Difference between revisions of "Team:Stony Brook/Notebook"

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<h1> Will add a table to this later </h1>
 
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<h4> 7/2 </h4>
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<h5> Cancer Group </h5>
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<li> Running yesterday's digestion on gel </li>
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Revision as of 17:46, 13 July 2016

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Document the dates you worked on your project.

What should this page have?
  • Chronological notes of what your team is doing.
  • Brief descriptions of daily important events.
  • Pictures of your progress.
  • Mention who participated in what task.
Inspiration

You can see what others teams have done to organize their notes:

Week 1 (6/27 - 7/1)

6/27

Cancer Group
  • PCR of 3xHA-TDGF1 construct
  • Gel of PCR Product → PCR was a failure
  • Second PCR of 3xHA-TDGF1 construct → left overnight
Interlab Study
  • Measured LUDOX and water for plate reader
  • Transformation of positive and negative controls, test devices 1-3

6/28

Cancer Group
  • Ran gel on PCR of yesterday's construct → Gel worked
  • Gel purification of PCR Product → low concentration of DNA found during nanodrop
  • PCR the construct using a revised method
  • 2:54pm → ran the gel on the PCR product
Interab Study
  • Heat and "SB" streaking seems not to have worked
  • No visible red colonies on plate

6/29

Cancer Group
  • PCR Not working well → keep trying new methods with different annealing temperatures
  • Tried 65 and 67 degrees C
Interlab Study
  • FITC started
  • Cutting re-inoculated
  • Ordered new LUDOX
Other
  • College of Arts and Sciences Pre College Summer Institute students stopped by lab
  • Spoke to them about information on synthetic biology and iGEM

6/30

Cancer Group
  • PCR worked → Test 5 → changed annealing temperature to 66 degrees C + increased the denaturing time
  • Nanodropped DNA

7/1

Cancer Group
  • Digestion of TDGF-1 construct and YFP352GAP vector

    • Will add a table to this later

7/2

Cancer Group
  • Running yesterday's digestion on gel