Difference between revisions of "Team:Stony Brook/Parts"

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<h5 style="font-family: Georgia;"> Cripto-1 Expression System (K1908001) </h5>
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<h3 style="font-family: Georgia;"> Cripto-1 Expression System (K1908001) </h3>
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This part is a semi-composite part that was synthesized by IDT which holds a <i>Saccharomyces cerevisiae</i> Kozak sequence, a coding sequence for the <i>Homo sapien</i> membrane protein Cripto-1, and an ADH1 Terminator region. The coding sequence was codon-optimized for expression in yeast using the proprietary codon optimization program on the Integrated DNA Technologies website. Our design was intended to induce a strong expression of Cripto-1 in yeast to test for membrane localization. We hope that our contribution of a eukaryotic receptor protein to the iGEM Registry is found to be useful and beneficial.
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Revision as of 06:11, 19 October 2016

New BioBricks



Cripto-1 Expression System (K1908001)


This part is a semi-composite part that was synthesized by IDT which holds a Saccharomyces cerevisiae Kozak sequence, a coding sequence for the Homo sapien membrane protein Cripto-1, and an ADH1 Terminator region. The coding sequence was codon-optimized for expression in yeast using the proprietary codon optimization program on the Integrated DNA Technologies website. Our design was intended to induce a strong expression of Cripto-1 in yeast to test for membrane localization. We hope that our contribution of a eukaryotic receptor protein to the iGEM Registry is found to be useful and beneficial.

Parts



Part Name Part Description
BBa_K592009 Amil CP, Blue chromaprotein
BB_J23119 Constitutive Promoter
BBa_B0015 Terminator
BBa_E0040 GFP
BBa_K1908000 BY-Toggle
BBa_K1908001 Cripto-1 Coding Sequence
BBa_K1908002 TBA