Line 66: | Line 66: | ||
<td> Initial Denaturation </td> | <td> Initial Denaturation </td> | ||
<td> 98 </td> | <td> 98 </td> | ||
− | <td> | + | <td> # </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> Denaturation </td> | <td> Denaturation </td> | ||
<td> 98 </td> | <td> 98 </td> | ||
− | <td> | + | <td> # </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> Annealing </td> | <td> Annealing </td> | ||
− | <td> | + | <td> # </td> |
− | <td> | + | <td> # </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> Extension </td> | <td> Extension </td> | ||
<td> 72 </td> | <td> 72 </td> | ||
− | <td> | + | <td> # </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> Final Extension </td> | <td> Final Extension </td> | ||
<td> 72 </td> | <td> 72 </td> | ||
− | <td> | + | <td> # </td> |
</tr> | </tr> | ||
</table> | </table> | ||
Line 101: | Line 101: | ||
<tr> | <tr> | ||
<td> Construct </td> | <td> Construct </td> | ||
− | <td> | + | <td> # </td> |
− | <td> | + | <td> # </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> Vector </td> | <td> Vector </td> | ||
− | <td> | + | <td> # </td> |
− | <td> | + | <td> # </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
Line 122: | Line 122: | ||
<tr> | <tr> | ||
<td> 10X T4 Buffer </td> | <td> 10X T4 Buffer </td> | ||
− | <td> | + | <td> # </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> Vector </td> | <td> Vector </td> | ||
− | <td> | + | <td> # (# ng) </td> |
</tr> | </tr> | ||
<td> Construct </td> | <td> Construct </td> | ||
− | <td> | + | <td> # (# ng) </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> H<sub>2</sub>O</td> | <td> H<sub>2</sub>O</td> | ||
− | <td> | + | <td> # </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> Ligase </td> | <td> Ligase </td> | ||
− | <td> | + | <td> #</td> |
</tr> | </tr> | ||
</table> | </table> | ||
Line 152: | Line 152: | ||
<div class="column half_size"> | <div class="column half_size"> | ||
<div align="center"> | <div align="center"> | ||
− | <h5> | + | <h5> This </h5> |
</div> | </div> | ||
<ul> | <ul> | ||
Line 163: | Line 163: | ||
<tr> | <tr> | ||
<td> H<sub>2</sub>O </td> | <td> H<sub>2</sub>O </td> | ||
− | <td> | + | <td> #</td> |
− | <td> | + | <td> # </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> Cutsmart </td> | <td> Cutsmart </td> | ||
− | <td> | + | <td> # </td> |
− | <td> | + | <td> # </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> EcoRI-HF </td> | <td> EcoRI-HF </td> | ||
− | <td> | + | <td> # </td> |
<td> X </td> | <td> X </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> XbaI </td> | <td> XbaI </td> | ||
− | <td> | + | <td># </td> |
<td> X </td> | <td> X </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> DNA </td> | <td> DNA </td> | ||
− | <td> | + | <td> # </td> |
− | <td> | + | <td> # </td> |
</tr> | </tr> | ||
</table> | </table> | ||
Line 192: | Line 192: | ||
<div class="column half_size"> | <div class="column half_size"> | ||
<div align="center"> | <div align="center"> | ||
− | <h5> | + | <h5> A </h5> |
</div> | </div> | ||
<ul> | <ul> | ||
Line 203: | Line 203: | ||
<tr> | <tr> | ||
<td> H<sub>2</sub>O </td> | <td> H<sub>2</sub>O </td> | ||
− | <td> | + | <td> #</td> |
− | <td> | + | <td> # </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> Cutsmart </td> | <td> Cutsmart </td> | ||
− | <td> | + | <td> # </td> |
− | <td> | + | <td> # </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> EcoRI-HF </td> | <td> EcoRI-HF </td> | ||
− | <td> | + | <td> # </td> |
<td> X </td> | <td> X </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> XbaI </td> | <td> XbaI </td> | ||
− | <td> | + | <td># </td> |
