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Revision as of 09:48, 19 October 2016
![title_img](https://static.igem.org/mediawiki/2016/c/cf/T--BIT-China--title_img.png)
![circle_img](https://static.igem.org/mediawiki/2016/b/ba/T--BIT-China--circle_logo_under.png)
![circle_img](https://static.igem.org/mediawiki/2016/9/9c/T--BIT-China--circle_logo_center.png)
![circle_img](https://static.igem.org/mediawiki/2016/a/a8/T--BIT-China--circle_logo_up.png)
![home_title_down](https://static.igem.org/mediawiki/2016/e/e5/T--BIT-China--img--home_title_down.png)
Plasmid-Sensing Logically and Adjustably Cell Killer
Monitoring Plasmid Numbers
Kill the Slacker
![overtop](https://static.igem.org/mediawiki/2016/1/10/T--BIT-China--over_top.png)
![overview1](https://static.igem.org/mediawiki/2016/1/14/T--BIT-China--img--home_overview1.png)
production, due to the random distribution of
plasmids in the process of bacteria proliferation
and division, the bacteria containing less
plasmids will gradually become the
“slackers”.
![overview2](https://static.igem.org/mediawiki/2016/6/60/T--BIT-China--img--home_overview2.png)
bacteria become the dominant population.
There is no efficient way to prevent the birth of
plasmid-free bacteria and the applied ways are
only limited to the laboratory-use.
![overview3](https://static.igem.org/mediawiki/2016/b/b2/T--BIT-China--img--home_overview3.png)
What about equipping the bacteria with a
plasmid-sensing logically adjustable cell killer
(P-SLACKiller)?
plasmid-sensing logically adjustable cell killer
(P-SLACKiller)?
Here is killer’s basic rule:
Kill the slackers (Plasmid numbers are above a
threshold);
Save the workers (Plasmid numbers are below
the threshold).
Kill the slackers (Plasmid numbers are above a
threshold);
Save the workers (Plasmid numbers are below
the threshold).
![overview4](https://static.igem.org/mediawiki/2016/8/8d/T--BIT-China--img--home_overview4.png)
Surprise! We can guarantee
the microbial
population structure stay optimal now!
population structure stay optimal now!
![overview5](https://static.igem.org/mediawiki/2016/4/47/T--BIT-China--img--home_overview5.png)
Our team divide the experiment into 4 parts as below.
Why not click and have a look?
![home-basic](https://static.igem.org/mediawiki/2016/7/72/T--BIT-China--img--home_pslackiller.png)
Our P-SLACKiller
Plasmids are losing, and the production efficiency is decreasing. Let’s equip the bacteria with a plasmid-sensing logically adjustable killer (P-SLACKiller) to kill slackers with less plasmids among bacteria!
![home-basic](https://static.igem.org/mediawiki/2016/0/0f/T--BIT-China--img--home_threshold.png)
Threshold Device
In what situation can the bacteria be judged as slackers? There must have a threshold concerning plasmid numbers. Let’s find it out and adjust it accordingly.
![home-basic](https://static.igem.org/mediawiki/2016/2/2c/T--BIT-China--img--home_killer.png)
Killer Device
For the slackers, they should be able to turn on the self-killing switch. We tried toxin proteins and double strand break (DSB) to produce the lethal effect.
![home-basic](https://static.igem.org/mediawiki/2016/a/a1/T--BIT-China--img--home_optimization.png)
Optimization
The executor killer should be stably maintained, so we integrated killer device into the genome. Moreover, we mutated promoters to adjust the threshold.