Difference between revisions of "Team:ShanghaitechChina/Attributions"

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<img class="imgnav" src="https://static.igem.org/mediawiki/2016/a/a9/T--ShanghaitechChina--title-Attribution.png">
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<img class="imgnav" src="http://2018.iGEM.org/wiki/images/a/a9/T--ShanghaitechChina--title-Attribution.png">
  
 
<div id="Connection" class="content">
 
<div id="Connection" class="content">
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<div class="col-lg-6">
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<center><p style="font-size:28px">
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<center><p style="font-size:18px">
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   Logistics: Chen Yifan, Liu Yi
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   Logistics: Yifan Chen, Yi Liu
 
   </p></center>
 
   </p></center>
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   <center><p style="font-size:28px">
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   <center><p style="font-size:18px">
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   Abstract: Liu Yi, Ba Fang, Gu Shijie
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   Abstract: Yi Liu, Fang Ba, Shijie Gu
 
   </p></center>
 
   </p></center>
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   <center><p style="font-size:28px">
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   <center><p style="font-size:18px">
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   Parts: Gu Shijie; Chen Yifan
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   Parts: Shijie Gu, Yifan Chen
 
   </p></center>
 
   </p></center>
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   <center><p style="font-size:28px">
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   <center><p style="font-size:18px">
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   Webpage construction and design: Shen Yifei
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   Webpage construction and design: Yifei Shen
 
   </p></center>
 
   </p></center>
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  <center><p style="font-size:28px">
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  <center><p style="font-size:18px">
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   wiki Big Picture: Bafang, Gu Shijie
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   wiki Big Picture: Lechen Qian, Fang Ba
 
   </p></center>
 
   </p></center>
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<center> <p style="font-size:28px">
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<center> <p style="font-size:18px">
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   wiki design: Shen Yifei
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   wiki design: Yifei Shen
 
   </p></center>
 
   </p></center>
 
</div>
 
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<center> <p style="font-size:28px">
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   wiki home background: Ye Qinying
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   wiki home background: Qinying Ye
 
   </p></center>
 
   </p></center>
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  <center> <p style="font-size:28px">
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  <center> <p style="font-size:18px">
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   wiki home video: Gu Shijie, Zhang Jiaming, Ye Qinying, Qian Lechen.
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   wiki home video: Shijie Gu, Jiaming Zhang, Qinying Ye, Lechen Qian.
 
   </p></center>
 
   </p></center>
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<center><p style="font-size:28px">
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<center><p style="font-size:18px">
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   wiki logo: Ye Qinying
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   wiki logo: Qinying Ye
 
   </p></center>
 
   </p></center>
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  <center> <p style="font-size:28px">
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  <center> <p style="font-size:18px">
 
   Wiki team avatar: JSNU
 
   Wiki team avatar: JSNU
 
   </p></center>
 
   </p></center>
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  <center> <p style="font-size:28px">
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  <center> <p style="font-size:18px">
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   Banner&amp;Post: Ba Fang
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   Banner&amp;Post: Fang Ba
 
   </p></center>
 
   </p></center>
  
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<h3><b>Special Notes:</b>iGem is the first lab experience for many members on our team. It was the training and guidance of the host labs that put us onto the right track for carrying out the independent project, Solar Hunter. On this page, we show our acknowledgement sincerely by clearly attributing the help from the graduate students and instructors at the beginning of each section, as different graduate students were involved in different aspects of the project.
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<h5><b>Special Notes:</b>iGEM is the first lab experience for many members on our team. It was the training and guidance of the host labs that put us onto the right track for carrying out the independent project, Solar Hunter. On this page, we show our acknowledgement sincerely by clearly attributing the help from the graduate students and instructors at the beginning of each section, as different graduate students were involved in different aspects of the project.
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</h3></div>
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</h5></div>
 
