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<li> All chemicals, such as Ethidium Bromide and Glacial Acetic Acid were kept in specified areas and were carried in secondary containers as well to minimize risk of spilling </li> | <li> All chemicals, such as Ethidium Bromide and Glacial Acetic Acid were kept in specified areas and were carried in secondary containers as well to minimize risk of spilling </li> | ||
+ | <li> Biological waste was disposed of appropriately, especially if such waste included <i>E. Coli</i> and/or <i>S. Cerevisiae</i></li> | ||
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Latest revision as of 20:00, 19 October 2016
Safety
BSL-1 Organisms
- Stony Brook iGEM worked with E. coli and S. cerevisiae
- We complied with BSL-1 guidelines and wore the required PPE around the lab when handling those organisms
- Nitrile gloves
- Closed-toe shoes and long pants
- UV-protective glasses/goggles for DNA gel analysis
- Gloves were changed after working with said organisms to prevent cross contamination
- No organisms were brought out of the lab at any time
Handling of chemicals
- All chemicals, such as Ethidium Bromide and Glacial Acetic Acid were kept in specified areas and were carried in secondary containers as well to minimize risk of spilling
- Biological waste was disposed of appropriately, especially if such waste included E. Coli and/or S. Cerevisiae
Non-Pathogenic Parts
- Constructs ordered from IDT or otherwise synthesized were made from coding sequences that code for non-pathogenic or infectious molecules