Line 35: | Line 35: | ||
<div class="column half_size"> | <div class="column half_size"> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <table> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <td><strong>Component</strong></td> | ||
+ | <td><strong>50 µl Reaction</strong></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Nuclease-free water</td> | ||
+ | |||
+ | <td>to 50 µl</td> | ||
+ | |||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>5X Phusion HF</td> | ||
+ | |||
+ | <td>10 µl</td> | ||
+ | |||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>10 mM dNTPs</td> | ||
+ | |||
+ | <td>1 µl</td> | ||
+ | |||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>10 µM Forward Primer</td> | ||
+ | |||
+ | <td>2.5 µl</td> | ||
+ | |||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>10 µM Reverse Primer</td> | ||
+ | |||
+ | <td>2.5 µl</td> | ||
+ | |||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Template DNA</td> | ||
+ | |||
+ | <td>variable</td> | ||
+ | <td>< 250 ng</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>DMSO (optional)</td> | ||
+ | |||
+ | <td>(1.5 µl, up to 3%)</td> | ||
+ | |||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Phusion DNA <br /> | ||
+ | Polymerase</td> | ||
+ | |||
+ | <td>0.5 µl</td> | ||
+ | |||
+ | </tr> | ||
+ | </tbody> | ||
+ | </table> | ||
+ | |||
+ | |||
+ | |||
Revision as of 00:15, 9 October 2016
Wiki under construction
Describe the experiments, research and protocols you used in your iGEM project.
What should this page contain?
- Protocols
- Experiments
- Documentation of the development of your project
Inspiration
Component | 50 µl Reaction | |
Nuclease-free water | to 50 µl | |
5X Phusion HF | 10 µl | |
10 mM dNTPs | 1 µl | |
10 µM Forward Primer | 2.5 µl | |
10 µM Reverse Primer | 2.5 µl | |
Template DNA | variable | < 250 ng |
DMSO (optional) | (1.5 µl, up to 3%) | |
Phusion DNA Polymerase |
0.5 µl |