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                       <center>  <p style="font-size:28px">LiuYi,BaFang,Zhu Haolong,Wang Qiaoqi</p></center>
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                       <center>  <p style="font-size:18px">Yi Liu,BaFang,Zhu Haolong,Qiaoqi Wang</p></center>
  
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<h3><b>Special Notes:</b>We applied for the flow cytometry through the manager teacher Ren Xiaoyue. She also tutored us the basic operation and the preparation of our samples. We prepared the samples by ourselves.</h3>
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<h5><b>Special Notes:</b>We applied for the flow cytometry through the manager teacher Ren Xiaoyue. She also tutored us the basic operation and the preparation of our samples. We prepared the samples by ourselves.</h5>
 
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<div class="col-lg-6">
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<center> <p style="font-size:28px">
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<center> <p style="font-size:18px">
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   Clone Design: Sun Yilin, Chen Yifan, Nie Yan, LiuYi
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   Clone Design: Yilin Sun, Yifan Chen, Yan Nie, Yi Liu
 
   </p></center>
 
   </p></center>
  
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  <center> <p style="font-size:28px">
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  <center> <p style="font-size:18px">
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   Sequencing Confirm: LiuYi, Chen Yifan
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   Sequencing Confirm: Yi Liu, Yifan Chen
 
   </p></center>
 
   </p></center>
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<center>  <p style="font-size:28px">
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<center>  <p style="font-size:18px">
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   Clone of histag-TEV-hydA-SpyCatcher into pACE:Yang Haonan, Liu Yi, Ba Fang
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   Clone hydrogenase gene clusters:Haonan Yang, Yi Liu, Fang Ba, Yifan Chen
 
   </p></center>
 
   </p></center>
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   <center><p style="font-size:28px">
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   <center><p style="font-size:18px">
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  Clone of histag-TEV-hydA-SpyTag into pACE: Yang Haonan, Liu Yi, Ba Fang
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   Cre recombination &amp; Result Demonstration: Bafang, Yi Liu
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  </p></center>
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<center><p style="font-size:28px">
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  Clone of hydE into pDC: Chen Yifan, Liu Yi, Ba Fang
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  </p></center>
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    <center><p style="font-size:28px">
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  Clone of hydF into pDK: Ba Fang, Liu Yi
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  </p></center>
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  <center><p style="font-size:28px">
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  Clone of hydG into pDS: Liu Yi, Ba Fang
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  </p></center>
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  <center><p style="font-size:28px">
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   Cre recombination &amp; Result Demonstration: Bafang, LiuYi
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   </p></center>
 
   </p></center>
  
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  <center> <p style="font-size:28px">
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  <center> <p style="font-size:18px">
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   Hydrogen Assay: LiuYi, Ba Fang, Mi Qixi, Ding Zetao
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   Hydrogen Assay: Yi Liu, Fang Ba, Qixi Mi, Zetao Ding
 
   </p></center>
 
   </p></center>
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  <center> <p style="font-size:28px">
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  <center> <p style="font-size:18px">
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   Preparation of PirLC/DH5a/BL21(DE3) chemistry component cell : LiuYi
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   Preparation of component cell : Yi Liu, Yifan Chen
 
   </p></center>
 
   </p></center>
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  <center><p style="font-size:28px">
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   Preparation of DH5a electronic component cell : Chen Yifan
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<center> <p style="font-size:18px">
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   HydA Induction, Purification and WB Demonstration: Zhu Haolong, Qiaoqi Wang
 
   </p></center>
 
   </p></center>
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<center> <p style="font-size:28px">
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   <center><p style="font-size:18px">
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  HydA Induction and Synthesis: Zhu Haolong, Wang Qiaoqi
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   Wiki of Hydrogenase : Yi Liu, Shijie Gu, Fang Ba
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  </p></center>
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   <center><p style="font-size:28px">
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  HydA Purification: Zhu Haolong, Wang Qiaoqi
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  </p></center>
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  <center><p style="font-size:28px">
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  HydA Assay &amp; WB Demonstration: Zhu Haolong, Wang Qiaoqi
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  </p></center>
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  <center><p style="font-size:28px">
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   Wiki of Hydrogenase : Liu Yi, Gu Shijie, Ba Fang
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   </p></center>
 