<td> X </td> | <td> X </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> DNA </td> | <td> DNA </td> | ||
− | <td> | + | <td> # </td> |
− | <td> | + | <td> # </td> |
</tr> | </tr> | ||
</table> | </table> | ||
Line 236: | Line 236: | ||
<div class="column half_size"> | <div class="column half_size"> | ||
<div align="center"> | <div align="center"> | ||
− | <h5> | + | <h5> Is </h5> |
</div> | </div> | ||
<ul> | <ul> | ||
Line 247: | Line 247: | ||
<tr> | <tr> | ||
<td> H<sub>2</sub>O </td> | <td> H<sub>2</sub>O </td> | ||
− | <td> | + | <td> #</td> |
− | <td> | + | <td> # </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> Cutsmart </td> | <td> Cutsmart </td> | ||
− | <td> | + | <td> # </td> |
− | <td> | + | <td> # </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> EcoRI-HF </td> | <td> EcoRI-HF </td> | ||
− | <td> | + | <td> # </td> |
<td> X </td> | <td> X </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> XbaI </td> | <td> XbaI </td> | ||
− | <td> | + | <td># </td> |
<td> X </td> | <td> X </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> DNA </td> | <td> DNA </td> | ||
− | <td> | + | <td> # </td> |
− | <td> | + | <td> # </td> |
</tr> | </tr> | ||
</table> | </table> | ||
Line 276: | Line 276: | ||
<div class="column half_size"> | <div class="column half_size"> | ||
<div align="center"> | <div align="center"> | ||
− | <h5> | + | <h5> Thing </h5> |
</div> | </div> | ||
<ul> | <ul> | ||
Line 287: | Line 287: | ||
<tr> | <tr> | ||
<td> H<sub>2</sub>O </td> | <td> H<sub>2</sub>O </td> | ||
− | <td> | + | <td> #</td> |
− | <td> | + | <td> # </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> Cutsmart </td> | <td> Cutsmart </td> | ||
− | <td> | + | <td> # </td> |
− | <td> | + | <td> # </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> EcoRI-HF </td> | <td> EcoRI-HF </td> | ||
− | <td> | + | <td> # </td> |
<td> X </td> | <td> X </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> XbaI </td> | <td> XbaI </td> | ||
− | <td> | + | <td># </td> |
<td> X </td> | <td> X </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> DNA </td> | <td> DNA </td> | ||
− | <td> | + | <td> # </td> |
− | <td> | + | <td> # </td> |
</tr> | </tr> | ||
</table> | </table> |
Revision as of 07:50, 5 August 2016
I left you some of the commonly used templates down there under 8/8. Just fill them in as needed. I'll only really have wifi for a few hours on two days between Saturday and Thursday. If you need anything, message me on Facebook and I'll get back to you as soon as I can.
With the warmest love and regards,
~JOSHUA
Week 7: 8/8 - 8/14
8/8
PCR Table Template:
Contents | ul | Phire | # |
---|---|
H2O | # |
50 uM F Primer | # |
50 uM R Primer | # |
#ug/ul Construct | # |
PCR Settings Template:
Phase | Temperature (°C) | Time (sec) |
---|---|---|
Initial Denaturation | 98 | # |
Denaturation | 98 | # |
Annealing | # | # |
Extension | 72 | # |
Final Extension | 72 | # |
Nanodrop Template:
Content | Concentration (ng/ul) | 260/280 |
---|---|---|
Construct | # | # |
Vector | # | # |
Ligation Template
Content | ul |
---|---|
10X T4 Buffer | # |
Vector | # (# ng) | Construct | # (# ng) |
H2O | # |
Ligase | # |
Multiple Digests Template:
This
Content | Experimental ul | Control ul |
---|---|---|
H2O | # | # |
Cutsmart | # | # |
EcoRI-HF | # | X |
XbaI | # | X |
DNA | # | # |
A
Content | Experimental ul | Control ul |
---|---|---|
H2O | # | # |
Cutsmart | # | # |
EcoRI-HF | # | X |
XbaI | # | X |
DNA | # | # |
Is
Content | Experimental ul | Control ul |
---|---|---|
H2O | # | # |
Cutsmart | # | # |
EcoRI-HF | # | X |
XbaI | # | X |
DNA | # | # |
Thing
Content | Experimental ul | Control ul |
---|---|---|
H2O | # | # |
Cutsmart | # | # |
EcoRI-HF | # | X |
XbaI | # | X |
DNA | # | # |