Difference between revisions of "Team:ShanghaitechChina/Hydrogen"

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   <h1 align="center">Connection to the Project</h1>
 
   <h1 align="center">Connection to the Project</h1>
In our sun-powered biofilm-interfaced hydrogen-producing system, <strong>hydrogenase  harnessed in engineered E. coli are conceived to efficiently catalyze proton reduction upon receiving electrons originally donated by semiconductor nanomaterials</strong>. Electron transportation from semiconductors to hydrogenase could be bridged and facilitated by the use of mediators, methyl viologen. To achieve efficient enzymatic activities, we codon-optimized and constructed the whole hydrogenase gene clusters (from Clostridium Acetobutylicum) by leveraging the multi-expression Acembl System. <p></p>
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In our sun-powered biofilm-interfaced hydrogen-producing system, <strong>hydrogenase  harnessed in engineered <i>E.coli</i> are conceived to efficiently catalyze proton reduction upon receiving electrons originally donated by semiconductor nanomaterials</strong>. Electron transportation from semiconductors to hydrogenase could be bridged and facilitated by the use of mediators, methyl viologen. To achieve efficient enzymatic activities, we codon-optimized and constructed the whole hydrogenase gene clusters (from <i>Clostridium acetobutylicum</i>) by leveraging the multi-expression Acembl System. <p></p>
 
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             At molecular level, the gene sequences involved in producing hydrogenase in different species vary wildly. In our study, we focus on hydrogenase gene cluster from Clostridium acetobutylicum. The important genes include hydA, hydEF, hydG, which are expressed as HydA, HydE and HydF, HydG respectively.  We will briefly introduce these enzymes below. (Tip:click enzymes to have fun:)<p></p>
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             At molecular level, the gene sequences involved in producing hydrogenase in different species vary wildly. In our study, we focus on hydrogenase gene cluster from <i>Clostridium acetobutylicum</i>. The important genes include hydA, hydEF, hydG, which are expressed as HydA, HydE and HydF, HydG respectively.  We will briefly introduce these enzymes below. (Tip:click enzymes to have fun:)<p></p>
  
  
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Our goal is to transplant the gene clusters of [FeFe]-hydrogenase from Clostridium acetobutylicum into <em>E. coli</em>, and engineer a strain that could effectively produce hydrogen. Previous work for transferring [FeFe]-hydrogenase into E. coli using a two-plasmid system been demonstrated by Yuki Honda, et al. [4]  Specifically, they used the pETDuet-1 and pCDFDuet-1 system to carry the hydEA and hydFG sequence separately.  However, their method for gene manipulation was laborious and the results were not efficient, as expression of HydA, HydE, HydF, HydG is not controlled in a synchronized way. In addition, the two-plasmid system runs certain risk in the stability of the strain[4]. We made significant improvements on the system using a high-efficiency and multi-expression Acembl system by leveraging the power of synthetic biology, .<p></p>
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Our goal is to transplant the gene clusters of [FeFe]-hydrogenase from <i>Clostridium acetobutylicum</i> into <em>E. coli</em>, and engineer a strain that could effectively produce hydrogen. Previous work for transferring [FeFe]-hydrogenase into <i>E.coli</i> using a two-plasmid system been demonstrated by Yuki Honda, et al. [4]  Specifically, they used the pETDuet-1 and pCDFDuet-1 system to carry the hydEA and hydFG sequence separately.  However, their method for gene manipulation was laborious and the results were not efficient, as expression of HydA, HydE, HydF, HydG is not controlled in a synchronized way. In addition, the two-plasmid system runs certain risk in the stability of the strain[4]. We made significant improvements on the system using a high-efficiency and multi-expression Acembl system by leveraging the power of synthetic biology, .<p></p>
 
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Revision as of 22:15, 19 October 2016

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