   </p></center>
  
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<div class="col-lg-12">
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<h3> <b>Special Notes:</b>In this part Nie Yan provided us with the original bacteria of PirLC to make the component cell and the transformed PirLC with the plasmid backbone.The molecular cloning in this section was done in Nie Yan’s Lab, SIAIS. The help of Nie Yan was involved in the the beginning, but the principle is that all of the experiments are done by ourselves.  Sun Yilin provided us with the tutorials of Snapgene and primer design.The sequencing is provided by <a href="http://www.tsingke.net/">Tsingke Sequencing</a> and <a href="http://www.genebioseq.com/">Jie Li Sequencing</a>.Mi Qixi is a PI from School of Physical Science and Technology. Ding Zetao is his Ph.D student. In the hydrogen production assay, they provided us with the instrument/apparatus he used for hydrogen production assay in his lab. He trained for using the apparatus and most of the experiments were conducted by us.</h3></div>
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<h5> <b>Special Notes:</b>In this part Yan Nie provided us with the original bacteria of PirLC to make the component cell and the transformed PirLC with the plasmid backbone.The molecular cloning in this section was done in Yan Nie’s Lab, SIAIS. The help of Yan Nie was involved in the the beginning, but the principle is that all of the experiments are done by ourselves.  Yilin Sun provided us with the tutorials of Snapgene and primer design.The sequencing is provided by <a href="http://www.tsingke.net/">Tsingke Sequencing</a> and <a href="http://www.genebioseq.com/">Jie Li Sequencing</a>.Mi Qixi is a PI from School of Physical Science and Technology. Ding Zetao is his Ph.D student. In the hydrogen production assay, they provided us with the instrument/apparatus he used for hydrogen production assay in his lab. He trained for using the apparatus and most of the experiments were conducted by us.</h5></div>
 
</div></div>
 
</div></div>
  
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<div class="col-lg-12">
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  <center><p style="font-size:28px">
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  <center><p style="font-size:18px">
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   Clone of his-CsgA-SpyCatcher-his :Gu Shijie, An Bolin (Graduate Instructor/Advisor)
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   Clone of a library of CsgA fused protein and sequencing confirm :Shijie Gu, An Bolin (Graduate Instructor/Advisor)
 
   </p></center>
 
   </p></center>
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   <center><p style="font-size:28px">
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  Clone of his-CsgA-SpyCatcher: Gu Shijie
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   <center><p style="font-size:18px">
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  </p></center>
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   <center><p style="font-size:28px">
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   Clone of CsgA-his: A gift of Timothy K. Lu Lab at MIT Synthetic Biology Center
 
   Clone of CsgA-his: A gift of Timothy K. Lu Lab at MIT Synthetic Biology Center
 
   </p></center>
 
   </p></center>
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   <center><p style="font-size:28px">
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   <center><p style="font-size:18px">
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   Clone of mcherry-SpyTag: Gu Shijie
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   Clone of mcherry-SpyTag: Shijie Gu
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  </p></center>
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  <center><p style="font-size:28px">
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  Clone of CsgA-SpyCatcher-his: Gu Shijie
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  </p></center>
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  <center><p style="font-size:28px">
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  Clone of CsgA-SpyCatcher: Gu Shijie
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  </p></center>
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  <center><p style="font-size:28px">
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  Clone of CsgA: Gu Shijie
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  </p></center>
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  <center><p style="font-size:28px">
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  Sequencing Confirm:  Gu Shijie, An Bolin
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   </p></center>
 
   </p></center>
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   <center><p style="font-size:28px">
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 +
   <center><p style="font-size:18px">
 
   mCherry-SpyCatcher strain: A gift of Zhong Chao Lab at ShanghaiTech University
 
   mCherry-SpyCatcher strain: A gift of Zhong Chao Lab at ShanghaiTech University
 
   </p></center>
 
   </p></center>
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   <center><p style="font-size:28px">
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   <center><p style="font-size:18px">
 
