during a [PSI+] response
We control read through rates by choosing the location of the premature stop codon, and can therefore control protein production in [PSI+]. We use Hsp104, a chaperone protein in S. cerevisiae, to demonstrate that our set-up phenotypically responds to the stop codon readthrough.
