Difference between revisions of "Team:NTU-Singapore/Basic Part"
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Revision as of 00:44, 20 October 2016
Basic Parts
Truncated dCas9
BBa_K2130000 ∆RuvCIII-2 ∆HNH SP-dCas9
This tuncated version of dCas9 is the smallest dCas9 that works the best. The ∆RuvCIII-2 truncation has been rationally predicted by comparison of crystal structures between two states of Cas9.
Conformations of RuvCIII in state A, before NTS cleavage and state B, after NTS cleavage
It is able to retain ~70% of binding strength with 15% of the protein is deleted.
Activation of a zsGreen reporter gene using the various RuvCIII truncations
Microscope image of HEK293FT cells 24 hours after transfection.