   ΔCsgA strain: A gift of Timothy K. Lu Lab at MIT Synthetic Biology Center
 
   ΔCsgA strain: A gift of Timothy K. Lu Lab at MIT Synthetic Biology Center
 
   </p></center>
 
   </p></center>
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   <center><p style="font-size:28px">
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   <center><p style="font-size:18px">
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   Colony PCR: Gu Shijie, Qian Lechen
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   Colony PCR: Shijie Gu, Lechen Qian
 
   </p></center>
 
   </p></center>
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   <center><p style="font-size:28px">
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   <center><p style="font-size:18px">
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   Congo Red Assay(plate): Zheng Weitong, Qian Lechen
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   Biofilm Characterization: Lechen Qian, Jiaming Zhang
 
   </p></center>
 
   </p></center>
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   <center><p style="font-size:28px">
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   <center><p style="font-size:18px">
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   Congo Red Assay(solution): Qian Lechen,Zheng Weitong,Zhuang Lei
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   Biofilm culture: Lechen Qian, Zhuanglei, Weitong Zheng
 
   </p></center>
 
   </p></center>
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   <center><p style="font-size:28px">
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   <center><p style="font-size:18px">
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  Expression and purification of mcherry-spycatcher: Zhuang Lei, Qian Lechen, Liu Yi, Zhang Jiaming
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 +
  <center><p style="font-size:18px">
 +
  Different nanomaterials supply for characterization: Wang Xinyu, Pu Jiahua (Graduate Instructor/Advisor), Jiaming Zhang, Lei Zhuang
 
   </p></center>
 
   </p></center>
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   <center><p style="font-size:28px">
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   <center><p style="font-size:18px">
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   Expression and purification of mcherry-spytag: Zhuang Lei, An Bolin, Qian Lehchen
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   Biofilm expression optimization: Lechen Qian
 
   </p></center>
 
   </p></center>
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  <center><p style="font-size:28px">
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  <center><p style="font-size:18px">
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   Biofilm culture: Qian Lechen, Zhuanglei, Zheng Weitong
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   biofilm data analysis and result demostration: Lechen Qian
 
   </p></center>
 
   </p></center>
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  <center><p style="font-size:28px">
 
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  Crystal violet assay: Qian Lechen, Zhang Jiaming
 
−
  </p></center>
 
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<center><p style="font-size:28px">
 
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  TEM sample preparation: Qian Lechen, Zhang Jiaming，Zhuanglei, Wang Xinyu (Graduate Instructor/Advisor)
 
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  </p></center>
 
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</div>
 
  
−
 
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   <center><p style="font-size:18px">
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<div class="col-lg-6">
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   notebook data and protocal collection ：Weitong Zheng
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<center><p style="font-size:28px">
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  TEM: Qian Lechen，Zhang Jiaming, An Bolin, Wang Xinyu
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  </p></center>
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   <center><p style="font-size:28px">
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  Fluorescence Microscopy: Qian Lechen, An Bolin
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  </p></center>
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  <center><p style="font-size:28px">
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  Quantum dots(QD) binding test: Qian Lechen,Zhang Jiaming
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  </p></center>
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  <center><p style="font-size:28px">
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  Different QDs supply for characterization: Wang Xinyu, Pu Jiahua (Graduate Instructor/Advisor), Zhang Jiaming, Zhuang Lei
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  </p></center>
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  <center><p style="font-size:28px">
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  Nanorods binding test: Qian Lechen
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  </p></center>
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  <center><p style="font-size:28px">
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  Nanorods supply for binding: Zhang Jiaming
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  </p></center>
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  <center><p style="font-size:28px">
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  mcherry-SpyCatcher and CsgA-SpyTag incubation and sample preparation: Qian Lechen
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  </p></center>
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  <center><p style="font-size:28px">
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  mcherry-SpyTag and CsgA-SpyCatcher incubation and sample preparation: Qian Lechen
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  </p></center>
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  <center><p style="font-size:28px">
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  Biofilm expression optimization: Qian Lechen
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  </p></center>
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  <center><p style="font-size:28px">
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  Confocal fluorescence microscope: Qian Lechen,Li Xiaoming(instructor)
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  </p></center>
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  <center><p style="font-size:28px">
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  biofilm data analysis and result demostration:Qian Lechen
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  </p></center>
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  <center><p style="font-size:28px">
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  Biofilm microsphere coculture:Qian Lechen
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  </p></center>
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  <center><p style="font-size:28px">
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  Reagent and material preparation:Zheng Weitong, Qian Lechen, Gu Shijie, Zhang Jiaming, Zhuanglei
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  </p></center>
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  <center><p style="font-size:28px">
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   notebook data and protocal collection ：Zheng Weitong
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   </p></center>
 
