Difference between revisions of "Team:SUSTech Shenzhen/InterLab"
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{{SUSTech_Image_Center | filename=T--SUSTech_Shenzhen--9247196A-FE86-4BF7-98C5-9BEF806BAEB1.png | caption=Fig. 3 FITC fluorescence peak of positive control | width=1000px}} | {{SUSTech_Image_Center | filename=T--SUSTech_Shenzhen--9247196A-FE86-4BF7-98C5-9BEF806BAEB1.png | caption=Fig. 3 FITC fluorescence peak of positive control | width=1000px}} | ||
{{SUSTech_Image_Center | filename=T--SUSTech_Shenzhen--FAA88036-4284-4532-9C21-2479A04DE1A8.png | caption=Fig. 4 FITC fluorescence peak of Device 2 | width=1000px}} | {{SUSTech_Image_Center | filename=T--SUSTech_Shenzhen--FAA88036-4284-4532-9C21-2479A04DE1A8.png | caption=Fig. 4 FITC fluorescence peak of Device 2 | width=1000px}} | ||
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Revision as of 14:59, 19 October 2016
Interlab
Contribution
Contents
Flow Cytometer Measurement
Materials
- 194.7 g FITC (provided in kit)
- 10ml 1xPBS (phosphate buffered saline) 96 well plate
- Competent cells (Escherichia coli strain DH5α)
- LB (Luria Bertani) media with Chloramphenicol (stock concentration 25 mg/mL dissolved in EtOH), 1 ml Falcon tube for cell growth Incubator at 37°C, 1.5 ml eppendorf tubes for sample storage Ice bucket with ice,Pipettes, SpheroTech Rainbow Calibration Particles RCP-30-5A, CytoFlex flow cytometer
Devices (from InterLab Measurement Kit):
- Positive control
- Negative control
- Device 1: J23101+I13504
- Device 2: J23106+I13504
- Device 3: J23117+I13504
Methods
Open computer, click cytometer setting, load clean solution and system startup program for initialization.
Load QC(Lot: 45065) falcon tube to do pre-tests.
Load Rainbow beads, set FSC-A-SSA, FSC-A-FSC-H, FITC-A-Count.
Mix 100ul overnight culture with PBS, load samples and examine the fluorescence.
Close experiment and perform daily clean with ddH2O. Exit the software.
Result
Tab. 1 Result of flow cytometer measurement
Fig. 1 FITC fluorescence peaks of Rainbow beads
Fig. 2 FITC fluorescence peak of negative control
Fig. 3 FITC fluorescence peak of positive control
Fig. 4 FITC fluorescence peak of Device 2
