Difference between revisions of "Team:Stony Brook/Notebook"
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Revision as of 20:48, 13 July 2016
Document the dates you worked on your project.
What should this page have?
- Chronological notes of what your team is doing.
- Brief descriptions of daily important events.
- Pictures of your progress.
- Mention who participated in what task.
Inspiration
You can see what others teams have done to organize their notes:
Week 1 (6/27 - 7/2)
6/27
Cancer Group
- PCR of 3xHA-TDGF1 construct
- Gel of PCR Product → PCR was a failure
- Second PCR of 3xHA-TDGF1 construct → left overnight
Interlab Study
- Measured LUDOX and water for plate reader
- Transformation of positive and negative controls, test devices 1-3
6/28
Cancer Group
- Ran gel on PCR of yesterday's construct → Gel worked
- Gel purification of PCR Product → low concentration of DNA found during nanodrop
- PCR the construct using a revised method
- 2:54pm → ran the gel on the PCR product
Interab Study
- Heat and "SB" streaking seems not to have worked
- No visible red colonies on plate
6/29
Cancer Group
- PCR Not working well → keep trying new methods with different annealing temperatures
- Tried 65 and 67 degrees C
Interlab Study
- FITC started
- Cutting re-inoculated
- Ordered new LUDOX
Other
- College of Arts and Sciences Pre College Summer Institute students stopped by lab
- Spoke to them about information on synthetic biology and iGEM
6/30
Cancer Group
- PCR worked → Test 5 → changed annealing temperature to 66 degrees C + increased the denaturing time
- Nanodropped DNA
7/1
Cancer Group
- Digestion of TDGF-1 construct and YFP352GAP vector
Will add a table to this later
7/2
Cancer Group
- Running yesterday's digestion on gel
Week 2 (7/5-7/8)
7/5
Cancer Group
- Ran the PCR'ed construct on a gel with Phire Polymerase → It didn't work
Vaccine Group
- Things
7/6
Cancer group>
- Ran the construct with PCR on gel → it worked
- Tasnia stabbed our gel
- Interlab study continues
Vaccine Group
- Things
7/7
Cancer group>
- Nanodrops were pretty low (84-120 ng/nl)
- 11:46am PCR'ed the highest nanodrop result in tube #1 → PCR successful
- 2:33pm → attempt to digest with remaining non-PCR product → removed from incubator at 5:00pm
- Made a gel → ran PCR
Vaccine Group
- Things
Week 3 (7/11-7//15)
7/11
Cancer group>
- Ran PCR on the construct (5x) → gel did not work
- PCR'ed 84.2 ng/ml construct
- 5 replicates made using Phire
Insert Table
- Restreaking of YEP352GAP transformed cells for miniprep
- Restreaking placed in incubator at 12am
Vaccine Group
- Stuff & Things
7/12
Cancer group>
- Made gel for electrophoresis of PCR construct → it didn't work
Insert table here
- LB liquid culture (3ml with 3ul of ampicillin)
- Loaded 10ul Ladder DNA.
- Loaded 1ul dye + 5ul PCR product
- Ran gel at 115V
- Checked gel ladder → faint, no band for the PCR product
- Made new gel, loaded with the same amount of reagents as before → ran at 90V → No ladder band
- Ran it again at 115V → visible ladder, no PCR band
- Preparing a gel to run ladder and construct that previously showed a strong band → used this to diagnose a problem with gel setup. Ran TDGF1 152.9 Phire Child. Lane 1 is ladder, Lane 2 is that construct (10ul) with 2ul of dye
- Setup new PCR using the 6/30/16 PCR construct
- 98°C → 30sec
- 98°C → 20sec
- 66°C → 5sec
- 72°C → 30sec
- 72°C → 1min
- 4°C → hold
Vaccine Group
- Stuff & Things
7/13
Cancer group>
- Ran 5ul of PCR with ladder, cancer construct and vaccine construct → to diagnose problem with gel
- Miniprep of Dean vector (3ml LB culture)