   </p></center>
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   <center><p style="font-size:28px">
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   <center><p style="font-size:18px">
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   wiki of biofilm: Qian Lechen, Gu Shijie
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   wiki of biofilm: Lechen Qian, Shijie Gu
 
   </p></center>
 
   </p></center>
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   <center><p style="font-size:28px">
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   <center><p style="font-size:18px">
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   wiki biofilm picture: Qian Lechen
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   wiki biofilm picture: Lechen Qian
 
   </p></center>
 
   </p></center>
 
</div>
 
</div>
  
 
<div class="col-lg-12">
 
<div class="col-lg-12">
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<h3><b>Special Notes:</b>An Bolin is the leading graduate instructor/advisor in this section. He provided basic cloning training (PCR, restriction, ligation, Gibson, electrophoresis, Colony PCR, blast) at the beginning of the cloning of his-CsgA-SpyCatcher-his, the purification of proteins at the subsection of Expression and Purification of mCherry-SpyTag, and consult throughout the summer and fall. In addition, all florescent microscope experiments were conducted with the supervision of An Bolin. TEM is conducted with the help of Wang Xinyu. However, the preparation of the samples were done by ourselves.
+
<h5><b>Special Notes:</b>An Bolin is the leading graduate instructor/advisor in this section. He provided basic cloning training (PCR, restriction, ligation, Gibson, electrophoresis, Colony PCR, blast) at the beginning of the cloning of his-CsgA-SpyCatcher-his, the purification of proteins at the subsection of Expression and Purification of mCherry-SpyTag, and consult throughout the summer and fall. In addition, all florescent microscope experiments were conducted with the supervision of An Bolin. TEM is conducted with the help of Wang Xinyu. However, the preparation of the samples were done by ourselves.
−
</h3></div>
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</h5></div>
 
</div></div>
 
</div></div>
  
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<div class="col-lg-12">
 
<div class="col-lg-12">
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<center><p style="font-size:28px">
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<center><p style="font-size:18px">
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   Quantum dots, nanorods and ligand synthesis: Zhang Jiaming, Zhuang Lei, Wang Kang
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   Quantum dots, nanorods and ligand synthesis: Jiaming Zhang, Lei Zhuang, Kang Wang
 
   </p></center>
 
   </p></center>
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<center>  <p style="font-size:28px">
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<center>  <p style="font-size:18px">
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   Quantum dot and nanorods characterization:Zhang Jiaming
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   Quantum dot and nanorods characterization:Jiaming Zhang
 
   </p></center>
 
   </p></center>
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  <center> <p style="font-size:28px">
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  <center> <p style="font-size:18px">
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   wiki of Quantum Dots: Zhang Jiaming, Zhuang lei
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   wiki of Quantum Dots: Jiaming Zhang, Lei Zhuang
 
   </p></center>
 
   </p></center>
 
</div>
 
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<div class="col-lg-12">
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<h3><b>Special Notes:</b>Pu Jiahua and Wang Xinyu are two graduate instructors/advisors in the QD section. Pu provided guidance on our experiments including the synthesis of CdSe QDs and CdS NRs and  UV-Vis spectrometer characterization. Wang helped in the synthesis of HS-NTA synthesis, TEM characterization and PL spectrometer characterization.
+
<h5><b>Special Notes:</b>Pu Jiahua and Wang Xinyu are two graduate instructors/advisors in the QD section. Pu provided guidance on our experiments including the synthesis of CdSe QDs and CdS NRs and  UV-Vis spectrometer characterization. Wang helped in the synthesis of HS-NTA synthesis, TEM characterization and PL spectrometer characterization.
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</h3></div></div></div>
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</h5></div></div></div>
  
  
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<center><p style="font-size:28px">
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<center><p style="font-size:18px">
 
   Team Collaboration with iGEM at Jiangsu Normal University: Yi Liu; Fang Ba; Haolong Zhu
 
   Team Collaboration with iGEM at Jiangsu Normal University: Yi Liu; Fang Ba; Haolong Zhu
 
   </p></center>
 
   </p></center>
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   <center><p style="font-size:28px">
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   <center><p style="font-size:18px">
 
   Team Collaboration with iGEM at Tsinghua University: Lechen Qian; Haolong Zhu
 
   Team Collaboration with iGEM at Tsinghua University: Lechen Qian; Haolong Zhu
 
   </p></center>
 
   </p></center>
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   <center><p style="font-size:28px">
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   <center><p style="font-size:18px">
 
   Public Questionnaire Design: Haolong Zhu; Yi Liu; Lechen Qian; Qiaoqi Wang
 
   Public Questionnaire Design: Haolong Zhu; Yi Liu; Lechen Qian; Qiaoqi Wang
 
   </p></center>
 
   </p></center>
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   <center><p style="font-size:28px">
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   <center><p style="font-size:18px">
 
   Public Questionnaire Analysis: Haolong Zhu
 
   Public Questionnaire Analysis: Haolong Zhu
 
   </p></center>
 
   </p></center>
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<center><p style="font-size:28px">
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<center><p style="font-size:18px">
 
   Interview with Industrial Community: Haolong Zhu; Yi Liu
 
   Interview with Industrial Community: Haolong Zhu; Yi Liu
 
   </p></center>
 
   </p></center>
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   <center><p style="font-size:28px">
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   <center><p style="font-size:18px">
 
   Interview with Academic Scientists: Haolong Zhu; Lechen Qian; Yi Liu; Jiaming Zhang; Qiaoqi Wang
 
   Interview with Academic Scientists: Haolong Zhu; Lechen Qian; Yi Liu; Jiaming Zhang; Qiaoqi Wang
 
   </p></center>
 
   </p></center>
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   <center><p style="font-size:28px">
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   <center><p style="font-size:18px">
 
   Interview with Administrative Authority: Haolong Zhu; Yi Liu; Lechen Qian; Jiaming Zhang
 
   Interview with Administrative Authority: Haolong Zhu; Yi Liu; Lechen Qian; Jiaming Zhang
 
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   Propagation of iGEM (Opening Day for Concordia International School Shanghai): Haolong Zhu; Shijie Gu; Weitong Zheng; Lechen Qian
 
   Propagation of iGEM (Opening Day for Concordia International School Shanghai): Haolong Zhu; Shijie Gu; Weitong Zheng; Lechen Qian
 
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   Wiki of Human Practice: Haolong Zhu
 
   Wiki of Human Practice: Haolong Zhu
 
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   Composition: Haolong Zhu
 
   Composition: Haolong Zhu
 
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   Photos: Yi Liu; Fang Ba; Haolong Zhu; Lechen Qian
 
   Photos: Yi Liu; Fang Ba; Haolong Zhu; Lechen Qian
 
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Revision as of 09:49, 19 October 2016

igem2016:ShanghaiTech